Combined total internal reflection AF spectral-imaging and Raman spectroscopy for fast assessment of surgical margins during breast cancer surgery

Lizio, Maria Giovanna; Liao, Zhiyu; Shipp, Dustin W.; Boitor, Radu; Mihai, Raluca; Sharp, James S.; Russell, Matthew; Khout, Hazem; Rakha, Emad A.; Notingher, Ioan

Abstract

The standard treatment for breast cancer is surgical removal mainly through breast conserving surgey (BCS). We developed a new technique based on auto-fluorescence (AF) spectral imaging and Raman spectroscopy for fast intraoperative assessment of excision margins in BCS. A new wide-field AF imaging unit based on total internal reflection (TIR) was combined with a Raman spectroscopy microscope equipped with a 785 nm laser. The wavelength of the AF excitation was optimized to 365 nm in order to maximize the discrimination of adipose tissue. This approach allows for the non-adipose regions of tissue, which are at higher-risk of containing tumor, to be targeted more efficiently by the Raman spectroscopy measurements. The integrated TIR-AF-Raman was tested on small tissue samples as well as fresh wide local excisions, delivering the analysis of the entire cruciate surface of BCS specimens (5.1 × 7.6 cm 2) in less than 45 minutes and also providing information regarding the location of the tumour in the specimen. Full automation of the instrument and selection of a faster translation stage would allow for the measurement of BCS specimens within intraoperative time scale (20 minutes). This study demonstrates that the TIR-AF Raman microscope represents a feasible step towards the development of a technique for intraoperative assessment of large WLE within intraoperative timescales.

Citation

Lizio, M. G., Liao, Z., Shipp, D. W., Boitor, R., Mihai, R., Sharp, J. S., …Notingher, I. (2021). Combined total internal reflection AF spectral-imaging and Raman spectroscopy for fast assessment of surgical margins during breast cancer surgery. Biomedical Optics Express, 12(2), 940-954. https://doi.org/10.1364/boe.411648

• Journal Article Type: Article
• Acceptance Date: Nov 30, 2020
• Online Publication Date: Jan 19, 2021
• Publication Date: Feb 1, 2021
• Deposit Date: Dec 8, 2020
• Publicly Available Date: Jan 19, 2021
• Journal: Biomedical Optics Express
• Electronic ISSN: 2156-7085
• Publisher: Optical Society of America
• Peer Reviewed: Peer Reviewed
• Volume: 12
• Issue: 2
• Pages: 940-954
• DOI: https://doi.org/10.1364/boe.411648
• Keywords: Biotechnology; Atomic and Molecular Physics, and Optics
• Public URL: https://nottingham-repository.worktribe.com/output/5128097
• Publisher URL: https://www.osapublishing.org/boe/fulltext.cfm?uri=boe-12-2-940&id=446681
• Additional Information: This article is maintained by: OSA - The Optical Society; Crossref DOI link to publisher maintained version: https://doi.org/10.1364/BOE.411648; Article type: research-article; Similarity check: Screened by Similarity Check; Peer reviewed: Yes; Review process: Single blind; Received: 5 October 2020; Accepted: 29 November 2020; Published: 19 January 2021; Copyright: Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.