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Modulation of the pre-metastatic bone niche: molecular changes mediated by bone-homing prostate cancer extracellular vesicles (2024)
Journal Article
Brown, T. J., Rutland, C. S., Rutland, C. S., Choi, K. K., Tse, F., Pfeffers, M. J., …James, V. (2024). Modulation of the pre-metastatic bone niche: molecular changes mediated by bone-homing prostate cancer extracellular vesicles. Frontiers in Cell and Developmental Biology, 12, Article 1354606. https://doi.org/10.3389/fcell.2024.1354606

Prostate cancer (PCa) is a leading male malignancy worldwide, often progressing to bone metastasis, with limited curative options. Extracellular vesicles (EVs) have emerged as key players in cancer communication and metastasis, promoting the formatio... Read More about Modulation of the pre-metastatic bone niche: molecular changes mediated by bone-homing prostate cancer extracellular vesicles.

Multi-Omic Temporal Landscape of Plasma and Synovial Fluid-Derived Extracellular Vesicles Using an Experimental Model of Equine Osteoarthritis (2023)
Journal Article
Anderson, J. R., Johnson, E., Jenkins, R., Jacobsen, S., Green, D., Walters, M., …Peffers, M. J. (2023). Multi-Omic Temporal Landscape of Plasma and Synovial Fluid-Derived Extracellular Vesicles Using an Experimental Model of Equine Osteoarthritis. International Journal of Molecular Sciences, 24(19), Article 14888. https://doi.org/10.3390/ijms241914888

Extracellular vesicles (EVs) contribute to osteoarthritis pathogenesis through their release into joint tissues and synovial fluid. Synovial fluid-derived EVs have the potential to be direct biomarkers in the causal pathway of disease but also enable... Read More about Multi-Omic Temporal Landscape of Plasma and Synovial Fluid-Derived Extracellular Vesicles Using an Experimental Model of Equine Osteoarthritis.

Small non-coding RNA landscape of extracellular vesicles from an experimental model of equine osteoarthritis (2023)
Journal Article
Peffers, M., Anderson, J., Jacobsen, S., Walters, M., Bundgaard, L., Hackle, M., & James, V. (2023). Small non-coding RNA landscape of extracellular vesicles from an experimental model of equine osteoarthritis. Orthopaedic Proceedings, 105-B(SUPP_8), 119-119. https://doi.org/10.1302/1358-992x.2023.8.119

Joint tissues release extracellular vesicles (EVs) that potentially sustain joint homeostasis and contribute to osteoarthritis (OA) pathogenesis. EVs are putative novel therapeutics for OA, and transport biologically active molecules (including small... Read More about Small non-coding RNA landscape of extracellular vesicles from an experimental model of equine osteoarthritis.

PBMC-derived extracellular vesicles in a smoking-related inflammatory disease model (2023)
Journal Article
Gomez, N. E., James, V., Arkill, K. P., Nizamudeen, Z. A., Onion, D., & Fairclough, L. C. (2023). PBMC-derived extracellular vesicles in a smoking-related inflammatory disease model. European Journal of Immunology, 53(6), Article 2250143. https://doi.org/10.1002/eji.202250143

Extracellular vesicles (EVs) function as mediators of intercellular communication and as such influence the recipient cell function. EVs derived from immune cells can carry out many of the same functions as their parental cells, as they carry costimu... Read More about PBMC-derived extracellular vesicles in a smoking-related inflammatory disease model.

Clinical and molecular significance of the RNA m6A methyltransferase complex in prostate cancer (2023)
Journal Article
Lothion-Roy, J., Haigh, D. B., Harris, A. E., Metzler, V. M., Alsaleem, M., Toss, M. S., …Woodcock, C. L. (2023). Clinical and molecular significance of the RNA m6A methyltransferase complex in prostate cancer. Frontiers in Genetics, 13, Article 1096071. https://doi.org/10.3389/fgene.2022.1096071

N6-methyladenosine (m6A) is the most abundant internal mRNA modification and is dynamically regulated through distinct protein complexes that methylate, demethylate, and/or interpret the m6A modification. These proteins, and the m6A modification, are... Read More about Clinical and molecular significance of the RNA m6A methyltransferase complex in prostate cancer.

Temporal extracellular vesicle protein changes following intraarticular treatment with integrin α10β1-selected mesenchymal stem cells in equine osteoarthritis (2022)
Journal Article
Clarke, E. J., Johnson, E., Caamaño Gutierrez, E., Andersen, C., Berg, L. C., Jenkins, R. E., …Peffers, M. J. (2022). Temporal extracellular vesicle protein changes following intraarticular treatment with integrin α10β1-selected mesenchymal stem cells in equine osteoarthritis. Frontiers in Veterinary Science, 9, Article 1057667. https://doi.org/10.3389/fvets.2022.1057667

Introduction: Equine osteoarthritis (OA) is a heterogeneous, degenerative disease of the musculoskeletal system with multifactorial causation, characterized by a joint metabolic imbalance. Extracellular vesicles are nanoparticles involved in intracel... Read More about Temporal extracellular vesicle protein changes following intraarticular treatment with integrin α10β1-selected mesenchymal stem cells in equine osteoarthritis.

