Fast Raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detection

Corden, Christopher J.; Shipp, Dustin W.; Matousek, Pavel; Notingher, Ioan

Abstract

We present a time-gated Raman micro-spectroscopy technique suitable for fast Raman mapping of samples eliciting large laser-induced fluorescence backgrounds. To achieve the required time resolution for effective fluorescence rejection, a picosecond pulsed laser and a single-photon avalanche diode were used. A module consisting of a spectrometer, digital micromirror device, and two prisms was used for high-resolution spectral filtering and multiplexing, which is required for a high chemical specificity and short integration times. With this instrument, we demonstrated time-gated Raman imaging of highly fluorescent samples, achieving acquisition times as short as 3 min for 40×40
pixel resolution images

Citation

Corden, C. J., Shipp, D. W., Matousek, P., & Notingher, I. (2018). Fast Raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detection. Optics Letters, 43(23), 5733-5736. https://doi.org/10.1364/ol.43.005733

• Journal Article Type: Article
• Acceptance Date: Oct 7, 2018
• Online Publication Date: Nov 21, 2018
• Publication Date: Dec 1, 2018
• Deposit Date: Nov 23, 2018
• Publicly Available Date: Nov 23, 2018
• Journal: Optics Letters
• Print ISSN: 0146-9592
• Electronic ISSN: 1539-4794
• Publisher: Optical Society of America
• Peer Reviewed: Peer Reviewed
• Volume: 43
• Issue: 23
• Pages: 5733-5736
• DOI: https://doi.org/10.1364/ol.43.005733
• Keywords: Atomic and Molecular Physics, and Optics
• Public URL: https://nottingham-repository.worktribe.com/output/1303846
• Publisher URL: https://www.osapublishing.org/ol/fulltext.cfm?uri=ol-43-23-5733&id=401402
• Additional Information: This article is maintained by: OSA - The Optical Society; Crossref DOI link to publisher maintained version: https://doi.org/10.1364/OL.43.005733; Article type: letter; Similarity check: Screened by Similarity Check; Peer reviewed: Yes; Review process: Single blind; Received: 11 September 2018; Accepted: 7 October 2018; Published: 21 November 2018; Copyright: Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.