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Philanthotoxin analogues that selectively inhibit ganglionic nicotinic acetylcholine receptors with exceptional potency (2019)
Journal Article
Kachel, H. S., Franzyk, H., & Mellor, I. R. (2019). Philanthotoxin analogues that selectively inhibit ganglionic nicotinic acetylcholine receptors with exceptional potency. Journal of Medicinal Chemistry, 62(13), 6214-6222. https://doi.org/10.1021/acs.jmedchem.9b00519

Philanthotoxin-433 (PhTX-433) is an active component of the venom from the Egyptian digger wasp, Philanthus triangulum. PhTX-433 non-selectively inhibits several excitatory ligand-gated ion channels, and we recently showed that its synthetic analogue... Read More about Philanthotoxin analogues that selectively inhibit ganglionic nicotinic acetylcholine receptors with exceptional potency.

The roles of the human SETMAR (Metnase) protein in illegitimate DNA recombination and non-homologous end joining repair (2019)
Journal Article
Tellier, M., & Chalmers, R. (2019). The roles of the human SETMAR (Metnase) protein in illegitimate DNA recombination and non-homologous end joining repair. DNA Repair, 80, 26-35. https://doi.org/10.1016/j.dnarep.2019.06.006

SETMAR is a fusion between a SET-domain methyltransferase gene and a mariner-family transposase gene, which is specific to anthropoid primates. However, the ancestral SET gene is present in all other mammals and birds. SETMAR is reported to be involv... Read More about The roles of the human SETMAR (Metnase) protein in illegitimate DNA recombination and non-homologous end joining repair.

Cerebrovascular endothelial cells form transient Notch‐dependent cystic structures in zebrafish (2019)
Journal Article
Kugler, E. C., Lessen, M., Daetwyler, S., Chhabria, K., Savage, A. M., Silva, V., …Chico, T. J. (2019). Cerebrovascular endothelial cells form transient Notch‐dependent cystic structures in zebrafish. EMBO Reports, 20(8), https://doi.org/10.15252/embr.201847047

We identify a novel endothelial membrane behaviour in transgenic zebrafish. Cerebral blood vessels extrude large transient spherical structures that persist for an average of 23 min before regressing into the parent vessel. We term these structures "... Read More about Cerebrovascular endothelial cells form transient Notch‐dependent cystic structures in zebrafish.

Mediterranean diet adherence and cognitive function in older UK adults: The European Prospective Investigation into Cancer and Nutrition-Norfolk (EPIC-Norfolk) Study (2019)
Journal Article
Shannon, O. M., Stephan, B. C. M., Granic, A., Lentjes, M., Hayat, S., Mulligan, A., …Siervo, M. (2019). Mediterranean diet adherence and cognitive function in older UK adults: The European Prospective Investigation into Cancer and Nutrition-Norfolk (EPIC-Norfolk) Study. American Journal of Clinical Nutrition, 110(4), 938-948. https://doi.org/10.1093/ajcn/nqz114

© 2019 American Society for Nutrition. Background: In Mediterranean countries, adherence to a traditional Mediterranean dietary pattern (MedDiet) is associated with better cognitive function and reduced dementia risk. It is unclear if similar benefit... Read More about Mediterranean diet adherence and cognitive function in older UK adults: The European Prospective Investigation into Cancer and Nutrition-Norfolk (EPIC-Norfolk) Study.

The genetic basis of 3-hydroxypropanoate metabolism in Cupriavidus necator H16 (2019)
Journal Article
Arenas-López, C., Locker, J., Orol, D., Walter, F., Busche, T., Kalinowski, J., …Winzer, K. (2019). The genetic basis of 3-hydroxypropanoate metabolism in Cupriavidus necator H16. Biotechnology for Biofuels, 12, Article 150. https://doi.org/10.1186/s13068-019-1489-5

Background 3-Hydroxypropionic acid (3-HP) is a promising platform chemical with various industrial applications. Several metabolic routes to produce 3-HP from organic substrates such as sugars or glycerol have been implemented in yeast, enterobacter... Read More about The genetic basis of 3-hydroxypropanoate metabolism in Cupriavidus necator H16.

Spatiotemporally Super-Resolved Volumetric Traction Force Microscopy (2019)
Journal Article
Colin-York, H., Javanmardi, Y., Barbieri, L., Li, D., Korobchevskaya, K., Guo, Y., …Fritzsche, M. (2019). Spatiotemporally Super-Resolved Volumetric Traction Force Microscopy. Nano Letters, 19(7), 4427-4434. https://doi.org/10.1021/acs.nanolett.9b01196

Quantification of mechanical forces is a major challenge across biomedical sciences. Yet such measurements are essential to understanding the role of biomechanics in cell regulation and function. Traction force microscopy remains the most broadly app... Read More about Spatiotemporally Super-Resolved Volumetric Traction Force Microscopy.

