Outputs (28)

Cross-chemistry leads to product diversity from atromentin synthetases in Aspergilli from section Nigri (2018)
Journal Article
Geib, E., Baldeweg, F., Doerfer, M., Nett, M., & Brock, M. (2019). Cross-chemistry leads to product diversity from atromentin synthetases in Aspergilli from section Nigri. Cell Chemical Biology, 26(2), 223-234.e6. https://doi.org/10.1016/j.chembiol.2018.10.021

Nonribosomal peptide synthetase (NRPS)-like enzymes catalyse the non-oxidative homodimerisation of aromatic α-keto acids, but the exact reaction mechanism is unknown. The furanone-forming thioesterase domain of the Aspergillus terreus aspulvinone E s... Read More about Cross-chemistry leads to product diversity from atromentin synthetases in Aspergilli from section Nigri.

Monitoring of fluconazole and caspofungin activity against in vivo Candida glabrata biofilms by bioluminescence imaging (2018)
Journal Article
Persyn, A., Rogiers, O., Brock, M., Vande Velde, G., Lamkanfi, M., Jacobsen, I. D., Himmelreich, U., Lagrou, K., Van Dijck, P., & Kucharíková, S. (2018). Monitoring of fluconazole and caspofungin activity against in vivo Candida glabrata biofilms by bioluminescence imaging. Antimicrobial Agents and Chemotherapy, 63(2), https://doi.org/10.1128/aac.01555-18

Candida glabrata can attach to various medical implants and forms thick biofilms despite its inability to switch from-yeast-to hyphae. Current in vivo C. glabrata biofilm models only provide limited information about colonization and infection and us... Read More about Monitoring of fluconazole and caspofungin activity against in vivo Candida glabrata biofilms by bioluminescence imaging.

Hybrid in silico/in vitro target fishing to assign function to “orphan” compounds of food origin: the case of the fungal metabolite atromentin (2018)
Journal Article
Dellafiora, L., Aichinger, G., Geib, E., Sánchez-Barrionuevo, L., Brock, M., Cánovas, D., Dall'Asta, C., & Marko, D. (2019). Hybrid in silico/in vitro target fishing to assign function to “orphan” compounds of food origin: the case of the fungal metabolite atromentin. Food Chemistry, 270, 61-69. https://doi.org/10.1016/j.foodchem.2018.07.027

Many small molecules of food origin may effect human health but lack an adequate description of their biological activity. To fill this knowledge gap, a first-line workflow is needed to assign putative functions, rank the endpoints for testing and gu... Read More about Hybrid in silico/in vitro target fishing to assign function to “orphan” compounds of food origin: the case of the fungal metabolite atromentin.

A Multimodal Imaging Approach Enables In Vivo Assessment of Antifungal Treatment in a Mouse Model of Invasive Pulmonary Aspergillosis (2018)
Journal Article
Poelmans, J., Himmelreich, U., Vanherp, L., Zhai, L., Hillen, A., Holvoet, B., Belderbos, S., Brock, M., Maertens, J., Velde, G. V., & Lagrou, K. (2018). A Multimodal Imaging Approach Enables In Vivo Assessment of Antifungal Treatment in a Mouse Model of Invasive Pulmonary Aspergillosis. Antimicrobial Agents and Chemotherapy, 62(7), Article AAC.00240-18. https://doi.org/10.1128/AAC.00240-18

Aspergillus fumigatus causes life-threatening lung infections in immunocompromised patients. Mouse models are extensively used in research to assess the in vivo efficacy of antifungals. In recent years, there has been an increasing interest in the us... Read More about A Multimodal Imaging Approach Enables In Vivo Assessment of Antifungal Treatment in a Mouse Model of Invasive Pulmonary Aspergillosis.

