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Enzyme characterisation and kinetic modelling of the pentose phosphate pathway in yeast

Messiha, Hanan L.; Kent, Edward; Malys, Naglis; Carroll, Kathleen M.; Swainston, Neil; Mendes, Pedro; Smallbone, Kieran

Authors

Hanan L. Messiha

Edward Kent

Kathleen M. Carroll

Neil Swainston

Pedro Mendes

Kieran Smallbone



Abstract

We present the quantification and kinetic characterisation of the enzymes of the pentose phosphate pathway in Saccharomyces cerevisiae. The data are combined into a mathematical model that describes the dynamics of this system and allows us to predict changes in metabolite concentrations and fluxes in response to perturbations. We use the model to study the response of yeast to a glucose pulse. We then combine the model with an existing glycolysis model to study the effect of oxidative stress on carbohydrate metabolism. The combination of these two models was made possible by the standardised enzyme kinetic experiments carried out in both studies. This work demonstrates the feasibility of constructing larger network-scale models by merging smaller pathway-scale models.

Citation

Messiha, H. L., Kent, E., Malys, N., Carroll, K. M., Swainston, N., Mendes, P., & Smallbone, K. (2014). Enzyme characterisation and kinetic modelling of the pentose phosphate pathway in yeast. PeerJ, 2(e146v4), doi:10.7287/peerj.preprints.146v4

Journal Article Type Article
Acceptance Date Apr 10, 2014
Publication Date Apr 10, 2014
Deposit Date Jul 6, 2016
Publicly Available Date Jul 6, 2016
Journal PeerJ
Electronic ISSN 2167-8359
Publisher PeerJ
Peer Reviewed Not Peer Reviewed
Volume 2
Issue e146v4
DOI https://doi.org/10.7287/peerj.preprints.146v4
Public URL http://eprints.nottingham.ac.uk/id/eprint/34654
Publisher URL https://peerj.com/preprints/146v4/
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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