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Simultaneous measurement of excitation-contraction coupling parameters identifies mechanisms underlying contractile responses of hiPSC-derived cardiomyocytes (2019)
Journal Article
Van Meer, B. J., Krotenberg, A., Sala, L., Davis, R. P., Eschenhagen, T., Denning, C., …Mummery, C. L. (2019). Simultaneous measurement of excitation-contraction coupling parameters identifies mechanisms underlying contractile responses of hiPSC-derived cardiomyocytes. Nature Communications, 10, Article 4325. https://doi.org/10.1038/s41467-019-12354-8

Cardiomyocytes from human induced pluripotent stem cells (hiPSC-CMs) are increasingly recognized as valuable for determining the effects of drugs on ion channels but they do not always accurately predict contractile responses of the human heart. This... Read More about Simultaneous measurement of excitation-contraction coupling parameters identifies mechanisms underlying contractile responses of hiPSC-derived cardiomyocytes.

The intra-tumoral stroma in patients with breast cancer increases with age (2019)
Journal Article
Vangangelt, K. M. H., Kramer, C. J. H., Bastiaannet, E., Putter, H., Cohen, D., van Pelt, G. W., …Mesker, W. E. (2020). The intra-tumoral stroma in patients with breast cancer increases with age. Breast Cancer Research and Treatment, 179(1), 37–45. https://doi.org/10.1007/s10549-019-05422-6

Purpose
The tumor microenvironment in older patients is subject to changes. The tumor-stroma ratio (TSR) was evaluated in order to estimate the amount of intra-tumoral stroma and to evaluate the prognostic value of the TSR in older patients with br... Read More about The intra-tumoral stroma in patients with breast cancer increases with age.

Neuronal over-expression of Oxr1 is protective against ALS-associated mutant TDP-43 mislocalisation in motor neurons and neuromuscular defects in vivo (2019)
Journal Article
Williamson, M. G., Finelli, M. J., Sleigh, J. N., Reddington, A., Gordon, D., Talbot, K., …Oliver, P. L. (2019). Neuronal over-expression of Oxr1 is protective against ALS-associated mutant TDP-43 mislocalisation in motor neurons and neuromuscular defects in vivo. Human Molecular Genetics, 28(21), 3584-3599. https://doi.org/10.1093/hmg/ddz190

A common pathological hallmark of amyotrophic lateral sclerosis (ALS) and the related neurodegenerative disorder frontotemporal dementia, is the cellular mislocalization of transactive response DNA-binding protein 43 kDa (TDP-43). Additionally, multi... Read More about Neuronal over-expression of Oxr1 is protective against ALS-associated mutant TDP-43 mislocalisation in motor neurons and neuromuscular defects in vivo.

Validation and assessment of an antibiotic-based, aseptic decontamination manufacturing protocol for therapeutic, vacuum-dried human amniotic membrane (2019)
Journal Article
Marsit, N. M., Sidney, L. E., Britchford, E. R., McIntosh, O. D., Allen, C. L., Ashraf, W., …Hopkinson, A. (2019). Validation and assessment of an antibiotic-based, aseptic decontamination manufacturing protocol for therapeutic, vacuum-dried human amniotic membrane. Scientific Reports, 9(1), Article 12854. https://doi.org/10.1038/s41598-019-49314-7

© 2019, The Author(s). Amniotic membrane (AM) is used to treat a range of ophthalmic indications but must be presented in a non-contaminated state. AM from elective caesarean sections contains natural microbial contamination, requiring removal during... Read More about Validation and assessment of an antibiotic-based, aseptic decontamination manufacturing protocol for therapeutic, vacuum-dried human amniotic membrane.

