Outputs (167)

Optimization of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in a small animal model (2023)
Journal Article
Dooley, M., Luckett, J., Alexander, M. R., Matousek, P., Dehghani, H., Ghaemmaghami, A. M., & Notingher, I. (2023). Optimization of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in a small animal model. Biomedical Optics Express, 14(12), 6592-6606. https://doi.org/10.1364/boe.512118

Diffuse Raman spectroscopy (DRS) allows subsurface molecular analysis of optically turbid samples. Numerical modeling of light propagation was used as a method for improving the design of an DRS instrument to maximize the signal to noise ratio (SNR)... Read More about Optimization of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in a small animal model.

Optimisation of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in small animal model (2023)
Journal Article
Dooley, M., Luckett, J., Alexander, M. R., Matousek, P., Dehghani, H., Ghaemmaghami, A. M., & Notingher, I. (2023). Optimisation of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in small animal model. Biomedical Optics Express, 14(12), 6592-6606. https://doi.org/10.1364/BOE.512118

Diffuse Raman spectroscopy (DRS) allows subsurface molecular analysis of optically turbid samples. Numerical modelling of light propagation was used as a method for improving the design of an DRS instrument to maximize the signal to noise ratio (SNR)... Read More about Optimisation of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in small animal model.

ThermoBRET: A Ligand-Engagement Nanoscale Thermostability Assay Applied to GPCRs** (2023)
Journal Article
Hoare, B. L., Tippett, D. N., Kaur, A., Cullum, S. A., Miljuš, T., Koers, E. J., …Veprintsev, D. B. (2024). ThermoBRET: A Ligand-Engagement Nanoscale Thermostability Assay Applied to GPCRs**. ChemBioChem, 25(2), Article e202300459. https://doi.org/10.1002/cbic.202300459

Measurements of membrane protein thermostability reflect ligand binding. Current thermostability assays often require protein purification or rely on pre-existing radiolabelled or fluorescent ligands, limiting their application to established targets... Read More about ThermoBRET: A Ligand-Engagement Nanoscale Thermostability Assay Applied to GPCRs**.

Sustained Release of Dexamethasone from 3D-Printed Scaffolds Modulates Macrophage Activation and Enhances Osteogenic Differentiation (2023)
Journal Article
Majrashi, M., Yang, J., Ghaemmaghami, A., Kotowska, A., Hicks, J. M., & Scurr, D. (2023). Sustained Release of Dexamethasone from 3D-Printed Scaffolds Modulates Macrophage Activation and Enhances Osteogenic Differentiation. ACS Applied Materials and Interfaces, 15(49), 56623–56638. https://doi.org/10.1021/acsami.3c09774

Enhancing osteogenesis via modulating immune cells is emerging as a new approach to address current challenges in repairing bone defects and fractures. However, much remains unknown about the crosstalk between immune cells and osteolineage cells duri... Read More about Sustained Release of Dexamethasone from 3D-Printed Scaffolds Modulates Macrophage Activation and Enhances Osteogenic Differentiation.

Co-assembling living material as an in vitro lung epithelial infection model (2023)
Journal Article
Wu, Y., Romero, M., Robertson, S. N., Fenn, S., Fisher, L., Willingham, I., …Mata, A. (2024). Co-assembling living material as an in vitro lung epithelial infection model. Matter, 7(1), 216-236. https://doi.org/10.1016/j.matt.2023.10.029

Biofilms are robust living 3D materials that play key roles in nature but also cause major problems, such as tolerance to antibiotic treatment. Recreation of these living structures in vitro is critical to understand their biology and develop solutio... Read More about Co-assembling living material as an in vitro lung epithelial infection model.

Differential interaction patterns of opioid analgesics with µ opioid receptors correlate with ligand-specific voltage sensitivity (2023)
Journal Article
Kirchhofer, S. B., Lim, V. J. Y., Ernst, S., Karsai, N., Julia, R. G., Canals, M., …Bünemann, M. (2023). Differential interaction patterns of opioid analgesics with µ opioid receptors correlate with ligand-specific voltage sensitivity. eLife, 12, Article e91291. https://doi.org/10.7554/eLife.91291

The µ opioid receptor (MOR) is the key target for analgesia, but the application of opioids is accompanied by several issues. There is a wide range of opioid analgesics, differing in their chemical structure and their properties of receptor activatio... Read More about Differential interaction patterns of opioid analgesics with µ opioid receptors correlate with ligand-specific voltage sensitivity.

Synthetic yeast chromosome XI design provides a testbed for the study of extrachromosomal circular DNA dynamics (2023)
Journal Article
Blount, B. A., Lu, X., Driessen, M. R. M., Jovicevic, D., Sanchez, M. I., Ciurkot, K., …Ellis, T. (2023). Synthetic yeast chromosome XI design provides a testbed for the study of extrachromosomal circular DNA dynamics. Cell Genomics, 3(11), Article 100418. https://doi.org/10.1016/j.xgen.2023.100418

We describe construction of the synthetic yeast chromosome XI (synXI) and reveal the effects of redesign at non-coding DNA elements. The 660-kb synthetic yeast genome project (Sc2.0) chromosome was assembled from synthesized DNA fragments before CRIS... Read More about Synthetic yeast chromosome XI design provides a testbed for the study of extrachromosomal circular DNA dynamics.

Design, construction, and functional characterization of a tRNA neochromosome in yeast (2023)
Journal Article
Schindler, D., Walker, R. S., Jiang, S., Brooks, A. N., Wang, Y., Müller, C. A., …Cai, Y. (2023). Design, construction, and functional characterization of a tRNA neochromosome in yeast. Cell, 186(24), 5237-5253.e22. https://doi.org/10.1016/j.cell.2023.10.015

Here, we report the design, construction, and characterization of a tRNA neochromosome, a designer chromosome that functions as an additional, de novo counterpart to the native complement of Saccharomyces cerevisiae. Intending to address one of the c... Read More about Design, construction, and functional characterization of a tRNA neochromosome in yeast.

Modelled-Microgravity Reduces Virulence Factor Production in Staphylococcus aureus through Downregulation of agr-Dependent Quorum Sensing (2023)
Journal Article
Green, M. J., Murray, E. J., Williams, P., Ghaemmaghami, A. M., Aylott, J. W., & Williams, P. M. (2023). Modelled-Microgravity Reduces Virulence Factor Production in Staphylococcus aureus through Downregulation of agr-Dependent Quorum Sensing. International Journal of Molecular Sciences, 24(21), Article 15997. https://doi.org/10.3390/ijms242115997

Bacterial contamination during space missions is problematic for human health and damages filters and other vital support systems. Staphylococcus aureus is both a human commensal and an opportunistic pathogen that colonizes human tissues and causes a... Read More about Modelled-Microgravity Reduces Virulence Factor Production in Staphylococcus aureus through Downregulation of agr-Dependent Quorum Sensing.

The IUPHAR/BPS Guide to PHARMACOLOGY in 2024 (2023)
Journal Article
Harding, S. D., Armstrong, J. F., Faccenda, E., Southan, C., Alexander, S. H., Davenport, A. P., …Davies, J. A. (2024). The IUPHAR/BPS Guide to PHARMACOLOGY in 2024. Nucleic Acids Research, 52(D1), D1438-D1449. https://doi.org/10.1093/nar/gkad944

The IUPHAR/BPS Guide to PHARMACOLOGY (GtoPdb; https://www.guidetopharmacology.org) is an open-access, expert-curated, online database that provides succinct overviews and key references for pharmacological targets and their recommended experimental l... Read More about The IUPHAR/BPS Guide to PHARMACOLOGY in 2024.