Exploring anti-androgen therapies in hormone dependent prostate cancer and new therapeutic routes for castration resistant prostate cancer (2022)
Journal Article
Harris, A. E., Metzler, V. M., Lothion-Roy, J., Varun, D., Woodcock, C. L., Haigh, D. B., …Jeyapalan, J. N. (2022). Exploring anti-androgen therapies in hormone dependent prostate cancer and new therapeutic routes for castration resistant prostate cancer. Frontiers in Endocrinology, 13, Article 1006101. https://doi.org/10.3389/fendo.2022.1006101

Androgen deprivation therapies (ADTs) are important treatments which inhibit androgen-induced prostate cancer (PCa) progression by either preventing androgen biosynthesis (e.g. abiraterone) or by antagonizing androgen receptor (AR) function (e.g. bic... Read More about Exploring anti-androgen therapies in hormone dependent prostate cancer and new therapeutic routes for castration resistant prostate cancer.

The role of extracellular vesicle miRNAs and tRNAs in synovial fibroblast senescence (2022)
Journal Article
Wijesinghe, S. N., Anderson, J., Brown, T. J., Nanus, D. E., Housmans, B., Green, J. A., …Peffers, M. J. (2022). The role of extracellular vesicle miRNAs and tRNAs in synovial fibroblast senescence. Frontiers in Molecular Biosciences, 9, Article 971621. https://doi.org/10.3389/fmolb.2022.971621

Extracellular vesicles are mediators of intercellular communication with critical roles in cellular senescence and ageing. In arthritis, senescence is linked to the activation of a pro-inflammatory phenotype contributing to chronic arthritis pathogen... Read More about The role of extracellular vesicle miRNAs and tRNAs in synovial fibroblast senescence.

Optical photothermal infrared spectroscopy can differentiate equine osteoarthritic plasma extracellular vesicles from healthy controls (2022)
Journal Article
Clarke, E. J., Lima, C., Anderson, J. R., Castanheira, C., Beckett, A., James, V., …Peffers, M. J. (2022). Optical photothermal infrared spectroscopy can differentiate equine osteoarthritic plasma extracellular vesicles from healthy controls. Analytical Methods, 14(37), 3661-3670. https://doi.org/10.1039/d2ay00779g

Equine osteoarthritis is a chronic degenerative disease of the articular joint, characterised by cartilage degradation resulting in pain and reduced mobility and thus is a prominent equine welfare concern. Diagnosis is usually at a late stage through... Read More about Optical photothermal infrared spectroscopy can differentiate equine osteoarthritic plasma extracellular vesicles from healthy controls.

Small non-coding RNA landscape of extracellular vesicles from a post-traumatic model of equine osteoarthritis (2022)
Journal Article
Anderson, J. R., Jacobsen, S., Walters, M., Bundgaard, L., Diendorfer, A., Hackl, M., …Peffers, M. J. (2022). Small non-coding RNA landscape of extracellular vesicles from a post-traumatic model of equine osteoarthritis. Frontiers in Veterinary Science, 9, Article 901269. https://doi.org/10.3389/fvets.2022.901269

Extracellular vesicles comprise an as yet inadequately investigated intercellular communication pathway in the field of early osteoarthritis. We hypothesised that the small non-coding RNA expression pattern in synovial fluid and plasma would change d... Read More about Small non-coding RNA landscape of extracellular vesicles from a post-traumatic model of equine osteoarthritis.

Impact of COVID-19 on student attainment and pedagogical needs when undertaking independent scientific research (2022)
Journal Article
Jeyapalan, J. N., James, V., Gardner, D. S., Lothion-Roy, J. H., Mongan, N. P., & Rutland, C. S. (2022). Impact of COVID-19 on student attainment and pedagogical needs when undertaking independent scientific research. Anatomia, Histologia, Embryologia, https://doi.org/10.1111/ahe.12842

Research is often an essential component of completing a veterinary medicine degree, with universities worldwide aiming to teach students a variety of techniques and general research comprehension and skills. As universities worldwide navigated the C... Read More about Impact of COVID-19 on student attainment and pedagogical needs when undertaking independent scientific research.

Epithelial to mesenchymal transition influences fibroblast phenotype in colorectal cancer by altering miR-200 levels in extracellular vesicles (2022)
Journal Article
Bhome, R., Emaduddin, M., James, V., House, L. M., Thirdborough, S. M., Mellone, M., …Sayan, A. E. (2022). Epithelial to mesenchymal transition influences fibroblast phenotype in colorectal cancer by altering miR-200 levels in extracellular vesicles. Journal of Extracellular Vesicles, 11(5), Article e12226. https://doi.org/10.1002/jev2.12226

Colorectal cancer (CRC) with a mesenchymal gene expression signature has the greatest propensity for distant metastasis and is characterised by the accumulation of cancer-associated fibroblasts in the stroma. We investigated whether the epithelial to... Read More about Epithelial to mesenchymal transition influences fibroblast phenotype in colorectal cancer by altering miR-200 levels in extracellular vesicles.

