All Outputs (183)

Valorisation of hop leaves for their bioactive compounds: Identification and quantification of phenolics across different varieties, crop years and stages of development (2024)
Journal Article
Calvert, D., Dew, T., Gadon, A., Gros, J., & Cook, D. (2025). Valorisation of hop leaves for their bioactive compounds: Identification and quantification of phenolics across different varieties, crop years and stages of development. Food Chemistry, 465(Part 2), Article 142005. https://doi.org/10.1016/j.foodchem.2024.142005

Hop leaves, a by-product from hop cone harvesting, contain phenolic compounds of potential value for food or beverage applications. However, the abundant phenolics in hop leaves remain largely unquantified. This study quantified phenolics in hop leav... Read More about Valorisation of hop leaves for their bioactive compounds: Identification and quantification of phenolics across different varieties, crop years and stages of development.

Multiplexing projects: supervising undergraduate research projects with larger cohorts (2024)
Journal Article
Nisbet, R. E. R., Scott, D. J., & Campeotto, I. (2024). Multiplexing projects: supervising undergraduate research projects with larger cohorts. The Biochemist, 46(5), 18-23. https://doi.org/10.1042/BIO_2024_897

Almost all undergraduate students in the UK complete a final year research project or dissertation. In the molecular biosciences field, these projects are often based in individual research laboratory. However, with increasing student numbers, fallin... Read More about Multiplexing projects: supervising undergraduate research projects with larger cohorts.

Impacts of starch breakdown during germination and stewing on aroma formation in crystal malts (2024)
Journal Article
Foulkes, A. R., Bolat, I., Dodds, C., & Cook, D. J. (2024). Impacts of starch breakdown during germination and stewing on aroma formation in crystal malts. Journal of the Institute of Brewing, 130(4), 221-237. https://doi.org/10.58430/jib.v130i4.60

Why was the work done: Crystal malts are used in brewing to add colour, flavour and mouthfeel to beers. In their manufacture, aroma compounds are formed through thermal flavour generation during roasting of stewed green malt. As part of a wider proje... Read More about Impacts of starch breakdown during germination and stewing on aroma formation in crystal malts.

Extreme enthalpy‒entropy compensation in the dimerization of small solutes in aqueous solution (2024)
Journal Article
Scott, D. J., & Winzor, D. J. (2024). Extreme enthalpy‒entropy compensation in the dimerization of small solutes in aqueous solution. European Biophysics Journal, 53, 373-384. https://doi.org/10.1007/s00249-024-01722-y

This communication summarizes findings from the earliest encounters with extreme enthalpy‒entropy compensation, a phenomenon first detected in the 1950s by a reappraisal of isopiestic and calorimetric measurements on aqueous urea solutions in terms o... Read More about Extreme enthalpy‒entropy compensation in the dimerization of small solutes in aqueous solution.

Is there reversible dimerization of albumin in blood plasma? And does it matter? (2024)
Journal Article
Harris, G., Bradshaw, M. L., Halsall, D. J., Scott, D. J., Unwin, R. J., & Norden, A. G. W. (2024). Is there reversible dimerization of albumin in blood plasma? And does it matter?. Experimental Physiology, 109(10), 1663-1671. https://doi.org/10.1113/EP092012

Most albumin in blood plasma is thought to be monomeric with some 5% covalently dimerized. However, many reports in the recent biophysics literature find that albumin is reversibly dimerized or even oligomerized. We review data on this from X‐ray cry... Read More about Is there reversible dimerization of albumin in blood plasma? And does it matter?.

Prebiotic galactooligosaccharide feed modifies the chicken gut microbiota to efficiently clear Salmonella (2024)
Journal Article
Richards, P. J., Almutrafy, A., Liang, L., Flaujac Lafontaine, G. M., King, E., Fish, N. M., Connerton, A. J., Connerton, P. L., & Connerton, I. F. (2024). Prebiotic galactooligosaccharide feed modifies the chicken gut microbiota to efficiently clear Salmonella. mSystems, 9(8), Article e00754-24. https://doi.org/10.1128/msystems.00754-24

Chicken meat is contaminated with Salmonella from the gut of infected chickens during slaughter. Eradication of Salmonella from broiler chickens through hygiene measures and/or vaccination is not cost-effective; complementary approaches are required.... Read More about Prebiotic galactooligosaccharide feed modifies the chicken gut microbiota to efficiently clear Salmonella.

