Outputs (20)

CRISPR-Cas induced self-targeting identifies key players in archaeal microhomology mediated end joining (2025)
Journal Article
Lena Sailer, A., Wörtz, J., Smith, V., Stachler, A.-E., Blau, F., Daratha, M., Maier, L.-K., Allers, T., & Marchfelder, A. (2025). CRISPR-Cas induced self-targeting identifies key players in archaeal microhomology mediated end joining. microLife, 6, Article uqaf015. https://doi.org/10.1093/femsml/uqaf015

DNA repair processes are the foundation for genome integrity and survival, especially in extreme environments where DNA damage occurs more frequently and where archaea are found. Nevertheless, first-hand experimental information on repair pathways in... Read More about CRISPR-Cas induced self-targeting identifies key players in archaeal microhomology mediated end joining.

DNA Replication in Time and Space: The Archaeal Dimension (2025)
Journal Article
Serdyuk, A., & Allers, T. (2025). DNA Replication in Time and Space: The Archaeal Dimension. DNA, 5(2), Article 24. https://doi.org/10.3390/dna5020024

The ability of a nucleic acid molecule to self-replicate is the driving force behind the evolution of cellular life and the transition from RNA to DNA as the genetic material. Thus, the physicochemical properties of genome replication, such as the re... Read More about DNA Replication in Time and Space: The Archaeal Dimension.

HiBC: a publicly available collection of bacterial strains isolated from the human gut (2025)
Journal Article
Hitch, T. C., Masson, J. M., Pauvert, C., Bosch, J., Nüchtern, S., Treichel, N. S., Baloh, M., Razavi, S., Afrizal, A., Kousetzi, N., Aguirre, A. M., Wylensek, D., Coates, A. C., Jennings, S. A., Panyot, A., Viehof, A., Schmitz, M. A., Stuhrmann, M., Deis, E. C., Bisdorf, K., …Clavel, T. (2025). HiBC: a publicly available collection of bacterial strains isolated from the human gut. Nature Communications, 16(1), Article 4203. https://doi.org/10.1038/s41467-025-59229-9

Numerous bacteria in the human gut microbiome remain unknown and/or have yet to be cultured. While collections of human gut bacteria have been published, few strains are accessible to the scientific community. We have therefore created a publicly ava... Read More about HiBC: a publicly available collection of bacterial strains isolated from the human gut.

Genomic re-sequencing reveals mutational divergence across genetically engineered strains of model archaea (2025)
Journal Article
Soborowski, A. L., Hackley, R. K., Hwang, S., Zhou, G., Dulmage, K. A., Schönheit, P., Daniels, C., Bisson-Filho, A. W., Marchfelder, A., Maupin-Furlow, J. A., Allers, T., & Schmid, A. K. (2025). Genomic re-sequencing reveals mutational divergence across genetically engineered strains of model archaea. mSystems, 10(2), Article e01084-24. https://doi.org/10.1128/msystems.01084-24

Archaeal molecular biology has been a topic of intense research in recent decades as their role in global ecosystems, nutrient cycles, and eukaryotic evolution comes to light. The hypersaline-adapted archaeal species Halobacterium salinarum and Halof... Read More about Genomic re-sequencing reveals mutational divergence across genetically engineered strains of model archaea.

Enhanced 4-hydroxybutyrate incorporation into the PHA terpolymer of Haloferax mediterranei by heterologous expression of 4-hydroxybutyrate-CoA transferases/synthetases (2024)
Journal Article
Zheng, X., Chacón, M., Allers, T., Dixon, N., & Winterburn, J. (2024). Enhanced 4-hydroxybutyrate incorporation into the PHA terpolymer of Haloferax mediterranei by heterologous expression of 4-hydroxybutyrate-CoA transferases/synthetases. Biochemical Engineering Journal, 212, Article 109498. https://doi.org/10.1016/j.bej.2024.109498

The polyhydroxyalkanoate terpolymer, P[(3HB)-co-(3HV)-co-(4HB)], is a promising plastic alternative for specialized applications, notably in medical and pharmaceutical sectors. Haloferax mediterranei (Hfx), an extreme halophile arch... Read More about Enhanced 4-hydroxybutyrate incorporation into the PHA terpolymer of Haloferax mediterranei by heterologous expression of 4-hydroxybutyrate-CoA transferases/synthetases.

