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Diagnostic accuracy of autofluorescence-Raman microspectroscopy for surgical margin assessment during Mohs micrographic surgery of basal cell carcinoma (2024)
Journal Article
Boitor, R. A., Varma, S., Sharma, A., Odedra, S., Elsheikh, S., Eldib, K., …Notingher, I. (2024). Diagnostic accuracy of autofluorescence-Raman microspectroscopy for surgical margin assessment during Mohs micrographic surgery of basal cell carcinoma. British Journal of Dermatology, Article ljae196. https://doi.org/10.1093/bjd/ljae196

Background Autofluorescence (AF)–Raman microspectroscopy is a technology that can detect residual basal cell carcinoma (BCC) on the resection margin of fresh, surgically excised tissue specimens. The technology does not require tissue fixation, st... Read More about Diagnostic accuracy of autofluorescence-Raman microspectroscopy for surgical margin assessment during Mohs micrographic surgery of basal cell carcinoma.

Ex vivo assessment of basal cell carcinoma surgical margins in Mohs surgery by autofluorescence-Raman spectroscopy: A pilot study (2023)
Journal Article
Boitor, R., Varma, S., Sharma, A., Elsheikh, S., Kulkarni, K., Eldib, K., …Notingher, I. (2024). Ex vivo assessment of basal cell carcinoma surgical margins in Mohs surgery by autofluorescence-Raman spectroscopy: A pilot study. JEADV Clinical Practice, 3(2), 498-507. https://doi.org/10.1002/jvc2.336

Background: Autofluorescence (AF)‐Raman spectroscopy is a technology that can detect tumour tissue in surgically excised skin specimens. The technique does not require tissue fixation, staining, labelling or sectioning, and provides quantitative diag... Read More about Ex vivo assessment of basal cell carcinoma surgical margins in Mohs surgery by autofluorescence-Raman spectroscopy: A pilot study.

Optimization of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in a small animal model (2023)
Journal Article
Dooley, M., Luckett, J., Alexander, M. R., Matousek, P., Dehghani, H., Ghaemmaghami, A. M., & Notingher, I. (2023). Optimization of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in a small animal model. Biomedical Optics Express, 14(12), 6592-6606. https://doi.org/10.1364/boe.512118

Diffuse Raman spectroscopy (DRS) allows subsurface molecular analysis of optically turbid samples. Numerical modeling of light propagation was used as a method for improving the design of an DRS instrument to maximize the signal to noise ratio (SNR)... Read More about Optimization of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in a small animal model.

Optimisation of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in small animal model (2023)
Journal Article
Dooley, M., Luckett, J., Alexander, M. R., Matousek, P., Dehghani, H., Ghaemmaghami, A. M., & Notingher, I. (2023). Optimisation of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in small animal model. Biomedical Optics Express, 14(12), 6592-6606. https://doi.org/10.1364/BOE.512118

Diffuse Raman spectroscopy (DRS) allows subsurface molecular analysis of optically turbid samples. Numerical modelling of light propagation was used as a method for improving the design of an DRS instrument to maximize the signal to noise ratio (SNR)... Read More about Optimisation of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in small animal model.

Real-time ex-vivo assessment of basal cell carcinoma surgical margins in Mohs surgery by autofluorescence-Raman spectroscopy: a pilot study (2023)
Journal Article
Boitor, R., Varma, V., Sharma, A., Elsheikh, S., Kulkarni, K., Eldib, K., …Notingher, I. (in press). Real-time ex-vivo assessment of basal cell carcinoma surgical margins in Mohs surgery by autofluorescence-Raman spectroscopy: a pilot study. Journal of the European Academy of Dermatology and Venereology,

Background Autofluorescence (AF)-Raman spectroscopy is a technology that can detect tumour tissue in surgically excised skin specimens. The technique does not require tissue fixation, staining, labelling or sectioning, and provides quantitative diag... Read More about Real-time ex-vivo assessment of basal cell carcinoma surgical margins in Mohs surgery by autofluorescence-Raman spectroscopy: a pilot study.