Extracellular Vesicles Derived from Mesenchymal Stem Cells: Biological Activity and Approaches of Large-Scale Production (2022)
Book Chapter
Gomzikova, M. O., James, V., & Rizvanov, A. A. (2022). Extracellular Vesicles Derived from Mesenchymal Stem Cells: Biological Activity and Approaches of Large-Scale Production. In K. H. Haider (Ed.), Handbook of stem cell therapy. Springer Singapore. https://doi.org/10.1007/978-981-16-6016-0_48-1

The discovery of extracellular vesicles has provided an outstanding breakthrough in stem cells and regenerative medicine. It has been shown that cells can transfer information through the secretion of soluble factors, the formation of direct physical... Read More about Extracellular Vesicles Derived from Mesenchymal Stem Cells: Biological Activity and Approaches of Large-Scale Production.

Extracellular vesicles and chronic obstructive pulmonary disease (COPD): a systematic review (2022)
Journal Article
Gomez, N., James, V., Onion, D., & Fairclough, L. C. (2022). Extracellular vesicles and chronic obstructive pulmonary disease (COPD): a systematic review. Respiratory Research, 23, Article 82. https://doi.org/10.1186/s12931-022-01984-0

Background: Chronic Obstructive Pulmonary Disease (COPD) is a common inflammatory disease of the airways characterized by irreversible airflow limitation, ranking the third highest cause of death worldwide. Extracellular vesicles (EVs) are important... Read More about Extracellular vesicles and chronic obstructive pulmonary disease (COPD): a systematic review.

Increasing Severity of Spinal Cord Injury Results in Microglia/Macrophages With Annular-Shaped Morphology and No Change in Expression of CD40 and Tumor Growth Factor-β During the Chronic Post-injury Stage (2022)
Journal Article
Akhmetzyanova, E. R., Timofeeva, A. V., Sabirov, D. K., Kostennikov, A. A., Rogozhin, A. A., James, V., …Mukhamedshina, Y. O. (2022). Increasing Severity of Spinal Cord Injury Results in Microglia/Macrophages With Annular-Shaped Morphology and No Change in Expression of CD40 and Tumor Growth Factor-β During the Chronic Post-injury Stage. Frontiers in Molecular Neuroscience, 14, Article 802558. https://doi.org/10.3389/fnmol.2021.802558

Determination of the quantitative composition of phenotypically and morphologically different populations of resident microglia and infiltrating macrophages in spinal cord injury (SCI) of various degrees of severity could lead to much needed novel th... Read More about Increasing Severity of Spinal Cord Injury Results in Microglia/Macrophages With Annular-Shaped Morphology and No Change in Expression of CD40 and Tumor Growth Factor-β During the Chronic Post-injury Stage.

Cellular and Molecular Gradients in the Ventral Horns With Increasing Distance From the Injury Site After Spinal Cord Contusion (2022)
Journal Article
Kabdesh, I. M., Mukhamedshina, Y. O., Arkhipova, S. S., Sabirov, D. K., Kuznecov, M. S., Vyshtakalyuk, A. B., …Chelyshev, Y. A. (2022). Cellular and Molecular Gradients in the Ventral Horns With Increasing Distance From the Injury Site After Spinal Cord Contusion. Frontiers in Cellular Neuroscience, 16, Article 817752. https://doi.org/10.3389/fncel.2022.817752

To identify cellular and molecular gradients following spinal cord injury (SCI), a rat contusion model of severe SCI was used to investigate the expression of NG2 and molecules that identify astrocytes and axons of the ventral horns (VH) at different... Read More about Cellular and Molecular Gradients in the Ventral Horns With Increasing Distance From the Injury Site After Spinal Cord Contusion.

The Study of Cerebrospinal Fluid microRNAs in Spinal Cord Injury and Neurodegenerative Diseases: Methodological Problems and Possible Solutions (2021)
Journal Article
Baichurina, I., Valiullin, V., James, V., Rizvanov, A., & Mukhamedshina, Y. (2021). The Study of Cerebrospinal Fluid microRNAs in Spinal Cord Injury and Neurodegenerative Diseases: Methodological Problems and Possible Solutions. International Journal of Molecular Sciences, 23(1), Article 114. https://doi.org/10.3390/ijms23010114

Despite extensive research on neurological disorders, unanswered questions remain regarding the molecular mechanisms underpinning the course of these diseases, and the search continues for effective biomarkers for early diagnosis, prognosis, or thera... Read More about The Study of Cerebrospinal Fluid microRNAs in Spinal Cord Injury and Neurodegenerative Diseases: Methodological Problems and Possible Solutions.

Distinct small non-coding RNA landscape in the axons and released extracellular vesicles of developing primary cortical neurons and the axoplasm of adult nerves (2021)
Journal Article
Mesquita-Ribeiro, R., Fort, R. S., Rathbone, A., Farias, J., Lucci, C., James, V., …Dajas-Bailador, F. (2021). Distinct small non-coding RNA landscape in the axons and released extracellular vesicles of developing primary cortical neurons and the axoplasm of adult nerves. RNA Biology, 18(sup2), 832-855. https://doi.org/10.1080/15476286.2021.2000792

Neurons have highlighted the needs for decentralized gene expression and specific RNA function in somato-dendritic and axonal compartments, as well as in intercellular communication via extracellular vesicles (EVs). Despite advances in miRNA biology,... Read More about Distinct small non-coding RNA landscape in the axons and released extracellular vesicles of developing primary cortical neurons and the axoplasm of adult nerves.

