Spatiotemporally Super-Resolved Volumetric Traction Force Microscopy

Colin-York, Huw; Javanmardi, Yousef; Barbieri, Liliana; Li, Di; Korobchevskaya, Kseniya; Guo, Yuting; Hall, Chloe; Taylor, Aaron; Khuon, Satya; Sheridan, Graham K.; Chew, Teng-Leong; Li, Dong; Moeendarbary, Emad; Fritzsche, Marco

Abstract

Quantification of mechanical forces is a major challenge across biomedical sciences. Yet such measurements are essential to understanding the role of biomechanics in cell regulation and function. Traction force microscopy remains the most broadly applied force probing technology but typically restricts itself to single-plane two-dimensional quantifications with limited spatiotemporal resolution. Here, we introduce an enhanced force measurement technique combining 3D super-resolution fluorescence structural illumination microscopy and traction force microscopy (3D-SIM-TFM) offering increased spatiotemporal resolution, opening-up unprecedented insights into physiological three-dimensional force production in living cells.

• Journal Article Type: Article
• Acceptance Date: Jun 14, 2019
• Online Publication Date: Jun 14, 2019
• Publication Date: Jul 10, 2019
• Deposit Date: Oct 22, 2019
• Publicly Available Date: Oct 24, 2019
• Journal: Nano Letters
• Print ISSN: 1530-6984
• Electronic ISSN: 1530-6992
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 19
• Issue: 7
• Pages: 4427-4434
• DOI: https://doi.org/10.1021/acs.nanolett.9b01196
• Public URL: https://nottingham-repository.worktribe.com/output/2889605
• Publisher URL: https://pubs.acs.org/doi/10.1021/acs.nanolett.9b01196