Influence of ripening stage on the microwave-assisted pectin extraction from banana peels: A feasibility study targeting both the Homogalacturonan and Rhamnogalacturonan-I region. (2024)
Journal Article
Mao, Y., Dewi, S. R., Harding, S. E., & Binner, E. (2024). Influence of ripening stage on the microwave-assisted pectin extraction from banana peels: A feasibility study targeting both the Homogalacturonan and Rhamnogalacturonan-I region. Food Chemistry, 460(Pt 1), Article 140549. https://doi.org/10.1016/j.foodchem.2024.140549

This work investigated a sustainable and efficient approach of pectin extraction for banana peel waste valorisation and studied the influence of banana ripening stages (RS at 2,5 and 7). Although pectin content in banana peel raw material decreased d... Read More about Influence of ripening stage on the microwave-assisted pectin extraction from banana peels: A feasibility study targeting both the Homogalacturonan and Rhamnogalacturonan-I region..

Pilot Evaluation of S-(3-[18F]Fluoropropyl)-d-Homocysteine and O-(2-[18F]Fluoroethyl)-d-Tyrosine as Bacteria-Specific Radiotracers for PET Imaging of Infection (2024)
Journal Article
Betts, H. M., Luckett, J. C., & Hill, P. J. (2024). Pilot Evaluation of S-(3-[18F]Fluoropropyl)-d-Homocysteine and O-(2-[18F]Fluoroethyl)-d-Tyrosine as Bacteria-Specific Radiotracers for PET Imaging of Infection. Molecular Imaging and Biology, 26, 704-713. https://doi.org/10.1007/s11307-024-01929-7

Purpose There is currently no ideal radiotracer for imaging bacterial infections. Radiolabelled d-amino acids are promising candidates because they are actively incorporated into the peptidoglycan of the bacterial cell wall, a structural feature whic... Read More about Pilot Evaluation of S-(3-[18F]Fluoropropyl)-d-Homocysteine and O-(2-[18F]Fluoroethyl)-d-Tyrosine as Bacteria-Specific Radiotracers for PET Imaging of Infection.

Storability and Brewing Quality of Corn Grits: Impacts of Storage Temperature, Particle Size and Key Compositional Factors (2024)
Journal Article
Maia, C., & Cook, D. J. (2024). Storability and Brewing Quality of Corn Grits: Impacts of Storage Temperature, Particle Size and Key Compositional Factors. Journal of The American Society of Brewing Chemists, https://doi.org/10.1080/03610470.2024.2338609

Across international brewing operations corn grits from different suppliers vary substantially in terms of their physical properties and chemical composition. Present research aimed to highlight the key factors determining the brewing quality of stor... Read More about Storability and Brewing Quality of Corn Grits: Impacts of Storage Temperature, Particle Size and Key Compositional Factors.

The Potential of Plant-Produced Virus-like Particle Vaccines for African Horse Sickness and Other Equine Orbiviruses (2024)
Journal Article
Pitchers, K. G., Boakye, O. D., Campeotto, I., & Daly, J. M. (2024). The Potential of Plant-Produced Virus-like Particle Vaccines for African Horse Sickness and Other Equine Orbiviruses. Pathogens, 13(6), Article 458. https://doi.org/10.3390/pathogens13060458

African horse sickness is a devastating viral disease of equids. It is transmitted by biting midges of the genus Culicoides with mortalities reaching over 90% in naïve horses. It is endemic to sub-Saharan Africa and is seasonally endemic in many part... Read More about The Potential of Plant-Produced Virus-like Particle Vaccines for African Horse Sickness and Other Equine Orbiviruses.