Transcriptomic Analysis of Cardiomyocyte Extracellular Vesicles in Hypertrophic Cardiomyopathy Reveals Differential snoRNA Cargo (2021)
Journal Article
James, V., Nizamudeen, Z. A., Lea, D., Dottorini, T., Holmes, T. L., Johnson, B. B., …Smith, J. G. (2021). Transcriptomic Analysis of Cardiomyocyte Extracellular Vesicles in Hypertrophic Cardiomyopathy Reveals Differential snoRNA Cargo. Stem Cells and Development, 30(24), 1215-1227. https://doi.org/10.1089/scd.2021.0202

Hypertrophic cardiomyopathy (HCM) is characterised by increased left ventricular wall thickness that can lead to devastating conditions such as heart failure and sudden cardiac death. Despite extensive study, the mechanisms mediating many of the asso... Read More about Transcriptomic Analysis of Cardiomyocyte Extracellular Vesicles in Hypertrophic Cardiomyopathy Reveals Differential snoRNA Cargo.

Comparative pathology of dog and human prostate cancer (2021)
Journal Article
Ryman-Tubb, T., Lothion-Roy, J. H., Metzler, V. M., Harris, A. E., Robinson, B. D., Rizvanov, A. A., …de Brot, S. (2022). Comparative pathology of dog and human prostate cancer. Veterinary Medicine and Science, 8(1), 110-120. https://doi.org/10.1002/vms3.642

Though relatively rare in dogs, prostate cancer (PCa) is the most common non-cutaneous cancer in men. Human and canine prostate glands share many functional, anatomical and physiological features. Due to these similarities, canine PCa has been propos... Read More about Comparative pathology of dog and human prostate cancer.

Extracellular vesicles, tumor growth, and the metastatic process (2021)
Book Chapter
Wesson, F., Brown, T. J., & James, V. (2022). Extracellular vesicles, tumor growth, and the metastatic process. In D. Heymann (Ed.), Bone Cancer: Bone Sarcomas and Bone Metastases - From Bench to Bedside (275-284). (3rd). Elsevier. https://doi.org/10.1016/B978-0-12-821666-8.00058-X

Extracellular vesicles (EVs) form an important part of cell-to-cell communication, with roles in both health and disease. Within the cancer setting, this heterogenous population of extracellular messengers can have both pro- and antitumorigenic prope... Read More about Extracellular vesicles, tumor growth, and the metastatic process.

Extracellular Vesicles and Chronic Obstructive Pulmonary Disease (COPD): A Systematic Review (2021)
Working Paper
Gomez, N., James, V., Onion, D., & Fairclough, L. Extracellular Vesicles and Chronic Obstructive Pulmonary Disease (COPD): A Systematic Review

Background: Chronic Obstructive Pulmonary Disease (COPD) is a common inflammatory disease of the airways characterized by irreversible airflow limitation, ranking the third highest cause of death worldwide. Extracellular vesicles (EVs) are important... Read More about Extracellular Vesicles and Chronic Obstructive Pulmonary Disease (COPD): A Systematic Review.

The Role of Extracellular Vesicles in the Development of a Cancer Stem Cell Microenvironment Niche and Potential Therapeutic Targets: a systematic review (2021)
Journal Article
Brown, T. J., & James, V. (2021). The Role of Extracellular Vesicles in the Development of a Cancer Stem Cell Microenvironment Niche and Potential Therapeutic Targets: a systematic review. Cancers, 13(10), Article 2435. https://doi.org/10.3390/cancers13102435

Cancer stem cells (CSCs) have increasingly been shown to be a crucial element of heterogenous tumors. Although a relatively small component of the population, they increase the resistance to treatment and the likelihood of recurrence. In recent years... Read More about The Role of Extracellular Vesicles in the Development of a Cancer Stem Cell Microenvironment Niche and Potential Therapeutic Targets: a systematic review.

Storage stability and delivery potential of cytochalasin B induced membrane vesicles (2021)
Journal Article
Kletukhina, S. K., Neustroeva, O. A., Kurbangaleeva, S. V., Salafutdinov, I. I., Rogov, A. M., James, V., …Gomzikova, M. O. (2021). Storage stability and delivery potential of cytochalasin B induced membrane vesicles. Biotechnology Reports, 30, Article e00616. https://doi.org/10.1016/j.btre.2021.e00616

Cell-free therapies based on extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) are considered as a promising tool for stimulating regeneration and immunomodulation. The need to develop a practical approach for large-scale produc... Read More about Storage stability and delivery potential of cytochalasin B induced membrane vesicles.