Activated zinc transporter ZIP7 as an indicator of anti-hormone resistance in breast cancer (2019)
Journal Article
Taylor, K. M., Green, A. R., Ellis, I. O., Gee, J. M. W., Ziliotto, S., Zilio, S., …Taylor, K. M. (2019). Activated zinc transporter ZIP7 as an indicator of anti-hormone resistance in breast cancer. Metallomics, 11(9), 1579-1592. https://doi.org/10.1039/c9mt00136k

ZIP7, a member of the ZIP family of zinc importers, resides on the endoplasmic reticulum membrane and transports zinc from intracellular stores to the cytoplasm after activation by CK2 phosphorylation on two serine residues (S275 and S276). ZIP7 is k... Read More about Activated zinc transporter ZIP7 as an indicator of anti-hormone resistance in breast cancer.

CDC20 expression in oestrogen receptor positive breast cancer predicts poor prognosis and lack of response to endocrine therapy (2019)
Journal Article
Alfarsi, L., Ansari, R. E., Craze, M. L., Toss, M. S., Masisi, B., Ellis, I. O., …Green, A. R. (2019). CDC20 expression in oestrogen receptor positive breast cancer predicts poor prognosis and lack of response to endocrine therapy. Breast Cancer Research and Treatment, 178(3), 535-544. https://doi.org/10.1007/s10549-019-05420-8

Purpose
Endocrine therapy is the standard treatment for oestrogen receptor positive (ER+) breast cancer. Despite its efficacy, around half of patients will develop resistance to this treatment and eventually relapse. Identification of effective and... Read More about CDC20 expression in oestrogen receptor positive breast cancer predicts poor prognosis and lack of response to endocrine therapy.

Prognostic significance of cathepsin V (CTSV/CTSL2) in breast ductal carcinoma in situ (2019)
Journal Article
Toss, M. S., Miligy, I. M., Gorringe, K. L., Mittal, K., Green, A. R., Aneja, R., …Rakha, E. A. (2020). Prognostic significance of cathepsin V (CTSV/CTSL2) in breast ductal carcinoma in situ. Journal of Clinical Pathology, 73(2), 76-82. https://doi.org/10.1136/jclinpath-2019-205939

Aims: Cathepsin V (CTSV/CTSL2) is a lysosomal cystine proteinase and plays a role in extracellular matrix degradation. It is associated with poor prognosis in invasive breast cancer (IBC), but its role in breast ductal carcinoma in situ (DCIS) remain... Read More about Prognostic significance of cathepsin V (CTSV/CTSL2) in breast ductal carcinoma in situ.

Thioredoxin system protein expression in carcinomas of the pancreas, bile duct and ampulla (2019)
Preprint / Working Paper
Al-hadyan, K., Storr, S., Zaitoun, A., Lobo, D., & Martin, S. Thioredoxin system protein expression in carcinomas of the pancreas, bile duct and ampulla

Background: Pancreatic cancer (PC), including the ampulla and bile duct, is very aggressive, and thus difficult to treat with effective therapies. The current treatment options have failed to improve PC five-year survival rates over the last 30 to 40... Read More about Thioredoxin system protein expression in carcinomas of the pancreas, bile duct and ampulla.

ERCC1 Is a Predictor of Anthracycline Resistance and Taxane Sensitivity in Early Stage or Locally Advanced Breast Cancers (2019)
Journal Article
Abdel-Fatah, T. M., Ali, R., Sadiq, M., Moseley, P. M., Mesquita, K. A., Ball, G., …Madhusudan, S. (2019). ERCC1 Is a Predictor of Anthracycline Resistance and Taxane Sensitivity in Early Stage or Locally Advanced Breast Cancers. Cancers, 11(8), Article 1149. https://doi.org/10.3390/cancers11081149

Genomic instability could be a beneficial predictor for anthracycline or taxane chemotherapy. We interrogated 188 DNA repair genes in the METABRIC cohort (n = 1980) to identify genes that influence overall survival (OS). We then evaluated the clinico... Read More about ERCC1 Is a Predictor of Anthracycline Resistance and Taxane Sensitivity in Early Stage or Locally Advanced Breast Cancers.