Genome sequencing and transcriptome analyses of the Siberian hamster hypothalamus identify mechanisms for seasonal energy balance (2019)
Journal Article
Bao, R., Onishi, K. G., Tolla, E., Ebling, F. J. P., Lewis, J. E., Anderson, R. L., …Stevenson, T. J. (2019). Genome sequencing and transcriptome analyses of the Siberian hamster hypothalamus identify mechanisms for seasonal energy balance. Proceedings of the National Academy of Sciences, 116(26), 13116-13121. https://doi.org/10.1073/pnas.1902896116

Synthesis of triiodothyronine (T3) in the hypothalamus induces marked seasonal neuromorphology changes across taxa. How species-specific responses to T3 signaling in the CNS drive annual changes in body weight and energy balance remains uncharacteriz... Read More about Genome sequencing and transcriptome analyses of the Siberian hamster hypothalamus identify mechanisms for seasonal energy balance.

Adaptation processes that build CRISPR immunity: creative destruction, updated (2019)
Journal Article
Lau, C., Reeves, R., & Bolt, E. (2019). Adaptation processes that build CRISPR immunity: creative destruction, updated. Essays in Biochemistry, 63(2), 227-235. https://doi.org/10.1042/ebc20180073

Prokaryotes can defend themselves against invading mobile genetic elements (MGEs) by acquiring immune memory against them. The memory is a DNA database located at specific chromosomal sites called CRISPRs (clustered regularly interspaced short palind... Read More about Adaptation processes that build CRISPR immunity: creative destruction, updated.

RiboCas: A Universal CRISPR-Based Editing Tool for Clostridium (2019)
Journal Article
Cañadas, I. C., Groothuis, D., Zygouropoulou, M., Rodrigues, R., & Minton, N. P. (2019). RiboCas: A Universal CRISPR-Based Editing Tool for Clostridium. ACS Synthetic Biology, 8(6), 1379-1390. https://doi.org/10.1021/acssynbio.9b00075

Members of the genus Clostridium represent a diverse assemblage of species exhibiting both medical and industrial importance. Deriving both a greater understanding of their biology, while at the same time enhancing their exploitable properties, requi... Read More about RiboCas: A Universal CRISPR-Based Editing Tool for Clostridium.

Resolving the basal divisions in the stylommatophoran land snails and slugs with special emphasis on the position of the Scolodontidae (2019)
Journal Article
Saadi, A. J., & Wade, C. M. (2019). Resolving the basal divisions in the stylommatophoran land snails and slugs with special emphasis on the position of the Scolodontidae. Molecular Phylogenetics and Evolution, 139, Article 106529. https://doi.org/10.1016/j.ympev.2019.106529

The deep divisions at the base of the stylommatophoran land snails and slugs have proved to be controversial, with the phylogenetic position of the Scolodontidae remaining unresolved. Here we present a phylogenetic analysis of 34 stylommatophoran gen... Read More about Resolving the basal divisions in the stylommatophoran land snails and slugs with special emphasis on the position of the Scolodontidae.

A mutation in the endonuclease domain of mouse MLH3 reveals novel roles for MutLγ during crossover formation in meiotic prophase I (2019)
Journal Article
Toledo, M., Sun, X., Brieño-Enríquez, M. A., Raghavan, V., Gray, S., Pea, J., …Cohen, P. E. (2019). A mutation in the endonuclease domain of mouse MLH3 reveals novel roles for MutLγ during crossover formation in meiotic prophase I. PLoS Genetics, 15(6), 1-28. https://doi.org/10.1371/journal.pgen.1008177

During meiotic prophase I, double-strand breaks (DSBs) initiate homologous recombination leading to non-crossovers (NCOs) and crossovers (COs). In mouse, 10% of DSBs are designated to become COs, primarily through a pathway dependent on the MLH1-MLH3... Read More about A mutation in the endonuclease domain of mouse MLH3 reveals novel roles for MutLγ during crossover formation in meiotic prophase I.

Comparison of the ligand‐binding properties of fluorescent VEGF‐A isoforms to VEGF receptor 2 in living cells and membrane preparations using NanoBRET (2019)
Journal Article
Peach, C. J., Kilpatrick, L. E., Woolard, J., & Hill, S. J. (2019). Comparison of the ligand‐binding properties of fluorescent VEGF‐A isoforms to VEGF receptor 2 in living cells and membrane preparations using NanoBRET. British Journal of Pharmacology, 176(17), 3220-3235. https://doi.org/10.1111/bph.14755

Background and Purpose: Vascular Endothelial Growth Factor A (VEGF-A) is a key mediator of angiogenesis. A striking feature of the binding of a fluorescent analogue of VEGF165a to NanoLuciferase-tagged VEGF Receptor 2 (VEGFR2) in living cells is that... Read More about Comparison of the ligand‐binding properties of fluorescent VEGF‐A isoforms to VEGF receptor 2 in living cells and membrane preparations using NanoBRET.