Mitochondria donation by mesenchymal stem cells: current understanding and mitochondria transplantation strategies (2021)
Journal Article
Gomzikova, M., James, V., & Rizvanov, A. (2021). Mitochondria donation by mesenchymal stem cells: current understanding and mitochondria transplantation strategies. Frontiers in Cell and Developmental Biology, 9, Article 653322. https://doi.org/10.3389/fcell.2021.653322

The phenomenon of mitochondria donation is found in various tissues of humans and animals and is attracting increasing attention. To date, numerous studies have described the transfer of mitochondria from stem cells to injured cells, leading to incre... Read More about Mitochondria donation by mesenchymal stem cells: current understanding and mitochondria transplantation strategies.

Blood Serum Cytokines in Patients with Subacute Spinal Cord Injury: A Pilot Study to Search for Biomarkers of Injury Severity (2021)
Journal Article
Ogurcov, S., Shulman, I., Garanina, E., Sabirov, D., Baichurina, I., Kuznetcov, M., …Mukhamedshina, Y. (2021). Blood Serum Cytokines in Patients with Subacute Spinal Cord Injury: A Pilot Study to Search for Biomarkers of Injury Severity. Brain Sciences, 11(3), Article 322. https://doi.org/10.3390/brainsci11030322

Background: Despite considerable interest in the search for a spinal cord injury (SCI) therapy, there is a critical need to develop a panel of diagnostic biomarkers to determine injury severity. In this regard, there is a requirement for continuing r... Read More about Blood Serum Cytokines in Patients with Subacute Spinal Cord Injury: A Pilot Study to Search for Biomarkers of Injury Severity.

Cytochalasin B-Induced Membrane Vesicles from Human Mesenchymal Stem Cells Overexpressing IL2 Are Able to Stimulate CD8+ T-Killers to Kill Human Triple Negative Breast Cancer Cells (2021)
Journal Article
Chulpanova, D. S., Gilazieva, Z. E., Kletukhina, S. K., Aimaletdinov, A. M., Garanina, E. E., James, V., …Solovyeva, V. V. (2021). Cytochalasin B-Induced Membrane Vesicles from Human Mesenchymal Stem Cells Overexpressing IL2 Are Able to Stimulate CD8+ T-Killers to Kill Human Triple Negative Breast Cancer Cells. Biology, 10(2), Article 141. https://doi.org/10.3390/biology10020141

Interleukin 2 (IL2) was one of the first cytokines used for cancer treatment due to its ability to stimulate anti-cancer immunity. However, recombinant IL2-based therapy is associated with high systemic toxicity and activation of regulatory T-cells,... Read More about Cytochalasin B-Induced Membrane Vesicles from Human Mesenchymal Stem Cells Overexpressing IL2 Are Able to Stimulate CD8+ T-Killers to Kill Human Triple Negative Breast Cancer Cells.

Human Mesenchymal Stem Cells Overexpressing Interleukin 2 Can Suppress Proliferation of Neuroblastoma Cells in Co-Culture and Activate Mononuclear Cells In Vitro (2020)
Journal Article
Chulpanova, D. S., Solovyeva, V. V., James, V., Arkhipova, S. S., Gomzikova, M. O., Garanina, E. E., …Rizvanov, A. A. (2020). Human Mesenchymal Stem Cells Overexpressing Interleukin 2 Can Suppress Proliferation of Neuroblastoma Cells in Co-Culture and Activate Mononuclear Cells In Vitro. Bioengineering, 7(2), Article 59. https://doi.org/10.3390/bioengineering7020059

High-dose recombinant interleukin 2 (IL2) therapy has been shown to be successful in renal cell carcinoma and metastatic melanoma. However, systemic administration of high doses of IL2 can be toxic, causing capillary leakage syndrome and stimulating... Read More about Human Mesenchymal Stem Cells Overexpressing Interleukin 2 Can Suppress Proliferation of Neuroblastoma Cells in Co-Culture and Activate Mononuclear Cells In Vitro.

Mesenchymal Stem Cell Therapy for Spinal Cord Contusion: A Comparative Study on Small and Large Animal Models (2019)
Journal Article
Mukhamedshina, Y., Shulman, I., Ogurcov, S., Kostennikov, A., Zakirova, E., Akhmetzyanova, E., …Rizvanov, A. (2019). Mesenchymal Stem Cell Therapy for Spinal Cord Contusion: A Comparative Study on Small and Large Animal Models. Biomolecules, 9(12), Article 811. https://doi.org/10.3390/biom9120811

Here, we provide a first comparative study of the therapeutic potential of allogeneic mesenchymal stem cells derived from bone marrow (BM-MSCs), adipose tissue (AD-MSCs), and dental pulp (DP-MSCs) embedded in fibrin matrix, in small (rat) and large (... Read More about Mesenchymal Stem Cell Therapy for Spinal Cord Contusion: A Comparative Study on Small and Large Animal Models.