Integrating cryo-OrbiSIMS with computational modelling and metadynamics simulations enhances RNA structure prediction at atomic resolution (2024)
Journal Article
Ward, S., Childs, A., Staley, C., Waugh, C., Watts, J. A., Kotowska, A. M., Bhosale, R., & Borkar, A. N. (2024). Integrating cryo-OrbiSIMS with computational modelling and metadynamics simulations enhances RNA structure prediction at atomic resolution. Nature Communications, 15(1), Article 4367. https://doi.org/10.1038/s41467-024-48694-3

The 3D architecture of RNAs governs their molecular interactions, chemical reactions, and biological functions. However, a large number of RNAs and their protein complexes remain poorly understood due to the limitations of conventional structural bio... Read More about Integrating cryo-OrbiSIMS with computational modelling and metadynamics simulations enhances RNA structure prediction at atomic resolution.

Optimisation of Saccharomyces cerevisiae BRYC 501 ascospore formation and recovery for heat inactivation experiments (2024)
Journal Article
Raleigh, C. P., Lawrence, S., & Rachon, G. (2024). Optimisation of Saccharomyces cerevisiae BRYC 501 ascospore formation and recovery for heat inactivation experiments. Journal of the Institute of Brewing, 130(2), 70-82. https://doi.org/10.58430/jib.v130i2.48

Why was the work done: Ascospores from Saccharomyces cerevisiae BRYC 501 are useful biological indicators for validating the pasteurisation of beer. Ascospores are formed by yeast in response to low nutrient conditions and are characterised by greate... Read More about Optimisation of Saccharomyces cerevisiae BRYC 501 ascospore formation and recovery for heat inactivation experiments.

Open and closed conformations of a sub-80 kDa Chagas vaccine candidate defined by a cryo-EM led integrative approach (2024)
Preprint / Working Paper
Batra, S., Ragan, T. J., Frank, A. M., Kaplan, M., Lancey, C., Assadipapari, M., Ying, C., Struwe, W. B., Hesketh, E., Barfod, L., & Campeotto, I. Open and closed conformations of a sub-80 kDa Chagas vaccine candidate defined by a cryo-EM led integrative approach

Chagas disease, caused by the protozoan parasite Trypanosoma cruzi, remains a significant global public health concern. It affects an estimated eight million individuals worldwide, with the majority remaining undiagnosed. Despite its profound health... Read More about Open and closed conformations of a sub-80 kDa Chagas vaccine candidate defined by a cryo-EM led integrative approach.

Untargeted metabolomic profiling of 100% malt beers versus those containing barley adjunct (2024)
Journal Article
Yorke, J., Dew, T., & Cook, D. (2024). Untargeted metabolomic profiling of 100% malt beers versus those containing barley adjunct. Journal of the Institute of Brewing, 130(1), 31-46. https://doi.org/10.58430/jib.v130i1.46

Why was the work done: The incorporation of unmalted barley at high proportion in the grist can introduce unfavourable bitter and astringent characteristics to beer, resulting in an upper limit on the proportion used. The flavour active compounds fro... Read More about Untargeted metabolomic profiling of 100% malt beers versus those containing barley adjunct.

The need for One Health systems-thinking approaches to understand multiscale dissemination of antimicrobial resistance (2024)
Journal Article
Arnold, K. E., Laing, G., McMahon, B. J., Fanning, S., Stekel, D. J., Pahl, O., Coyne, L., Latham, S. M., & McIntyre, K. M. (2024). The need for One Health systems-thinking approaches to understand multiscale dissemination of antimicrobial resistance. The Lancet Planetary Health, 8(2), E124-E133. https://doi.org/10.1016/S2542-5196%2823%2900278-4

Although the effects of antimicrobial resistance (AMR) are most obvious at clinical treatment failure, AMR evolution, transmission, and dispersal happen largely in environmental settings, for example within farms, waterways, livestock, and wildlife.... Read More about The need for One Health systems-thinking approaches to understand multiscale dissemination of antimicrobial resistance.

