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Quantification of Drugs in Brain and Liver Mimetic Tissue Models Using Raman Spectroscopy

Woodhouse, Nathan; Majer, Jan; Marshall, Peter; Hood, Steve; Notingher, Ioan

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Authors

Nathan Woodhouse

Jan Majer

Peter Marshall

Steve Hood



Abstract

Quantitative analysis of drug delivery with in biological systems is an integral challenge in drug development. Analytical techniques are important for assessing both drug target delivery, target action, and drug toxicology. Using mimetic tissue models, we have investigated the efficacy of Raman spectroscopy in quantitative detection of alkyne group and deuterated drugs in rat brain and rat liver tissue models. Lasers with 671 nm and 785 nm wavelengths were assessed for their feasibility in this application due to opposing relative benefits and disadvantages. Thin tissue sections have been tested as a practical means of reducing autofluorescent background by minimizing out-of-focus tissue and therefore maximizing photobleaching rates. Alkyne-tagged drugs were quantitatively measured at 18 ± 5 μg/g drug/tissue mass ratio in rat brain and at 34 ± 6 μg/g in rat liver. Quantification calibration curves were generated for a range of concentrations from 0–500 μg/g. These results show the potential of Raman spectroscopy as a diffraction-limited spatially resolved imaging technique for assessing drug delivery in tissue applications.

Citation

Woodhouse, N., Majer, J., Marshall, P., Hood, S., & Notingher, I. (2022). Quantification of Drugs in Brain and Liver Mimetic Tissue Models Using Raman Spectroscopy. Applied Spectroscopy, https://doi.org/10.1177/00037028221139494

Journal Article Type Article
Acceptance Date Sep 29, 2022
Online Publication Date Nov 1, 2022
Publication Date Nov 1, 2022
Deposit Date Nov 11, 2022
Publicly Available Date Nov 17, 2022
Journal Applied Spectroscopy
Print ISSN 0003-7028
Electronic ISSN 1943-3530
Publisher SAGE Publications
Peer Reviewed Peer Reviewed
DOI https://doi.org/10.1177/00037028221139494
Keywords Raman spectroscopy, drug delivery, microscopy, drug quantification
Public URL https://nottingham-repository.worktribe.com/output/13181884
Publisher URL https://journals.sagepub.com/doi/10.1177/00037028221139494

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