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Enzyme characterisation and kinetic modelling of the pentose phosphate pathway in yeast (2014)
Journal Article
Messiha, H. L., Kent, E., Malys, N., Carroll, K. M., Swainston, N., Mendes, P., & Smallbone, K. (2014). Enzyme characterisation and kinetic modelling of the pentose phosphate pathway in yeast. PeerJ, 2(e146v4), https://doi.org/10.7287/peerj.preprints.146v4

We present the quantification and kinetic characterisation of the enzymes of the pentose phosphate pathway in Saccharomyces cerevisiae. The data are combined into a mathematical model that describes the dynamics of this system and allows us to predic... Read More about Enzyme characterisation and kinetic modelling of the pentose phosphate pathway in yeast.

Ras GTPase-like protein MglA, a controller of bacterial social-motility in Myxobacteria, has evolved to control bacterial predation by Bdellovibrio (2014)
Journal Article
Milner, D. S., Till, R., Cadby, I., Lovering, A. L., Basford, S. M., Saxon, E. B., …Sockett, R. E. (2014). Ras GTPase-like protein MglA, a controller of bacterial social-motility in Myxobacteria, has evolved to control bacterial predation by Bdellovibrio. PLoS Genetics, 10(4), Article e1004253. https://doi.org/10.1371/journal.pgen.1004253

Bdellovibrio bacteriovorus invade Gram-negative bacteria in a predatory process requiring Type IV pili (T4P) at a single invasive pole, and also glide on surfaces to locate prey. Ras-like G-protein MglA, working with MglB and RomR in the deltaproteob... Read More about Ras GTPase-like protein MglA, a controller of bacterial social-motility in Myxobacteria, has evolved to control bacterial predation by Bdellovibrio.

Alcohol-related brain damage in humans (2014)
Journal Article
Quintas, L. E. M., Erdozain, A. M., Morentin, B., Bedford, L., King, E., Tooth, D., …Carter, W. G. (2014). Alcohol-related brain damage in humans. PLoS ONE, 9(4), Article e93586. https://doi.org/10.1371/journal.pone.0093586

Chronic excessive alcohol intoxications evoke cumulative damage to tissues and organs. We examined prefrontal cortex (Brodmann’s area (BA) 9) from 20 human alcoholics and 20 age, gender, and postmortem delay matched control subjects. H & E staining a... Read More about Alcohol-related brain damage in humans.

Cellular location and activity of Escherichia coli RecG proteins shed light on the function of its structurally unresolved C-terminus (2014)
Journal Article
Upton, A. L., Grove, J. I., Mahdi, A. A., Briggs, G. S., Milner, D. S., Rudolph, C. J., & Lloyd, R. G. (2014). Cellular location and activity of Escherichia coli RecG proteins shed light on the function of its structurally unresolved C-terminus. Nucleic Acids Research, 42(9), 5702-5714. https://doi.org/10.1093/nar/gku228

RecG is a DNA translocase encoded by most species of bacteria. The Escherichia coli protein targets branched DNA substrates and drives the unwinding and rewinding of DNA strands. Its ability to remodel replication forks and to genetically interact wi... Read More about Cellular location and activity of Escherichia coli RecG proteins shed light on the function of its structurally unresolved C-terminus.

The central cavity of ABCB1 undergoes alternating access during ATP hydrolysis (2014)
Journal Article
van Wonderen, J. H., McMahon, R. M., OMara, M. L., McDevitt, C. A., Thomson, A. J., Kerr, I. D., …Callaghan, R. (2014). The central cavity of ABCB1 undergoes alternating access during ATP hydrolysis. FEBS Journal, 281(9), 2190-2201. https://doi.org/10.1111/febs.12773

Understanding the process that underlies multidrug recognition and efflux by P-glycoprotein (ABCB1) remains a key biological challenge. Structural data have recently become available for the murine and Caenorhabditis elegans homologues of ABCB1; howe... Read More about The central cavity of ABCB1 undergoes alternating access during ATP hydrolysis.

PLA/chitosan/keratin composites for biomedical applications (2014)
Journal Article
Tanase, C. E., & Spiridon, I. (2014). PLA/chitosan/keratin composites for biomedical applications. Materials Science and Engineering: C, 40, 242-247. https://doi.org/10.1016/j.msec.2014.03.054

Novel composites based on PLA, chitosan and keratin was obtained via blend preparation. The goal of this contribution was to evaluate mechanical and in vitro behavior of the composites. The results point out composites with improved Young modulus and... Read More about PLA/chitosan/keratin composites for biomedical applications.