Autofluorescence-Raman Spectroscopy for Ex Vivo Mapping Colorectal Liver Metastases and Liver Tissue (2023)
Journal Article
Corden, C., Boitor, R., Dusanjh, P. K., Harwood, A., Mukherjee, A., Gomez, D., & Notingher, I. (2023). Autofluorescence-Raman Spectroscopy for Ex Vivo Mapping Colorectal Liver Metastases and Liver Tissue. Journal of Surgical Research, 288, 10-20. https://doi.org/10.1016/j.jss.2023.02.014

Introduction: Identifying colorectal liver metastases (CRLM) during liver resection could assist in achieving clear surgical margins, which is an important prognostic variable for both disease-free and overall survival. The aim of this study was to i... Read More about Autofluorescence-Raman Spectroscopy for Ex Vivo Mapping Colorectal Liver Metastases and Liver Tissue.

Quantification of Drugs in Brain and Liver Mimetic Tissue Models Using Raman Spectroscopy (2022)
Journal Article
Woodhouse, N., Majer, J., Marshall, P., Hood, S., & Notingher, I. (2022). Quantification of Drugs in Brain and Liver Mimetic Tissue Models Using Raman Spectroscopy. Applied Spectroscopy, https://doi.org/10.1177/00037028221139494

Quantitative analysis of drug delivery with in biological systems is an integral challenge in drug development. Analytical techniques are important for assessing both drug target delivery, target action, and drug toxicology. Using mimetic tissue mode... Read More about Quantification of Drugs in Brain and Liver Mimetic Tissue Models Using Raman Spectroscopy.

Model-Based Optimization of Laser Excitation and Detection Improves Spectral Contrast in Noninvasive Diffuse Raman Spectroscopy (2022)
Journal Article
Dooley, M. J., Paterson, T., Dexter, L., Matousek, P., Dehghani, H., & Notingher, I. (2022). Model-Based Optimization of Laser Excitation and Detection Improves Spectral Contrast in Noninvasive Diffuse Raman Spectroscopy. Applied Spectroscopy, 76(7), 801-811. https://doi.org/10.1177/00037028211072900

Spatially offset Raman spectroscopy (SORS) is a powerful technique for subsurface molecular analysis of optically turbid samples. Numerical modeling of light propagation has been used to investigate opportunities for improving spectral contrast and s... Read More about Model-Based Optimization of Laser Excitation and Detection Improves Spectral Contrast in Noninvasive Diffuse Raman Spectroscopy.

Time-gated Raman spectroscopy for biomedical application under ambient or strong background light conditions (2021)
Journal Article
Corden, C., Boitor, R., & Notingher, I. (2021). Time-gated Raman spectroscopy for biomedical application under ambient or strong background light conditions. Journal of Physics D: Applied Physics, 54(50), Article 504003. https://doi.org/10.1088/1361-6463/ac276e

Many biomedical applications require measurements of Raman spectra of tissue under ambient lighting conditions. However, the background light often swamps the weaker Raman signal. The use of time-gated (TG) Raman spectroscopy based on a single photon... Read More about Time-gated Raman spectroscopy for biomedical application under ambient or strong background light conditions.

Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma (2021)
Journal Article
Boitor, R., de Wolf, C., Weesie, F., Shipp, D. W., Varma, S., Veitch, D., …Notingher, I. (2021). Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma. Biomedical Optics Express, 12(4), 2015-2026. https://doi.org/10.1364/boe.417896

We present the first clinical integration of a prototype device based on integrated auto-fluorescence imaging and Raman spectroscopy (Fast Raman device) for intra-operative assessment of surgical margins during Mohs micrographic surgery of basal cell... Read More about Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma.

Combined total internal reflection AF spectral-imaging and Raman spectroscopy for fast assessment of surgical margins during breast cancer surgery (2021)
Journal Article
Lizio, M. G., Liao, Z., Shipp, D. W., Boitor, R., Mihai, R., Sharp, J. S., …Notingher, I. (2021). Combined total internal reflection AF spectral-imaging and Raman spectroscopy for fast assessment of surgical margins during breast cancer surgery. Biomedical Optics Express, 12(2), 940-954. https://doi.org/10.1364/boe.411648

The standard treatment for breast cancer is surgical removal mainly through breast conserving surgey (BCS). We developed a new technique based on auto-fluorescence (AF) spectral imaging and Raman spectroscopy for fast intraoperative assessment of exc... Read More about Combined total internal reflection AF spectral-imaging and Raman spectroscopy for fast assessment of surgical margins during breast cancer surgery.

