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Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman spectroscopy

Naemat, Abida; Elsheikha, Hany M.; Al-sandaqchib, Alaa; Kong, Kenny; Ghita, Adrian; Notingher, Ioan

Authors

Abida Naemat

Hany M. Elsheikha

Alaa Al-sandaqchib

Adrian Ghita

Ioan Notingher ioan.notingher@nottingham.ac.uk



Abstract

Label-free imaging using Raman micro-spectroscopy (RMS) was used to characterize the spatio-temporal molecular changes of T. gondii tachyzoites and their host cell microenvironment. Raman spectral maps were recorded from isolated T. gondii tachyzoites and T. gondii-infected human retinal cells at 6 h, 24 h and 48 h post-infection. Principal component analysis (PCA) of the Raman spectra of paraformaldehyde-fixed infected cells indicated a significant increase in the amount of lipids and proteins in the T. gondii tachyzoites as the infection progresses within host cells. These results were confirmed by experiments carried out on live T. gondii-infected cells and were correlated with an increase in the concentration of proteins and lipids required for the replication of this intracellular pathogen. These findings demonstrate the potential of RMS to characterize time- and spatially-dependent molecular interactions between intracellular pathogens and the host cells. Such information may be useful for discovery of pharmacological targets or screening compounds with potential neuro-protective activity for eminent effects of changes in brain infection control practices.

Journal Article Type Article
Publication Date Feb 7, 2015
Journal The Analyst
Print ISSN 0003-2654
Electronic ISSN 1364-5528
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 140
Issue 3
Pages 756-764
APA6 Citation Naemat, A., Elsheikha, H. M., Al-sandaqchib, A., Kong, K., Ghita, A., & Notingher, I. (2015). Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman spectroscopy. Analyst, 140(3), 756-764. https://doi.org/10.1039/c4an01810a
DOI https://doi.org/10.1039/c4an01810a
Publisher URL http://pubs.rsc.org/en/content/articlelanding/2015/an/c4an01810a#!divAbstract
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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