Genome Sequence Analysis Reveals Selection Signatures in Endangered Trypanotolerant West African Muturu Cattle (2019)
Journal Article
Tijjani, A., Utsunomiya, Y. T., Ezekwe, A. G., Nashiru, O., & Hanotte, O. (2019). Genome Sequence Analysis Reveals Selection Signatures in Endangered Trypanotolerant West African Muturu Cattle. Frontiers in Genetics, 10, Article 442. https://doi.org/10.3389/fgene.2019.00442

Like most West African Bos taurus, the shorthorn Muturu is under threat of replacement or crossbreeding with zebu. Their populations are now reduced to a few hundred breeding individuals and they are considered endangered. So far, the genetic variati... Read More about Genome Sequence Analysis Reveals Selection Signatures in Endangered Trypanotolerant West African Muturu Cattle.

Crystal structures of the recombinant β-factor XIIa protease with bound Thr-Arg and Pro-Arg substrate mimetics (2019)
Journal Article
Pathak, M., Manna, R., Li, C., Kaira, B. G., Hamad, B. K., Belviso, B. D., …Emsley, J. (2019). Crystal structures of the recombinant β-factor XIIa protease with bound Thr-Arg and Pro-Arg substrate mimetics. Acta Crystallographica. Section d, Structural Biology, 75(6), 578-591. https://doi.org/10.1107/s2059798319006910

© 2019 International Union of Crystallography. Coagulation factor XII (FXII) is a key initiator of the contact pathway, which contributes to inflammatory pathways. FXII circulates as a zymogen, which when auto-activated forms factor XIIa (FXIIa). Her... Read More about Crystal structures of the recombinant β-factor XIIa protease with bound Thr-Arg and Pro-Arg substrate mimetics.

Generation of a fully erythromycin-sensitive strain of Clostridioides difficile using a novel CRISPR-Cas9 genome editing system (2019)
Journal Article
Ingle, P., Groothuis, D., Rowe, P., Huang, H., Cockayne, A., Kuehne, S. A., …Minton, N. P. (2019). Generation of a fully erythromycin-sensitive strain of Clostridioides difficile using a novel CRISPR-Cas9 genome editing system. Scientific Reports, 9, Article 8123. https://doi.org/10.1038/s41598-019-44458-y

© 2019, The Author(s). Understanding the molecular pathogenesis of Clostridioides difficile has relied on the use of ermB-based mutagens in erythromycin-sensitive strains. However, the repeated subcultures required to isolate sensitive variants can l... Read More about Generation of a fully erythromycin-sensitive strain of Clostridioides difficile using a novel CRISPR-Cas9 genome editing system.

Long‐term cardiovascular effects of vandetanib and pazopanib in normotensive rats (2019)
Journal Article
Cooper, S. L., Carter, J. J., March, J., & Woolard, J. (2019). Long‐term cardiovascular effects of vandetanib and pazopanib in normotensive rats. Pharmacology Research and Perspectives, 7(3), Article e00477. https://doi.org/10.1002/prp2.477

Vandetanib and pazopanib are clinically available, multi‐targeted inhibitors of vascular endothelial growth factor (VEGF) and platelet‐derived growth factor (PDGF) receptor tyrosine kinases. Short‐term VEGF receptor inhibition is associated with hype... Read More about Long‐term cardiovascular effects of vandetanib and pazopanib in normotensive rats.

First Report of a Novel Hepatozoon sp. in Giant Pandas (Ailuropoda melanoleuca) (2019)
Journal Article
Yu, J. H., Durrant, K. L., Liu, S., Carlin, E. P., Wang, C., Rodriguez, J., …Fleischer, R. C. (2019). First Report of a Novel Hepatozoon sp. in Giant Pandas (Ailuropoda melanoleuca). EcoHealth, 16(2), 338=345. https://doi.org/10.1007/s10393-019-01416-4

The first report of giant pandas (Ailuropoda melanoleuca) infected with a novel Hepatozoon species is presented. An intraleukocytic parasite was detected via routine blood smear from a zoo-housed giant panda at the National Zoological Park. Ribosomal... Read More about First Report of a Novel Hepatozoon sp. in Giant Pandas (Ailuropoda melanoleuca).

Theory and Experiments on Multi-Ion Permeation and Selectivity in the NaChBac Ion Channel (2019)
Journal Article
Gibby, W. A. T., Barabash, M. L., Guardiani, C., Luchinsky, D. G., Fedorenko, O. A., Roberts, S. K., & McClintock, P. V. E. (2019). Theory and Experiments on Multi-Ion Permeation and Selectivity in the NaChBac Ion Channel. Fluctuation and Noise Letters, 18(02), 1-13. https://doi.org/10.1142/s0219477519400078

The highly selective permeation of ions through biological ion channels is an unsolved problem of noise and fluctuations. In this paper, we motivate and introduce a non-equilibrium and self-consistent multi-species kinetic model, with the express aim... Read More about Theory and Experiments on Multi-Ion Permeation and Selectivity in the NaChBac Ion Channel.