Sub-Surface Molecular Analysis and Imaging in Turbid Media Using Time-Gated Raman Spectral Multiplexing (2020)
Journal Article
Corden, C., Matousek, P., Conti, C., & Notingher, I. (2021). Sub-Surface Molecular Analysis and Imaging in Turbid Media Using Time-Gated Raman Spectral Multiplexing. Applied Spectroscopy, 75(2), 156-167. https://doi.org/10.1177/0003702820946054

Obtaining molecular information deeper within optically turbid samples is valuable in many applications. However, in many cases this is challenging, in particular when the sample elicits strong laser-induced fluorescence emission. Here, we investigat... Read More about Sub-Surface Molecular Analysis and Imaging in Turbid Media Using Time-Gated Raman Spectral Multiplexing.

Feasibility of integrated high‐wavenumber Raman imaging and fingerprint Raman spectroscopy for fast margin assessment in breast cancer surgery (2020)
Journal Article
Liao, Z., Lizio, M. G., Corden, C., Khout, H., Rakha, E., & Notingher, I. (2020). Feasibility of integrated high‐wavenumber Raman imaging and fingerprint Raman spectroscopy for fast margin assessment in breast cancer surgery. Journal of Raman Spectroscopy, 51(10), 1986-1995. https://doi.org/10.1002/jrs.5937

Intraoperative assessment of surgical margins remains one of the main challenges in cancer surgery. Raman spectroscopy can detect cancer cells with high accuracy, but it is time‐consuming. In this paper, we investigated a selective‐sampling Raman spe... Read More about Feasibility of integrated high‐wavenumber Raman imaging and fingerprint Raman spectroscopy for fast margin assessment in breast cancer surgery.

Induction and measurement of the early stage of a host-parasite interaction using a combined optical trapping and Raman microspectroscopy system (2019)
Journal Article
Sinjab, F., Elsheikha, H. M., Dooley, M., & Notingher, I. (2020). Induction and measurement of the early stage of a host-parasite interaction using a combined optical trapping and Raman microspectroscopy system. Journal of Biophotonics, 13(2), Article e201960065. https://doi.org/10.1002/jbio.201960065

© 2019 The Authors. Journal of Biophotonics published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Understanding and quantifying the temporal acquisition of host cell molecules by intracellular pathogens is fundamentally important in biology. In t... Read More about Induction and measurement of the early stage of a host-parasite interaction using a combined optical trapping and Raman microspectroscopy system.

Ex-vivo Raman spectroscopy mapping of lung tissue: label-free molecular characterisation of non-tumorous and cancerous tissues (2019)
Journal Article
Bourbousson, M., Soomro, I., Baldwin, D., & Notingher, I. (2019). Ex-vivo Raman spectroscopy mapping of lung tissue: label-free molecular characterisation of non-tumorous and cancerous tissues. Journal of Medical Imaging, 6(3), Article 036001. https://doi.org/10.1117/1.JMI.6.3.036001

Raman spectroscopy mapping was used to study ex vivo fresh lung tissues and compare to histology sections. The Raman mapping measurements revealed differences in the molecular composition of normal lung tissue, adenocarcinoma, and squamous cell carci... Read More about Ex-vivo Raman spectroscopy mapping of lung tissue: label-free molecular characterisation of non-tumorous and cancerous tissues.

Rapid production of human liver scaffolds for functional tissue engineering by high shear stress oscillation-decellularization (2017)
Journal Article
Mazza, G., Al-Akkad, W., Telese, A., Longato, L., Urbani, L., Robinson, B., …Pinzani, M. (in press). Rapid production of human liver scaffolds for functional tissue engineering by high shear stress oscillation-decellularization. Scientific Reports, 7, Article 5534. https://doi.org/10.1038/s41598-017-05134-1

The development of human liver scaffolds retaining their 3-dimensional structure and extra-cellular matrix (ECM) composition is essential for the advancement of liver tissue engineering. We report the design and validation of a new methodology for th... Read More about Rapid production of human liver scaffolds for functional tissue engineering by high shear stress oscillation-decellularization.

In-situ fabrication of gold nanoparticle functionalized probes for tip-enhanced Raman spectroscopy by dielectrophoresis (2016)
Journal Article
Sweetenham, C. S., Woolley, R., & Notingher, I. (2016). In-situ fabrication of gold nanoparticle functionalized probes for tip-enhanced Raman spectroscopy by dielectrophoresis. Journal of Nanophotonics, 10(3), 1-7. https://doi.org/10.1117/1.JNP.10.030502

We report the use of dielectrophoresis to fabricate in-situ probes for tip-enhanced Raman spectroscopy (TERS) based on Au nanoparticles. A typical conductive atomic force microscope (AFM) was used to functionalize iridium-coated conductive silicon pr... Read More about In-situ fabrication of gold nanoparticle functionalized probes for tip-enhanced Raman spectroscopy by dielectrophoresis.