Therapeutic Application of Mesenchymal Stem Cells Derived Extracellular Vesicles for Immunomodulation (2019)
Journal Article
Gomzikova, M. O., James, V., & Rizvanov, A. A. (2019). Therapeutic Application of Mesenchymal Stem Cells Derived Extracellular Vesicles for Immunomodulation. Frontiers in Immunology, 10, https://doi.org/10.3389/fimmu.2019.02663

The immunosuppressive potential of mesenchymal stem cells has been extensively investigated in many studies in vivo and in vitro. In recent years, a variety preclinical and clinical studies have demonstrated that mesenchymal stem cells ameliorate imm... Read More about Therapeutic Application of Mesenchymal Stem Cells Derived Extracellular Vesicles for Immunomodulation.

Specific trophoblast transcripts transferred by extracellular vesicles affect gene expression in endometrial epithelial cells and may have a role in embryo-maternal crosstalk (2019)
Journal Article
Es-Haghi, M., Godakumara, K., Häling, A., Lättekivi, F., Lavrits, A., Viil, J., …Fazeli, A. (2019). Specific trophoblast transcripts transferred by extracellular vesicles affect gene expression in endometrial epithelial cells and may have a role in embryo-maternal crosstalk. Cell Communication and Signaling, 17(1), Article 146. https://doi.org/10.1186/s12964-019-0448-x

© 2019 The Author(s). Background: Successful establishment of pregnancy hinges on appropriate communication between the embryo and the uterus prior to implantation, but the nature of this communication remains poorly understood. Here, we tested the h... Read More about Specific trophoblast transcripts transferred by extracellular vesicles affect gene expression in endometrial epithelial cells and may have a role in embryo-maternal crosstalk.

Extracellular vesicles in the diagnosis and treatment of central nervous system diseases (2019)
Journal Article
Shaimardanova, A. A., Solovyeva, V. V., Chulpanova, D. S., James, V., Kitaeva, K. V., & Rizvanov, A. A. (2020). Extracellular vesicles in the diagnosis and treatment of central nervous system diseases. Neural Regeneration Research, 15(4), 586=596. https://doi.org/10.4103/1673-5374.266908

Extracellular vesicles, exosomes and microvesicles, play a fundamental role in the activity of the nervous system, participating in signal transmission between neurons and providing the interaction of central nervous system (CNS) with all body system... Read More about Extracellular vesicles in the diagnosis and treatment of central nervous system diseases.

Role of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Epithelial-Mesenchymal Transition (2019)
Journal Article
Kletukhina, S., Neustroeva, O., James, V., Rizvanov, A., & Gomzikova, M. (2019). Role of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Epithelial-Mesenchymal Transition. International Journal of Molecular Sciences, 20(19), https://doi.org/10.3390/ijms20194813

Epithelial-mesenchymal transition (EMT) is a process that takes place during embryonic development, wound healing, and under some pathological processes, including fibrosis and tumor progression. The molecular changes occurring within epithelial cell... Read More about Role of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Epithelial-Mesenchymal Transition.

Analysis of the interaction and proliferative activity of adenocarcinoma, peripheral blood mononuclear and mesenchymal stromal cells after co-cultivation in vitro (2019)
Journal Article
Kitaeva, K. V., Prudnikov, T. S., Gomzikova, M. O., Kletukhina, S. K., James, V., Rizvanov, A. A., & Solovyeva, V. V. (2019). Analysis of the interaction and proliferative activity of adenocarcinoma, peripheral blood mononuclear and mesenchymal stromal cells after co-cultivation in vitro. BioNanoScience, 9(2), 502-509. https://doi.org/10.1007/s12668-019-00625-z

The tumor microenvironment is a heterogeneous population of cells actively involved in the process of growth and development of a tumor. Research has demonstrated the interactions between the different populations of cells are critical for the format... Read More about Analysis of the interaction and proliferative activity of adenocarcinoma, peripheral blood mononuclear and mesenchymal stromal cells after co-cultivation in vitro.

Therapeutic Potential of Extracellular Vesicles for the Treatment of Nerve Disorders (2019)
Journal Article
Galieva, L. R., James, V., Mukhamedshina, Y. O., & Rizvanov, A. A. (2019). Therapeutic Potential of Extracellular Vesicles for the Treatment of Nerve Disorders. Frontiers in Neuroscience, 13, Article 163. https://doi.org/10.3389/fnins.2019.00163

The use of extracellular vesicles (EVs) as cell free therapy is a promising approach to stimulate tissue regeneration including that of the nervous system. EVs transfer bioactive proteins and lipids, RNA and microRNAs, which play a relevant role in E... Read More about Therapeutic Potential of Extracellular Vesicles for the Treatment of Nerve Disorders.

Communication of prostate cancer cells with bone cells via extracellular vesicle RNA; a potential mechanism of metastasis (2018)
Journal Article
Probert, C., Dottorini, T., Speakman, A., Hunt, S., Nafee, T., Fazeli, A., …James, V. (2019). Communication of prostate cancer cells with bone cells via extracellular vesicle RNA; a potential mechanism of metastasis. Oncogene, 38(10), 1751-1763. https://doi.org/10.1038/s41388-018-0540-5

© 2018, The Author(s). The role of extracellular vesicles (EVs) as vehicles for cell-to-cell communication between a tumour and its environment is a relatively new concept. The hypothesis that EVs may be critical in co-opting tissues by tumours to ge... Read More about Communication of prostate cancer cells with bone cells via extracellular vesicle RNA; a potential mechanism of metastasis.