Machine learning-based prediction of breast cancer growth rate in-vivo (2019)
Journal Article
Bhattarai, S., Klimov, S., Aleskandarany, M. A., Burrell, H., Wormall, A., Green, A. R., …Aneja, R. (2019). Machine learning-based prediction of breast cancer growth rate in-vivo. British Journal of Cancer, 121, 497–504. https://doi.org/10.1038/s41416-019-0539-x

Background
Determining the rate of breast cancer (BC) growth in vivo, which can predict prognosis, has remained elusive despite its relevance for treatment, screening recommendations and medicolegal practice. We developed a model that predicts the r... Read More about Machine learning-based prediction of breast cancer growth rate in-vivo.

Whole‐exome sequencing and RNA sequencing analyses of acinic cell carcinomas of the breast (2019)
Journal Article
Beca, F., Lee, S. S., Pareja, F., da Cruz Paula, A., Selenica, P., Ferrando, L., …Reis‐Filho, J. S. (2019). Whole‐exome sequencing and RNA sequencing analyses of acinic cell carcinomas of the breast. Histopathology, 75(6), 931-937. https://doi.org/10.1111/his.13962

Aims
Acinic cell carcinoma of the breast (ACC) is a rare histologic form of triple‐negative breast cancer (TNBC). Despite its unique histology, targeted sequencing analysis has failed to identify recurrent genetic alterations other than those found... Read More about Whole‐exome sequencing and RNA sequencing analyses of acinic cell carcinomas of the breast.

A whole slide image-based machine learning approach to predict ductal carcinoma in situ (DCIS) recurrence risk (2019)
Journal Article
Klimov, S., Miligy, I. M., Gertych, A., Jiang, Y., Toss, M. S., Rida, P., …Aneja, R. (2019). A whole slide image-based machine learning approach to predict ductal carcinoma in situ (DCIS) recurrence risk. Breast Cancer Research, 21(1), Article 83. https://doi.org/10.1186/s13058-019-1165-5

© 2019 The Author(s). Background: Breast ductal carcinoma in situ (DCIS) represent approximately 20% of screen-detected breast cancers. The overall risk for DCIS patients treated with breast-conserving surgery stems almost exclusively from local recu... Read More about A whole slide image-based machine learning approach to predict ductal carcinoma in situ (DCIS) recurrence risk.

Surgical management of ductal carcinoma in situ of the breast: A large retrospective study from a single institution (2019)
Journal Article
Miligy, I. M., Toss, M. S., Khout, H., Hc, B., Ellis, I. O., Green, A. R., …Rakha, E. (2019). Surgical management of ductal carcinoma in situ of the breast: A large retrospective study from a single institution. Breast Journal, 25(6), 1143-1153. https://doi.org/10.1111/tbj.13425

Background: Management of breast ductal carcinoma in situ (DCIS) has various approaches with distinct institutional specific practice. Here, we review DCIS management in a single institution with emphasise on re-operation rates and outcome. Methods:... Read More about Surgical management of ductal carcinoma in situ of the breast: A large retrospective study from a single institution.

Modeling Hypertrophic Cardiomyopathy: Mechanistic Insights and Pharmacological Intervention (2019)
Journal Article
Bhagwan, J. R., Smith, J. G., Mosqueira, D., Smith, J. G. W., Bhagwan, J. R., & Denning, C. (2019). Modeling Hypertrophic Cardiomyopathy: Mechanistic Insights and Pharmacological Intervention. Trends in Molecular Medicine, 25(9), 775-790. https://doi.org/10.1016/j.molmed.2019.06.005

HCM is a prevalent and complex disease governed by multiple molecular mechanisms, and there is currently no efficient cure. New treatment strategies are under development, and several drugs are reaching the final stages of clinical trials, with parti... Read More about Modeling Hypertrophic Cardiomyopathy: Mechanistic Insights and Pharmacological Intervention.

Live Simultaneous Monitoring of Mineral Deposition and Lipid Accumulation in Differentiating Stem Cells (2019)
Journal Article
De Melo, N., McGinlay, S., Markus, R., Macri-Pellizzeri, L., Symonds, M. E., Ahmed, I., & Sottile, V. (2019). Live Simultaneous Monitoring of Mineral Deposition and Lipid Accumulation in Differentiating Stem Cells. Biomimetics, 4(3), 1-10. https://doi.org/10.3390/biomimetics4030048

Mesenchymal stem cells (MSCs) are progenitors for bone-forming osteoblasts and lipid-storing adipocytes, two major lineages co-existing in bone marrow. When isolated in vitro, these stem cells recapitulate osteoblast or adipocyte formation if treated... Read More about Live Simultaneous Monitoring of Mineral Deposition and Lipid Accumulation in Differentiating Stem Cells.