ATNT: an enhanced system for expression of polycistronic secondary metabolite gene clusters in Aspergillus niger (2017)
Journal Article
Geib, E., & Brock, M. (in press). ATNT: an enhanced system for expression of polycistronic secondary metabolite gene clusters in Aspergillus niger. Fungal Biology and Biotechnology, 4(1), Article e13. https://doi.org/10.1186/s40694-017-0042-1

Background: Fungi are treasure chests for yet unexplored natural products. However, exploitation of their real potential remains difficult as a significant proportion of biosynthetic gene clusters appears silent under standard laboratory conditions.... Read More about ATNT: an enhanced system for expression of polycistronic secondary metabolite gene clusters in Aspergillus niger.

Candida albicans Hap43 domains are required under iron starvation but not excess (2017)
Journal Article
Skrahina, V., Brock, M., Hube, B., & Brunke, S. (2017). Candida albicans Hap43 domains are required under iron starvation but not excess. Frontiers in Microbiology, 8, Article 2388. https://doi.org/10.3389/fmicb.2017.02388

Iron availability is a central factor in infections, since iron is a critical micronutrient for all living organisms. The host employs both iron limitation and toxicity strategies to control microbial growth, and successful pathogens are able to tigh... Read More about Candida albicans Hap43 domains are required under iron starvation but not excess.

Persistence within dendritic cells marks an antifungal evasion and dissemination strategy of Aspergillus terreus (2017)
Journal Article
Hsieh, S.-H., Kurzai, O., & Brock, M. (2017). Persistence within dendritic cells marks an antifungal evasion and dissemination strategy of Aspergillus terreus. Scientific Reports, 7, https://doi.org/10.1038/s41598-017-10914-w

Aspergillus terreus is an airborne human fungal pathogen causing life-threatening invasive aspergillosis in immunocompromised patients. In contrast to Aspergillus fumigatus, A. terreus infections are associated with high dissemination rates and poor... Read More about Persistence within dendritic cells marks an antifungal evasion and dissemination strategy of Aspergillus terreus.

A highly conserved basidiomycete peptide synthetase produces a trimeric hydroxamate siderophore (2017)
Journal Article
Brandenburger, E., Gressler, M., Leonhardt, R., Lackner, G., Habel, A., Hertweck, C., Brock, M., & Hoffmeister, D. (in press). A highly conserved basidiomycete peptide synthetase produces a trimeric hydroxamate siderophore. Applied and Environmental Microbiology, https://doi.org/10.1128/AEM.01478-17

The model white-rot basidiomycete Ceriporiopsis (Gelatoporia) subvermispora B encodes putative natural product biosynthesis genes. Among them is the gene for the seven-domain nonribosomal peptide synthetase CsNPS2. It is a member of the as-yet unchar... Read More about A highly conserved basidiomycete peptide synthetase produces a trimeric hydroxamate siderophore.

Lipid components of bile increase the protective effect of conjugated bile salts against antifungal drugs (2017)
Journal Article
Hsieh, S.-H., & Brock, M. (in press). Lipid components of bile increase the protective effect of conjugated bile salts against antifungal drugs. Fungal Biology, https://doi.org/10.1016/j.funbio.2017.08.002

Fungi and bacteria can persist in the human gall bladder. Previous studies have shown that bile protects Candida albicans in this cryptic host niche from antifungals, providing a reservoir for intestinal re-colonization after discontinuation of antif... Read More about Lipid components of bile increase the protective effect of conjugated bile salts against antifungal drugs.

Encapsulation of antifungals in micelles protects Candida albicans during gall-bladder infection (2017)
Journal Article
Hsieh, S.-H., Brunke, S., & Brock, M. (2017). Encapsulation of antifungals in micelles protects Candida albicans during gall-bladder infection. Frontiers in Microbiology, 8, Article 117. https://doi.org/10.3389/fmicb.2017.00117

Candida albicans is a dimorphic fungus that colonizes human mucosal surfaces with the potential to cause life-threatening invasive candidiasis. Studies on systemic candidiasis in a murine infection model using in vivo real-time bioluminescence imagin... Read More about Encapsulation of antifungals in micelles protects Candida albicans during gall-bladder infection.