Tracking amino acid’s uptake into the protozoan Acanthamoeba castellanii by stable-isotope labelling and Raman spectral imaging (2016)
Presentation / Conference Contribution
Naemat, A., Elsheikha, H. M., & Notingher, I. (2016). Tracking amino acid’s uptake into the protozoan Acanthamoeba castellanii by stable-isotope labelling and Raman spectral imaging. Proceedings of SPIE, 9887, Article 988715. https://doi.org/10.1117/12.2227070

The capacity of pathogens to acquire nutrients from their host cells is one of the most fundamental aspects of infection biology. Hence, measuring the patterns of nutrients’ uptake by pathogens is essential for understanding the interactions of patho... Read More about Tracking amino acid’s uptake into the protozoan Acanthamoeba castellanii by stable-isotope labelling and Raman spectral imaging.

Surface-enhanced Raman spectroscopy study of 4-ATP on gold nanoparticles for basal cell carcinoma fingerprint detection (2016)
Journal Article
Quynh, L. M., Nam, N. H., Kong, K., Nhung, N. T., Notingher, I., Henini, M., & Luong, N. H. (2016). Surface-enhanced Raman spectroscopy study of 4-ATP on gold nanoparticles for basal cell carcinoma fingerprint detection. Journal of Electronic Materials, 45(5), 2563-2568. https://doi.org/10.1007/s11664-016-4421-9

The surface-enhanced Raman signals of 4-aminothiophenol (4-ATP) attached to the surface of colloidal gold nanoparticles with size distribution of 2 to 5 nm were used as a labeling agent to detect basal cell carcinoma (BCC) of the skin. The enhanced R... Read More about Surface-enhanced Raman spectroscopy study of 4-ATP on gold nanoparticles for basal cell carcinoma fingerprint detection.

Tracing amino acid exchange during host-pathogen interaction by combined stable-isotope time-resolved Raman spectral imaging (2016)
Journal Article
Naemat, A., Elsheikha, H. M., Boiter, R., & Notingher, I. (2016). Tracing amino acid exchange during host-pathogen interaction by combined stable-isotope time-resolved Raman spectral imaging. Scientific Reports, 6, Article 20811. https://doi.org/10.1038/srep20811

This study investigates the temporal and spatial interchange of the aromatic amino acid phenylalanine (Phe) between human retinal pigment epithelial cell line (ARPE-19) and tachyzoites of the apicomplexan protozoan parasite Toxoplasma gondii (T. gond... Read More about Tracing amino acid exchange during host-pathogen interaction by combined stable-isotope time-resolved Raman spectral imaging.

Diagnosis of tumors during tissue-conserving surgery with integrated autofluorescence and Raman scattering microscopy (2013)
Journal Article
Kong, K., Rowlands, C. J., Varma, S., Perkins, W., Leach, I. H., Koloydenko, A. A., …Notingher, I. (2013). Diagnosis of tumors during tissue-conserving surgery with integrated autofluorescence and Raman scattering microscopy. Proceedings of the National Academy of Sciences, 110(38), https://doi.org/10.1073/pnas.1311289110

Tissue-conserving surgery is used increasingly in cancer treatment. However, one of the main challenges in this type of surgery is the detection of tumor margins. Histopathology based on tissue sectioning and staining has been the gold standard for c... Read More about Diagnosis of tumors during tissue-conserving surgery with integrated autofluorescence and Raman scattering microscopy.

Fingering Instabilities in Dewetting Nanofluids (2008)
Journal Article
Pauliac-Vaujour, E., Stannard, A., Martin, C., Blunt, M., Notingher, I., Moriarty, P., …Thiele, U. (2008). Fingering Instabilities in Dewetting Nanofluids. Physical Review Letters, 100,

The growth of fingering patterns in dewetting nanofluids (colloidal solutions of thiol-passivated gold nanoparticles) has been followed in real time using contrast-enhanced video microscopy. The fingering instability on which we focus here arises fro... Read More about Fingering Instabilities in Dewetting Nanofluids.