Ion-combination specific effects driving the enzymatic activity of halophilic alcohol dehydrogenase 2 from Haloferax volcanii in aqueous ionic liquid solvent mixtures (2024)
Journal Article
Schindl, A., Hagen, M. L., Cooley, I., Jäger, C. M., Warden, A. C., Zelzer, M., Allers, T., & Croft, A. K. (2024). Ion-combination specific effects driving the enzymatic activity of halophilic alcohol dehydrogenase 2 from Haloferax volcanii in aqueous ionic liquid solvent mixtures. RSC Sustainability, 2(9), 2559-2580. https://doi.org/10.1039/d3su00412k

Biocatalysis in ionic liquids enables novel routes for bioprocessing. Enzymes derived from extremophiles promise greater stability and activity under ionic liquid (IL) influence. Here, we probe the enzyme alcohol dehydrogenase 2 from the halophilic a... Read More about Ion-combination specific effects driving the enzymatic activity of halophilic alcohol dehydrogenase 2 from Haloferax volcanii in aqueous ionic liquid solvent mixtures.

A systematic analysis of affinity tags in the haloarchaeal expression system, Haloferax volcanii for protein purification (2024)
Journal Article
Karan, R., Renn, D., Allers, T., & Rueping, M. (2024). A systematic analysis of affinity tags in the haloarchaeal expression system, Haloferax volcanii for protein purification. Frontiers in Microbiology, 15, Article 1403623. https://doi.org/10.3389/fmicb.2024.1403623

Extremophilic proteins are valuable in various fields, but their expression can be challenging in traditional hosts like Escherichia coli due to misfolding and aggregation. Haloferax volcanii (H. volcanii), a halophilic expression system, offers a so... Read More about A systematic analysis of affinity tags in the haloarchaeal expression system, Haloferax volcanii for protein purification.

Archaeal Hel308 suppresses recombination through a catalytic switch that controls DNA annealing (2023)
Journal Article
Lever, R., Simmons, E., Gamble-Milner, R., Buckley, R., Harrison, C., Parkes, A., Mitchell, L., Gausden, J., Škulj, S., Bertoša, B., Bolt, E. L., & Allers, T. (2023). Archaeal Hel308 suppresses recombination through a catalytic switch that controls DNA annealing. Nucleic Acids Research, 51(16), 8563-8574. https://doi.org/10.1093/nar/gkad572

Hel308 helicases promote genome stability in archaea and are conserved in metazoans, where they are known as HELQ. Their helicase mechanism is well characterised, but it is unclear how they specifically contribute to genome stability in archaea. We s... Read More about Archaeal Hel308 suppresses recombination through a catalytic switch that controls DNA annealing.

Bioengineering of air-filled protein nanoparticles by genetic and chemical functionalization (2023)
Journal Article
Karan, R., Renn, D., Nozue, S., Zhao, L., Habuchi, S., Allers, T., & Rueping, M. (2025). Bioengineering of air-filled protein nanoparticles by genetic and chemical functionalization. Journal of Nanobiotechnology, 21(1), Article 108. https://doi.org/10.1186/s12951-023-01866-7

Background: Various bacteria and archaea, including halophilic archaeon Halobacterium sp. NRC-1 produce gas vesicle nanoparticles (GVNPs), a unique class of stable, air-filled intracellular proteinaceous nanostructures. GVNPs are an attractive tool f... Read More about Bioengineering of air-filled protein nanoparticles by genetic and chemical functionalization.

Cas1 and Fen1 Display Equivalent Functions During Archaeal DNA Repair (2022)
Journal Article
Wörtz, J., Smith, V., Fallmann, J., König, S., Thuraisingam, T., Walther, P., Urlaub, H., Stadler, P. F., Allers, T., Hille, F., & Marchfelder, A. (2022). Cas1 and Fen1 Display Equivalent Functions During Archaeal DNA Repair. Frontiers in Microbiology, 13, Article 822304. https://doi.org/10.3389/fmicb.2022.822304

CRISPR-Cas constitutes an adaptive prokaryotic defence system against invasive nucleic acids like viruses and plasmids. Beyond their role in immunity, CRISPR-Cas systems have been shown to closely interact with components of cellular DNA repair pathw... Read More about Cas1 and Fen1 Display Equivalent Functions During Archaeal DNA Repair.