The long and short of it: benchmarking viromics using Illumina, Nanopore and PacBio sequencing technologies (2024)
Journal Article
Cook, R., Brown, N., Rihtman, B., Michniewski, S., Redgwell, T., Clokie, M., Stekel, D. J., Chen, Y., Scanlan, D. J., Hobman, J. L., Nelson, A., Jones, M. A., Smith, D., & Millard, A. (2024). The long and short of it: benchmarking viromics using Illumina, Nanopore and PacBio sequencing technologies. Microbial Genomics, 10(2), Article 0.001198. https://doi.org/10.1099/mgen.0.001198

Viral metagenomics has fuelled a rapid change in our understanding of global viral diversity and ecology. Long-read sequencing and hybrid assembly approaches that combine long- and short-read technologies are now being widely implemented in bacterial... Read More about The long and short of it: benchmarking viromics using Illumina, Nanopore and PacBio sequencing technologies.

The two faces of microorganisms in traditional brewing and the implications for no- and low-alcohol beers (2024)
Journal Article
Roselli, G. E., Kerruish, D. W. M., Crow, M., Smart, K. A., & Powell, C. D. (2024). The two faces of microorganisms in traditional brewing and the implications for no- and low-alcohol beers. Frontiers in Microbiology, 15, Article 1346724. https://doi.org/10.3389/fmicb.2024.1346724

The production of alcoholic beverages is intrinsically linked to microbial activity. This is because microbes such as yeast are associated with the production of ethanol and key sensorial compounds that produce desirable qualities in fermented produc... Read More about The two faces of microorganisms in traditional brewing and the implications for no- and low-alcohol beers.

Development of an open-source carbon footprint calculator of the UK craft brewing value chain (2023)
Journal Article
Bowler, A. L., Rodgers, S., Meng, F., McKechnie, J., Cook, D. J., & Watson, N. J. (2024). Development of an open-source carbon footprint calculator of the UK craft brewing value chain. Journal of Cleaner Production, 435, Article 140181. https://doi.org/10.1016/j.jclepro.2023.140181

Craft breweries may fall behind large brewing companies in reducing the carbon footprints of their value chains due to limited resources, financial constraints, and a lack of technical knowledge to fully understand their emissions. However, by increa... Read More about Development of an open-source carbon footprint calculator of the UK craft brewing value chain.

Modeling of Space Crop-Based Dishes for Optimal Nutrient Delivery to Astronauts and Beyond on Earth (2023)
Journal Article
Liang, S., Rivera-Osorio, K., Burgess, A. J., Kumssa, D. B., Escribà-Gelonch, M., Fisk, I., Knowling, M., Stekel, D., & Hessel, V. (2024). Modeling of Space Crop-Based Dishes for Optimal Nutrient Delivery to Astronauts and Beyond on Earth. ACS Food Science & Technology, 4(1), 104–117. https://doi.org/10.1021/acsfoodscitech.3c00396

Future long-term human exploration of space will need a supply of resources for astronauts, including fresh food from space farms. This means it is necessary to identify combinations of crops that can be successfully grown together and which provide... Read More about Modeling of Space Crop-Based Dishes for Optimal Nutrient Delivery to Astronauts and Beyond on Earth.

Campylobacter Bacteriophage Infection at Refrigeration Temperatures (2023)
Journal Article
Hu, Y., Al Shaaer, B., Liang, L., & Connerton, I. F. (2023). Campylobacter Bacteriophage Infection at Refrigeration Temperatures. Applied Microbiology, 3(4), 1392-1406. https://doi.org/10.3390/applmicrobiol3040094

The application of bacteriophages to control foodborne bacterial pathogens in foods has gained traction in recent years. Poultry meat is a major source of Campylobacter jejuni, and a target for the application of bacteriophages. To offer the prospect... Read More about Campylobacter Bacteriophage Infection at Refrigeration Temperatures.