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Odontogenic differentiation of human dental pulp stem cells on hydrogel scaffolds derived from decellularized bone extracellular matrix and collagen type I

Paduano, Francesco; Marrelli, Massimo; White, Lisa J.; Shakesheff, Kevin; Tatullo, Marco

Authors

Francesco Paduano

Massimo Marrelli

Kevin Shakesheff

Marco Tatullo



Abstract

Objectives
The aim of this study was to evaluate the level of odontogenic
differentiation of dental pulp stem cells (DPSCs) on hydrogel scaffolds derived from bone extracellular matrix (bECM) in comparison to those seeded on collagen I (Col-I), one of the main components of dental pulp ECM.

Methods
DPSCs isolated from human third molars were characterized for surface marker expression and odontogenic potential prior to seeding into bECM or Col-I hydrogel scaffolds. The cells were then seeded onto bECM and Col-I hydrogel scaffolds and cultured under basal conditions or with odontogenic and growth factor (GF) supplements. DPSCs cultivated on tissue culture polystyrene (TCPS) with and without supplements were used as controls. Gene expression of dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP-1) and matrix extracellular phosphoglycoprotein (MEPE) was evaluated by quantitative reverse transcription-polymerase chain reaction (qRT PCR) and mineral deposition was observed by Von Kossa staining.

Results
When DPSCs were cultured on bECM hydrogels, the mRNA expression levels of DSPP, DMP-1 and MEPE genes were significantly upregulated with respect to those cultured on Col-I scaffolds or TCPS in the absence of extra odontogenic inducers. In addition, more mineral deposition was observed on bECM hydrogel scaffolds as demonstrated by Von Kossa staining. Moreover, DSPP, DMP-1 and MEPE mRNA expressions of DPSCs cultured on bECM hydrogels were further upregulated by the addition of GFs or osteo/odontogenic
medium compared to Col-I treated cells in the same culture conditions.

Significance
These results demonstrate the potential of the bECM hydrogel scaffolds to stimulate odontogenic differentiation of DPSCs.

Citation

Paduano, F., Marrelli, M., White, L. J., Shakesheff, K., & Tatullo, M. (2016). Odontogenic differentiation of human dental pulp stem cells on hydrogel scaffolds derived from decellularized bone extracellular matrix and collagen type I. PLoS ONE, 11(2), 1-18. https://doi.org/10.1371/journal.pone.0148225

Journal Article Type Article
Acceptance Date Jan 14, 2016
Online Publication Date Feb 16, 2016
Publication Date Feb 16, 2016
Deposit Date Sep 14, 2018
Publicly Available Date Mar 28, 2024
Journal PLoS ONE
Electronic ISSN 1932-6203
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 11
Issue 2
Article Number e0148225
Pages 1-18
DOI https://doi.org/10.1371/journal.pone.0148225
Public URL https://nottingham-repository.worktribe.com/output/1109277
Publisher URL https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0148225
PMID 00037121

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