Controlled release of BMP-2 from a sintered polymer scaffold enhances bone repair in a mouse calvarial defect model

Rahman, Cheryl V.; Ben?David, Dror; Dhillon, Amritpaul; Kuhn, Gisela; Gould, Toby; M�ller, Ralph; Rose, Felicity R. A. J.; Shakesheff, Kevin M.; Livne, Erella

Abstract

Sustained and controlled delivery of growth factors, such as bone morphogenetic protein 2 (BMP-2), from polymer scaffolds has excellent potential for enhancing bone regeneration. The present study investigated the use of novel sintered polymer scaffolds prepared using temperature-sensitive PLGA/PEG particles. Growth factors can be incorporated into these scaffolds by mixing the reconstituted growth factor with the particles prior to sintering. The ability of the PLGA/PEG scaffolds to deliver BMP-2 in a controlled and sustained manner was assessed and the osteogenic potential of these scaffolds was determined in a mouse calvarial defect model. BMP-2 was released from the scaffolds in vitro over 3 weeks. On average, ca. 70% of the BMP-2 loaded into the scaffolds was released by the end of this time period. The released BMP-2 was shown to be active and to induce osteogenesis when used in a cell culture assay. A substantial increase in new bone volume of 55% was observed in a mouse calvarial defect model for BMP-2-loaded PLGA/PEG scaffolds compared to empty defect controls. An increase in new bone volume of 31% was observed for PLGA/PEG scaffolds without BMP-2, compared to empty defect controls. These results demonstrate the potential of novel PLGA/PEG scaffolds for sustained BMP-2 delivery for bone-regeneration applications. © 2012 John Wiley & Sons, Ltd.

Citation

Rahman, C. V., Ben‐David, D., Dhillon, A., Kuhn, G., Gould, T., Müller, R., …Livne, E. (2014). Controlled release of BMP-2 from a sintered polymer scaffold enhances bone repair in a mouse calvarial defect model. Journal of Tissue Engineering and Regenerative Medicine, 8(1), 59-66. https://doi.org/10.1002/term.1497

• Journal Article Type: Article
• Acceptance Date: Jan 24, 2012
• Online Publication Date: Jun 8, 2012
• Publication Date: Jan 3, 2014
• Deposit Date: Sep 14, 2018
• Journal: Journal of Tissue Engineering and Regenerative Medicine
• Print ISSN: 1932-6254
• Electronic ISSN: 1932-7005
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 8
• Issue: 1
• Pages: 59-66
• DOI: https://doi.org/10.1002/term.1497
• Keywords: Medicine (miscellaneous); Biomaterials; Biomedical Engineering
• Public URL: https://nottingham-repository.worktribe.com/output/1095324
• Publisher URL: https://onlinelibrary.wiley.com/doi/full/10.1002/term.1497
• PMID: 00032919