Therapeutic prospects of extracellular vesicles in cancer treatment (2018)
Journal Article
Chulpanoval, D. S., Kitaeva, K. V., James, V., Rizvanov, A. A., & Solovyeval, V. V. (2018). Therapeutic prospects of extracellular vesicles in cancer treatment. Frontiers in Immunology, 9, Article 1534. https://doi.org/10.3389/fimmu.2018.01534

Extracellular vesicles (EVs) are released by all cells within the tumor microenvironment, such as endothelial cells, tumor-associated fibroblasts, pericytes and immune system cells. The EVs carry the cargo of parental cells formed of proteins and nuc... Read More about Therapeutic prospects of extracellular vesicles in cancer treatment.

Application of mesenchymal stem cells for therapeutic agent delivery in anti-tumor treatment (2018)
Journal Article
Chulpanoval, D. S., Kitaeva, K. V., Tazetdinoval, L. G., James, V., Rizvanov, A. A., & Solovyeval, V. V. (2018). Application of mesenchymal stem cells for therapeutic agent delivery in anti-tumor treatment. Frontiers in Pharmacology, 9, Article 259. https://doi.org/10.3389/fphar.2018.00259

Mesenchymal stem cells (MSCs) are non-hematopoietic progenitor cells, which can be isolated from different types of tissues including bone marrow, adipose tissue, tooth pulp, and placenta/umbilical cord blood. There isolation from adult tissues circu... Read More about Application of mesenchymal stem cells for therapeutic agent delivery in anti-tumor treatment.

Epigenetic control of microRNA expression and cancer (2017)
Book Chapter
De Brot, S., Rutland, C. S., Mongan, N. P., & James, V. (2018). Epigenetic control of microRNA expression and cancer. In J. Chakrabarti, & S. Mitra (Eds.), Cancer and Noncoding RNAs, 373-380. Elsevier. doi:10.1016/B978-0-12-811022-5.00020-6

MicroRNAs (miRNAs or miRs) are involved in pathways central to homeostasis and cellular function, including proliferation, differentiation, and apoptosis; processes which when aberrantly regulated contribute to the hallmarks of cancer. The contributi... Read More about Epigenetic control of microRNA expression and cancer.

Heterochromatin modulation and PCG control of gene expression mediated by noncoding RNA in cancer (2017)
Book Chapter
Rutland, C. S., de Brot, S., James, V., & Mongan, N. P. (2018). Heterochromatin modulation and PCG control of gene expression mediated by noncoding RNA in cancer. In J. Chakrabarti, & S. Mitra (Eds.), Cancer and Noncoding RNAs, 359-372. Elsevier. doi:10.1016/b978-0-12-811022-5.00019-x

In eukaryotes, the packaging of DNA and proteins, termed chromatin, is an essential determinant of transcription. The basic unit of chromatin, the nucleosome, is composed of ?146 base pairs of DNA spooled around a dense protein core containing two co... Read More about Heterochromatin modulation and PCG control of gene expression mediated by noncoding RNA in cancer.

Regulation of vascular endothelial growth factor in prostate cancer (2015)
Journal Article
de Brot, S., Ntekim, A., Cardenas, R., James, V., Allegrucci, C., Heery, D. M., …Mongan, N. P. (2015). Regulation of vascular endothelial growth factor in prostate cancer. Endocrine-Related Cancer, 22(3), Article R107-R123. https://doi.org/10.1530/ERC-15-0123

Prostate cancer (PCa) is the most common malignancy affecting men in the western world. Although radical prostatectomy and radiation therapy can successfully treat PCa in the majority of patients, up to ?30% will experience local recurrence or metast... Read More about Regulation of vascular endothelial growth factor in prostate cancer.

Circulating microRNAs for the prediction of metastasis in breast cancer patients diagnosed with early stage disease (2015)
Journal Article
Inns, J., & James, V. (2015). Circulating microRNAs for the prediction of metastasis in breast cancer patients diagnosed with early stage disease. Breast, 24(4), 364-369. https://doi.org/10.1016/j.breast.2015.04.001

Breast cancer is the second most common malignancy diagnosed in women worldwide. The greatest cause of breast cancer mortality is development of metastasis. For many women metastasis is an early event in breast cancer which goes undetected until its... Read More about Circulating microRNAs for the prediction of metastasis in breast cancer patients diagnosed with early stage disease.

Autophagy receptor defects and ALS-FTLD (2015)
Journal Article
Majchera, V., Goode, A., James, V., & Layfield, R. (2015). Autophagy receptor defects and ALS-FTLD. Molecular and Cellular Neuroscience, 66(A), 43-52. doi:10.1016/j.mcn.2015.01.002

Various pathophysiological mechanisms have been implicated in the ALS-FTLD clinicopathological spectrum of neurodegenerative disorders. Here we focus on the role of autophagy, an intracellular catabolic pathway, in these conditions. Growing evidence... Read More about Autophagy receptor defects and ALS-FTLD.