Cten promotes Epithelial–Mesenchymal Transition (EMT) in colorectal cancer through stabilisation of Src (2019)
Journal Article
Asiri, A., Toss, M. S., Raposo, T. P., Akhlaq, M., Thorpe, H., Alfahed, A., …Ilyas, M. (2019). Cten promotes Epithelial–Mesenchymal Transition (EMT) in colorectal cancer through stabilisation of Src. Pathology International, 69(7), 381-391. https://doi.org/10.1111/pin.12811

Cten is an oncogene promoting EMT in many signaling pathways, namely through Snail. We investigated whether Cten function could be mediated through Src. Cten levels were modulated by forced expression in HCT116 and gene knockdown in SW620 CRC (colore... Read More about Cten promotes Epithelial–Mesenchymal Transition (EMT) in colorectal cancer through stabilisation of Src.

Peptide gels of fully-defined composition and mechanics for probing cell-cell and cell-matrix interactions in vitro (2019)
Journal Article
Ashworth, J. C., Thompson, J. L., James, J. R., Slater, C. E., Pijuan-Galitó, S., Lis-Slimak, K., …Merry, C. L. (2020). Peptide gels of fully-defined composition and mechanics for probing cell-cell and cell-matrix interactions in vitro. Matrix Biology, 85-86, 15-33. https://doi.org/10.1016/j.matbio.2019.06.009

© 2019 Current materials used for in vitro 3D cell culture are often limited by their poor similarity to human tissue, batch-to-batch variability and complexity of composition and manufacture. Here, we present a “blank slate” culture environment base... Read More about Peptide gels of fully-defined composition and mechanics for probing cell-cell and cell-matrix interactions in vitro.

Caffeine exposure induces browning features in adipose tissue in vitro and in vivo (2019)
Journal Article
Velickovic, K., Wayne, D., Lugo Leija, H. A., Bloor, I., Morris, D. E., Law, J., …Sottile, V. (2019). Caffeine exposure induces browning features in adipose tissue in vitro and in vivo. Scientific Reports, 9(1), Article 9104. https://doi.org/10.1038/s41598-019-45540-1

Brown adipose tissue (BAT) is able to rapidly generate heat and metabolise macronutrients, such as glucose and lipids, through activation of mitochondrial uncoupling protein 1 (UCP1). Diet can modulate UCP1 function but the capacity of individual nut... Read More about Caffeine exposure induces browning features in adipose tissue in vitro and in vivo.

Stand-alone intrathecal central nervous system (CNS) prophylaxis provide unclear benefit in reducing CNS relapse risk in elderly DLBCL patients treated with R-CHOP and is associated increased infection-related toxicity (2019)
Journal Article
Eyre, T. A., Kirkwood, A. A., Wolf, J., Hildyard, C., Mercer, C., Plaschkes, H., …Hatton, C. S. R. (2019). Stand-alone intrathecal central nervous system (CNS) prophylaxis provide unclear benefit in reducing CNS relapse risk in elderly DLBCL patients treated with R-CHOP and is associated increased infection-related toxicity. British Journal of Haematology, 187(2), 185-194. https://doi.org/10.1111/bjh.16070

Central nervous system (CNS) relapse following R-CHOP (rituximab, cyclophosphamide, doxorubicin, vincristine, prednisolone) occurs in 2–5% of patents with diffuse large B-cell lymphoma (DLBCL). Many patients aged ≥70 years are unsuitable for high-dos... Read More about Stand-alone intrathecal central nervous system (CNS) prophylaxis provide unclear benefit in reducing CNS relapse risk in elderly DLBCL patients treated with R-CHOP and is associated increased infection-related toxicity.