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Ovarian extracellular matrix‐based hydrogel for human ovarian follicle survival in vivo: A pilot work

Chiti, Maria‐Costanza; Vanacker, Julie; Ouni, Emna; Tatic, Natalija; Viswanath, Aiswarya; des Rieux, Anne; Dolmans, Marie‐Madeleine; White, Lisa Jane; Amorim, Christiani Andrade

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Authors

Maria‐Costanza Chiti

Julie Vanacker

Emna Ouni

Natalija Tatic

Aiswarya Viswanath

Anne des Rieux

Marie‐Madeleine Dolmans

Christiani Andrade Amorim



Abstract

To successfully assemble a bio-engineered ovary, we need to create a three-dimensional matrix able to accommodate isolated follicles and cells. The goal of this study was to develop an extracellular matrix hydrogel (oECM) derived from decellularized bovine ovaries able to support, in combination with alginate, human ovarian follicle survival and growth in vitro. Two different hydrogels (oECM1, oECM2) were produced and compared in terms of decellularization efficiency (dsDNA), ECM preservation (collagen and glycosaminoglycan levels), ultrastructure, rigidity, and cytotoxicity. oECM2 showed significantly less dsDNA, greater retention of glycosaminoglycans and better rigidity than oECM1. Isolated human ovarian follicles were then encapsulated in four selected hydrogel combinations: (1) 100% oECM2, (2) 90% oECM2 + 10% alginate, (3) 75% oECM2 + 25% alginate, and (4) 100% alginate. After 1 week of in vitro culture, follicle recovery rate, viability, and growth were analyzed. On day 7 of in vitro culture, follicle recovery rates were 0%, 23%, 65%, 82% in groups 1–4, respectively, rising proportionally with increased alginate content. However, there was no difference in follicle viability or growth between groups 2 and 3 and controls (group 4). In conclusion, since pure alginate cannot be used to graft preantral follicles due to its poor revascularization and degradation after grafting, oECM2 hydrogel combined with alginate may provide a new and promising alternative to graft isolated human follicles in a bio-engineered ovary.

Citation

Chiti, M., Vanacker, J., Ouni, E., Tatic, N., Viswanath, A., des Rieux, A., …Amorim, C. A. (2022). Ovarian extracellular matrix‐based hydrogel for human ovarian follicle survival in vivo: A pilot work. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 110(5), 1012-1022. https://doi.org/10.1002/jbm.b.34974

Journal Article Type Article
Acceptance Date Nov 13, 2021
Online Publication Date Nov 26, 2021
Publication Date May 1, 2022
Deposit Date Dec 6, 2021
Publicly Available Date Mar 29, 2024
Journal Journal of Biomedical Materials Research Part B: Applied Biomaterials
Print ISSN 1552-4973
Electronic ISSN 1552-4981
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 110
Issue 5
Pages 1012-1022
DOI https://doi.org/10.1002/jbm.b.34974
Keywords Biomedical Engineering; Biomaterials
Public URL https://nottingham-repository.worktribe.com/output/6909141
Publisher URL https://onlinelibrary.wiley.com/doi/10.1002/jbm.b.34974
Additional Information Received: 2021-05-04; Accepted: 2021-11-13; Published: 2021-11-26

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