Abida Naemat
Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman spectroscopy
Naemat, Abida; Elsheikha, Hany M.; Al-sandaqchib, Alaa; Kong, Kenny; Ghita, Adrian; Notingher, Ioan
Authors
Professor HANY ELSHEIKHA hany.elsheikha@nottingham.ac.uk
Professor of Interdisciplinary Parasitology
Alaa Al-sandaqchib
Kenny Kong
Adrian Ghita
IOAN NOTINGHER IOAN.NOTINGHER@NOTTINGHAM.AC.UK
Professor of Physics
Abstract
Label-free imaging using Raman micro-spectroscopy (RMS) was used to characterize the spatio-temporal molecular changes of T. gondii tachyzoites and their host cell microenvironment. Raman spectral maps were recorded from isolated T. gondii tachyzoites and T. gondii-infected human retinal cells at 6 h, 24 h and 48 h post-infection. Principal component analysis (PCA) of the Raman spectra of paraformaldehyde-fixed infected cells indicated a significant increase in the amount of lipids and proteins in the T. gondii tachyzoites as the infection progresses within host cells. These results were confirmed by experiments carried out on live T. gondii-infected cells and were correlated with an increase in the concentration of proteins and lipids required for the replication of this intracellular pathogen. These findings demonstrate the potential of RMS to characterize time- and spatially-dependent molecular interactions between intracellular pathogens and the host cells. Such information may be useful for discovery of pharmacological targets or screening compounds with potential neuro-protective activity for eminent effects of changes in brain infection control practices.
Citation
Naemat, A., Elsheikha, H. M., Al-sandaqchib, A., Kong, K., Ghita, A., & Notingher, I. (2015). Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman spectroscopy. Analyst, 140(3), 756-764. https://doi.org/10.1039/c4an01810a
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 19, 2014 |
Online Publication Date | Nov 19, 2014 |
Publication Date | Feb 7, 2015 |
Deposit Date | Sep 2, 2015 |
Publicly Available Date | Sep 2, 2015 |
Journal | The Analyst |
Print ISSN | 0003-2654 |
Electronic ISSN | 1364-5528 |
Publisher | Royal Society of Chemistry |
Peer Reviewed | Peer Reviewed |
Volume | 140 |
Issue | 3 |
Pages | 756-764 |
DOI | https://doi.org/10.1039/c4an01810a |
Public URL | https://nottingham-repository.worktribe.com/output/739427 |
Publisher URL | http://pubs.rsc.org/en/content/articlelanding/2015/an/c4an01810a#!divAbstract |
Contract Date | Sep 2, 2015 |
Files
Naemat et al 2015_OA.pdf
(10.6 Mb)
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