Phase variation mediates reductions in expression of surface proteins during persistent meningococcal carriage (2014)
Journal Article
Alamro, M., Bidmos, F. A., Chan, H., Oldfield, N. J., Newton, E., Bai, X., …Bayliss, C. D. (2014). Phase variation mediates reductions in expression of surface proteins during persistent meningococcal carriage. Infection and Immunity, 82(6), 2472-2484. https://doi.org/10.1128/IAI.01521-14

Asymptomatic and persistent colonization of the upper respiratory tract by Neisseria meningitidis occurs despite elicitation of adaptive immune responses against surface antigens. A putative mechanism for facilitating host persistence of this bacteri... Read More about Phase variation mediates reductions in expression of surface proteins during persistent meningococcal carriage.

One to rule them all: A highly conserved motif in mariner transposase controls multiple steps of transposition (2014)
Journal Article
Bouuaert, C. C., Chalmers, R., & Tellier, M. (2014). One to rule them all: A highly conserved motif in mariner transposase controls multiple steps of transposition. Mobile Genetic Elements, 4(2), Article e28807. https://doi.org/10.4161/mge.28807

The development of transposon-based genome manipulation tools can benefit greatly from understanding transposons’ inherent regulatory mechanisms. The Tc1-mariner transposons, which are being widely used in biotechnological applications, are subject t... Read More about One to rule them all: A highly conserved motif in mariner transposase controls multiple steps of transposition.

Snacks containing whey protein and polydextrose induce a sustained reduction in daily energy intake over 2 wk under free-living conditions (2014)
Journal Article
MacDonald, I., Astbury, N. M., Taylor, M. A., & French, S. J. (2014). Snacks containing whey protein and polydextrose induce a sustained reduction in daily energy intake over 2 wk under free-living conditions. American Journal of Clinical Nutrition, 99(5), 1131-1140. https://doi.org/10.3945/ajcn.113.075978

Background: The manipulation of the composition of foods consumed as between-meal snacks may aid daily energy restriction. Objectives: We compared the effects of the consumption of 2 energymatched snack bars on appetite, energy intake (EI), and metab... Read More about Snacks containing whey protein and polydextrose induce a sustained reduction in daily energy intake over 2 wk under free-living conditions.

Diversification and repeated morphological transitions in endoparasitic cnidarians (Myxozoa: Malacosporea) (2014)
Journal Article
Hartikainen, H., Gruhl, A., & Okamura, B. (2014). Diversification and repeated morphological transitions in endoparasitic cnidarians (Myxozoa: Malacosporea). Molecular Phylogenetics and Evolution, 76(1), 261-269. https://doi.org/10.1016/j.ympev.2014.03.010

Malacosporeans are a poorly known myxozoan clade that uniquely demonstrates a tissue level of organisation. Thus, when exploiting their invertebrate hosts (freshwater bryozoans) they occur as non-motile sacs or vermiform stages capable of active swim... Read More about Diversification and repeated morphological transitions in endoparasitic cnidarians (Myxozoa: Malacosporea).

Mikrocytids Are a Broadly Distributed and Divergent Radiation of Parasites in Aquatic Invertebrates (2014)
Journal Article
Hartikainen, H., Stentiford, G., Bateman, K., Berney, C., Feist, S., Longshaw, M., …Bass, D. (2014). Mikrocytids Are a Broadly Distributed and Divergent Radiation of Parasites in Aquatic Invertebrates. Current Biology, 24(7), 807-812. https://doi.org/10.1016/j.cub.2014.02.033

Microcell parasites have independently evolved in several eukaryotic lineages and are increasingly recognized as important and emerging pathogens of diverse hosts, including species of economic importance subject to international legislation concerni... Read More about Mikrocytids Are a Broadly Distributed and Divergent Radiation of Parasites in Aquatic Invertebrates.

Paget disease of bone-associated UBA domain mutations of SQSTM1 exert distinct effects on protein structure and function (2014)
Journal Article
Goode, A., Long, J. E., Shaw, B., Ralston, S. H., Visconti, M. R., Gianfrancesco, F., …Layfield, R. (2014). Paget disease of bone-associated UBA domain mutations of SQSTM1 exert distinct effects on protein structure and function. BBA - Molecular Basis of Disease, 1842(7), 992-1000. https://doi.org/10.1016/j.bbadis.2014.03.006

SQSTM1 mutations are common in patients with Paget disease of bone (PDB), with most affecting the C-terminal ubiquitin-associated (UBA) domain of the SQSTM1 protein. We performed structural and functional analyses of two UBA domain mutations, an I424... Read More about Paget disease of bone-associated UBA domain mutations of SQSTM1 exert distinct effects on protein structure and function.