SMG-1 and mTORC1 act antagonistically to regulate response to injury and growth in planarians (2012)
Journal Article
Gonzalez-Estevez, C., Felix, D. A., Smith, M. D., Paps, J., Morley, S. J., James, V., …Aboobaker, A. A. (2012). SMG-1 and mTORC1 act antagonistically to regulate response to injury and growth in planarians. PLoS Genetics, 8(3), Article e1002619. https://doi.org/10.1371/journal.pgen.1002619

Planarian flatworms are able to both regenerate their whole bodies and continuously adapt their size to nutrient status. Tight control of stem cell proliferation and differentiation during these processes is the key feature of planarian biology. Here... Read More about SMG-1 and mTORC1 act antagonistically to regulate response to injury and growth in planarians.

The LIMD1 protein bridges an association between the prolyl hydroxylases and VHL to repress HIF-1 activity (2012)
Journal Article
Foxler, D. E., Bridge, K. S., James, V., Webb, T. M., Mee, M., Wong, S. C. K., …Sharp, T. V. (2012). The LIMD1 protein bridges an association between the prolyl hydroxylases and VHL to repress HIF-1 activity. Nature Cell Biology, 14(2), 201-208. https://doi.org/10.1038/ncb2424

There are three prolyl hydroxylases (PHD1, 2 and 3) that regulate the hypoxia-inducible factors (HIFs), the master transcriptional regulators that respond to changes in intracellular O2 tension1,2. In high O2 tension (normoxia) the PHDs hydroxylate t... Read More about The LIMD1 protein bridges an association between the prolyl hydroxylases and VHL to repress HIF-1 activity.

PU.1 is a major transcriptional activator of the tumour suppressor gene LIMD1 (2011)
Journal Article
Foxler, D. E., James, V., Shelton, S. J., de A. Vallim, T. Q., Shaw, P. E., & Sharp, T. V. (2011). PU.1 is a major transcriptional activator of the tumour suppressor gene LIMD1. FEBS Letters, 585(7), 1089-1096. https://doi.org/10.1016/j.febslet.2011.03.013

LIMD1 is a tumour suppressor gene (TSG) down regulated in ?80% of lung cancers with loss also demonstrated in breast and head and neck squamous cell carcinomas. LIMD1 is also a candidate TSG in childhood acute lymphoblastic leukaemia. Mechanistically... Read More about PU.1 is a major transcriptional activator of the tumour suppressor gene LIMD1.

Maximising graduate status in pre-registration nursing programmes: Utilising problem based learning (2010)
Journal Article
McGarry, J., Aubeeluck, A., James, V., & Hinsliff-Smith, K. (2011). Maximising graduate status in pre-registration nursing programmes: Utilising problem based learning. Nurse Education in Practice, 11(6), 342-344. doi:10.1016/j.nepr.2010.11.018

This paper debates the use of problem based learning in accelerated pre-registration nursing programmes that are specifically designed for candidates with ‘graduate status’. We discuss the benefits of using problem based learning (PBL) within a gradu... Read More about Maximising graduate status in pre-registration nursing programmes: Utilising problem based learning.

Knockdown of microRNA-21 Inhibits Proliferation and Increases Cell Death by Targeting Programmed Cell Death 4 (PDCD4) in Pancreatic Ductal Adenocarcinoma (2010)
Journal Article
Bhatti, I., Lee, A., James, V., Hall, R. I., Lund, J. N., Tufarelli, C., …Larvin, M. (2011). Knockdown of microRNA-21 Inhibits Proliferation and Increases Cell Death by Targeting Programmed Cell Death 4 (PDCD4) in Pancreatic Ductal Adenocarcinoma. Journal of Gastrointestinal Surgery, 15(1), 199-208. https://doi.org/10.1007/s11605-010-1381-x

LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing (2010)
Journal Article
James, V., Zhang, Y., Foxler, D. E., de Moor, C. H., Kong, Y. W., Webb, T. M., …Sharp, T. V. (2010). LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing. Proceedings of the National Academy of Sciences, 107(28), 12499-12504. https://doi.org/10.1073/pnas.0914987107

In recent years there have been major advances with respect to the identification of the protein components and mechanisms of microRNA (miRNA) mediated silencing. However, the complete and precise repertoire of components and mechanism(s) of action r... Read More about LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing.

Mutations of the TGF-? type II receptor BMPR2 in pulmonary arterial hypertension (2006)
Journal Article
Machado, R. D., Aldred, M. A., James, V., Harrison, R. E., Patel, B., Schwalbe, E. C., …Trembath, R. C. (2006). Mutations of the TGF-β type II receptor BMPR2 in pulmonary arterial hypertension. Human Mutation, 27(2), 121-132. doi:10.1002/humu.20285

Pulmonary arterial hypertension (PAH) is clinically characterized by a sustained elevation in mean pulmonary artery pressure leading to significant morbidity and mortality. The disorder is typically sporadic, and in such cases the term idiopathic PAH... Read More about Mutations of the TGF-? type II receptor BMPR2 in pulmonary arterial hypertension.