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Monitoring of fluconazole and caspofungin activity against in vivo Candida glabrata biofilms by bioluminescence imaging

Persyn, Aranka; Rogiers, Ona; Brock, Matthias; Vande Velde, Greetje; Lamkanfi, Mohamed; Jacobsen, Ilse D.; Himmelreich, Uwe; Lagrou, Katrien; Van Dijck, Patrick; Kuchar�kov�, So?a

Authors

Aranka Persyn

Ona Rogiers

Greetje Vande Velde

Mohamed Lamkanfi

Ilse D. Jacobsen

Uwe Himmelreich

Katrien Lagrou

Patrick Van Dijck

So?a Kuchar�kov�



Abstract

Candida glabrata can attach to various medical implants and forms thick biofilms despite its inability to switch from-yeast-to hyphae. Current in vivo C. glabrata biofilm models only provide limited information about colonization and infection and usually require animal sacrifice. To gain real-time information from individual BALB/c mice we developed a non-invasive imaging technique to visualize C. glabrata biofilms in catheter fragments that were subcutaneously implanted on the back of mice. Bioluminescent C. glabrata reporter strains (lucOPT 7/2/4 and lucOPT 8/1/4), free of auxotrophic markers, expressing a codon-optimized firefly luciferase were generated. A murine subcutaneous model was used to follow real-time in vivo biofilm formation in the presence and absence of fluconazole and caspofungin. Fungal load in biofilms was quantified by colony forming unit counts and by bioluminescence imaging (BLI). C. glabrata biofilms formed within the first 24 h, as documented by the increased number of device-associated cells and elevated bioluminescent signal compared to adhesion at the time of implant. The in vivo model allowed monitoring of the anti-biofilm activity of caspofungin against C. glabrata biofilms through bioluminescent imaging from day four after initiation of treatment. Contrarily, signals emitted from biofilms implanted in fluconazole-treated mice was similar to the light emitted from control-treated mice.
This study gives insights into real-time development of C. glabrata biofilms under in vivo conditions. BLI proved to be a dynamic, non-invasive and sensitive tool to monitor continuous biofilm formation and activity of antifungal agents against C. glabrata biofilms formed on abiotic surfaces in vivo.

Citation

Persyn, A., Rogiers, O., Brock, M., Vande Velde, G., Lamkanfi, M., Jacobsen, I. D., …Kucharíková, S. (2018). Monitoring of fluconazole and caspofungin activity against in vivo Candida glabrata biofilms by bioluminescence imaging. Antimicrobial Agents and Chemotherapy, 63(2), https://doi.org/10.1128/aac.01555-18

Journal Article Type Article
Acceptance Date Oct 27, 2018
Online Publication Date Nov 12, 2018
Publication Date Nov 12, 2018
Deposit Date Nov 20, 2018
Publicly Available Date Nov 20, 2018
Journal Antimicrobial Agents and Chemotherapy
Print ISSN 0066-4804
Electronic ISSN 1098-6596
Publisher American Society for Microbiology
Peer Reviewed Peer Reviewed
Volume 63
Issue 2
DOI https://doi.org/10.1128/aac.01555-18
Keywords Pharmacology (medical); Pharmacology; Infectious diseases
Public URL https://nottingham-repository.worktribe.com/output/1288274
Publisher URL https://aac.asm.org/content/early/2018/11/08/AAC.01555-18

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