The oxidative inactivation of FeFe hydrogenase reveals the flexibility of the H-cluster (2014)
Journal Article
Fourmond, V., Greco, C., Sybirna, K., Baffert, C., Wang, P. H., Ezanno, P., …Léger, C. (2014). The oxidative inactivation of FeFe hydrogenase reveals the flexibility of the H-cluster. Nature Chemistry, 6(4), 336-342. https://doi.org/10.1038/nchem.1892

Nature is a valuable source of inspiration in the design of catalysts, and various approaches are used to elucidate the mechanism of hydrogenases, the enzymes that oxidize or produce H 2. In FeFe hydrogenases, H 2 oxidation occurs at the H-cluster, a... Read More about The oxidative inactivation of FeFe hydrogenase reveals the flexibility of the H-cluster.

Immunocompetent 3D model of human upper airway for disease modeling and in vitro drug evaluation (2014)
Journal Article
Harrington, H., Cato, P., Salazar, F., Wilkinson, M., Knox, A., Haycock, J. W., …Ghaemmaghami, A. M. (2014). Immunocompetent 3D model of human upper airway for disease modeling and in vitro drug evaluation. Molecular Pharmaceutics, 11(7), 2082-2091. https://doi.org/10.1021/mp5000295

The development of more complex in vitro models for the assessment of novel drugs and chemicals is needed because of the limited biological relevance of animal models to humans as well as ethical considerations. Although some human-cell-based assays... Read More about Immunocompetent 3D model of human upper airway for disease modeling and in vitro drug evaluation.

Targeting Staphylococcus aureus quorum sensing with nonpeptidic small molecule inhibitors (2014)
Journal Article
Murray, E. J., Crowley, R. C., Truman, A., Clarke, S. R., Cottam, J. A., Jadhav, G. P., …Williams, P. (2014). Targeting Staphylococcus aureus quorum sensing with nonpeptidic small molecule inhibitors. Journal of Medicinal Chemistry, 57(6), 2813-2819. https://doi.org/10.1021/jm500215s

A series of 3-oxo-C 12 -HSL, tetramic acid, and tetronic acid analogues were synthesized to gain insights into the structural requirements for quorum sensing inhibition in Staphylococcus aureus. Compounds active against agr were noncompetitive inhibi... Read More about Targeting Staphylococcus aureus quorum sensing with nonpeptidic small molecule inhibitors.

Crosstalk between transposase subunits during cleavage of the mariner transposon (2014)
Journal Article
Claeys Bouuaert, C., Walker, N., Liu, D., & Chalmers, R. (2014). Crosstalk between transposase subunits during cleavage of the mariner transposon. Nucleic Acids Research, 42(9), https://doi.org/10.1093/nar/gku172

Mariner transposition is a complex reaction that involves three recombination sites and six strand breaking and joining reactions. This requires precise spatial and temporal coordination between the different components to ensure a productive outcome... Read More about Crosstalk between transposase subunits during cleavage of the mariner transposon.

Natural and disease-specific autoantibodies in chronic obstructive pulmonary disease (2014)
Journal Article
Daffa, N., Tighe, P. J., Corne, J., Fairclough, L. C., & Todd, I. (2014). Natural and disease-specific autoantibodies in chronic obstructive pulmonary disease. Clinical and Experimental Immunology, 180(1), https://doi.org/10.1111/cei.12565

Autoimmunity may contribute to the pathogenesis of chronic obstructive pulmonary disease (COPD). Studies have identified disease-specific autoantibodies (DSAAbs) in COPD patients, but natural autoantibodies (NAAbs) may also play a role. Previous stud... Read More about Natural and disease-specific autoantibodies in chronic obstructive pulmonary disease.

High-content screening identifies small molecules that remove nuclear foci, affect MBNL distribution and CELF1 protein levels via a PKC-independent pathway in myotonic dystrophy cell lines (2014)
Journal Article
Ketley, A., Chen, C. Z., Li, X., Arya, S., Robinson, T. E., Granados-Riveron, J. T., …Brook, J. D. (2014). High-content screening identifies small molecules that remove nuclear foci, affect MBNL distribution and CELF1 protein levels via a PKC-independent pathway in myotonic dystrophy cell lines. Human Molecular Genetics, 23(6), https://doi.org/10.1093/hmg/ddt542

Myotonic dystrophy (DM) is a multi-system neuromuscular disorder for which there is no treatment. We have developed a medium throughput phenotypic assay, based on the identification of nuclear foci in DM patient cell lines using in situ hybridization... Read More about High-content screening identifies small molecules that remove nuclear foci, affect MBNL distribution and CELF1 protein levels via a PKC-independent pathway in myotonic dystrophy cell lines.