Epigallocatechin-3-gallate Inhibits Lactase but Is Alleviated by Salivary Proline-Rich Proteins

Naz, Shahina; Siddiqi, Rahmanullah; Dew, Tristan P.; Williamson, Gary

doi:10.1021/jf103072z

Untargeted metabolomic profiling of 100% malt beers versus those containing barley adjunct (2024)
Journal Article
Yorke, J., Dew, T., & Cook, D. (2024). Untargeted metabolomic profiling of 100% malt beers versus those containing barley adjunct. Journal of the Institute of Brewing, 130(1), 31-46. https://doi.org/10.58430/jib.v130i1.46

Why was the work done: The incorporation of unmalted barley at high proportion in the grist can introduce unfavourable bitter and astringent characteristics to beer, resulting in an upper limit on the proportion used. The flavour active compounds fro... Read More about Untargeted metabolomic profiling of 100% malt beers versus those containing barley adjunct.

Coordination between microbiota and root endodermis supports plant mineral nutrient homeostasis (2020)
Journal Article
Salas-González, I., Reyt, G., Flis, P., Custódio, V., Gopaulchan, D., Bakhoum, N., …Castrillo, G. (2021). Coordination between microbiota and root endodermis supports plant mineral nutrient homeostasis. Science, 371(6525), Article eabd0695. https://doi.org/10.1126/science.abd0695

Copyright © 2021, American Association for the Advancement of Science. Plant roots and animal guts have evolved specialized cell layers to control mineral nutrient homeostasis. These layers must tolerate the resident microbiota while keeping homeosta... Read More about Coordination between microbiota and root endodermis supports plant mineral nutrient homeostasis.

Differential effects of resveratrol on the dilator responses of femoral arteries, ex vivo (2019)
Journal Article
Diaz, M., Parikh, V., Ismail, S., Maxamed, R., Tye, E., Austin, C., …Azzawi, M. (2019). Differential effects of resveratrol on the dilator responses of femoral arteries, ex vivo. Nitric Oxide: Biology and Chemistry, 92, 1-10. https://doi.org/10.1016/j.niox.2019.07.008

Resveratrol is a plant-derived phytoalexin with antioxidant, anti-inflammatory and cardio-protective properties and may be a promising therapeutic intervention strategy in cardiovascular disease. Here, we investigated the acute direct effects of tran... Read More about Differential effects of resveratrol on the dilator responses of femoral arteries, ex vivo.

Oral green tea catechins do not provide photoprotection from direct DNA damage induced by higher dose solar simulated radiation: A randomized controlled trial (2018)
Journal Article
Farrar, M. D., Huq, R., Mason, S., Nicolaou, A., Clarke, K. A., Dew, T. P., …Rhodes, L. E. (2018). Oral green tea catechins do not provide photoprotection from direct DNA damage induced by higher dose solar simulated radiation: A randomized controlled trial. Journal of The American Academy of Dermatology, 78(2), 414-416. https://doi.org/10.1016/j.jaad.2017.08.021

Green tea catechins and their metabolites in human skin before and after exposure to ultraviolet radiation (2015)
Journal Article
Clarke, K. A., Dew, T. P., Watson, R. E., Farrar, M. D., Osman, J. E., Nicolaou, A., …Williamson, G. (2016). Green tea catechins and their metabolites in human skin before and after exposure to ultraviolet radiation. Journal of Nutritional Biochemistry, 27, 203-210. https://doi.org/10.1016/j.jnutbio.2015.09.001

Dietary flavonoids may protect against sunburn inflammation in skin. Preliminary reports using less complete analysis suggest that certain catechins and their metabolites are found in skin biopsies and blister fluid after consumption of green tea; ho... Read More about Green tea catechins and their metabolites in human skin before and after exposure to ultraviolet radiation.

The effects of chronic trans-resveratrol supplementation on aspects of cognitive function, mood, sleep, health and cerebral blood flow in healthy, young humans (2015)
Journal Article
Wightman, E. L., Haskell-Ramsay, C. F., Reay, J. L., Williamson, G., Dew, T., Zhang, W., & Kennedy, D. O. (2015). The effects of chronic trans-resveratrol supplementation on aspects of cognitive function, mood, sleep, health and cerebral blood flow in healthy, young humans. British Journal of Nutrition, 114(9), 1427-1437. https://doi.org/10.1017/s0007114515003037

Single doses of resveratrol have previously been shown to increase cerebral blood flow (CBF) with no clear effect on cognitive function or mood in healthy adults. Chronic resveratrol consumption may increase the poor bioavailability of resveratrol or... Read More about The effects of chronic trans-resveratrol supplementation on aspects of cognitive function, mood, sleep, health and cerebral blood flow in healthy, young humans.

A randomized controlled trial of green tea catechins in protection against ultraviolet radiation–induced cutaneous inflammation (2015)
Journal Article
Farrar, M. D., Nicolaou, A., Clarke, K. A., Mason, S., Massey, K. A., Dew, T. P., …Rhodes, L. E. (2015). A randomized controlled trial of green tea catechins in protection against ultraviolet radiation–induced cutaneous inflammation. American Journal of Clinical Nutrition, 102(3), 608-615. https://doi.org/10.3945/ajcn.115.107995

Background: Safe systemic protection from the health hazards of ultraviolet radiation (UVR) in sunlight is desirable. Green tea is consumed globally and is reported to have anti-inflammatory properties, which may be mediated through the impact on cyc... Read More about A randomized controlled trial of green tea catechins in protection against ultraviolet radiation–induced cutaneous inflammation.

Gastrointestinal absorption and metabolism of hesperetin-7-O-rutinoside and hesperetin-7-O-glucoside in healthy humans (2015)
Journal Article
Actis-Goretta, L., Dew, T. P., Lévèques, A., Pereira-Caro, G., Rein, M., Teml, A., …Williamson, G. (2015). Gastrointestinal absorption and metabolism of hesperetin-7-O-rutinoside and hesperetin-7-O-glucoside in healthy humans. Molecular Nutrition and Food Research, 59(9), 1651-1662. https://doi.org/10.1002/mnfr.201500202

Scope
Hesperetin‐7‐O ‐rutinoside (hesperidin) reduces blood pressure in healthy volunteers but its intestinal absorption and metabolism are not fully understood. Therefore, we aimed to determine sites of absorption and metabolism of dietary flavanon... Read More about Gastrointestinal absorption and metabolism of hesperetin-7-O-rutinoside and hesperetin-7-O-glucoside in healthy humans.

High performance liquid chromatography tandem mass spectrometry dual extraction method for identification of green tea catechin metabolites excreted in human urine (2014)
Journal Article
Clarke, K. A., Dew, T. P., Watson, R. E., Farrar, M. D., Bennett, S., Nicolaou, A., …Williamson, G. (2014). High performance liquid chromatography tandem mass spectrometry dual extraction method for identification of green tea catechin metabolites excreted in human urine. Journal of Chromatography B, 972, 29-37. https://doi.org/10.1016/j.jchromb.2014.09.035

The simultaneous analysis of free-form and conjugated flavonoids in the same sample is difficult but necessary to properly estimate their bioavailability. A method was developed to optimise the extraction of both free and conjugated forms of catechin... Read More about High performance liquid chromatography tandem mass spectrometry dual extraction method for identification of green tea catechin metabolites excreted in human urine.

Effects of resveratrol alone or in combination with piperine on cerebral blood flow parameters and cognitive performance in human subjects: a randomised, double-blind, placebo-controlled, cross-over investigation (2014)
Journal Article
Wightman, E. L., Reay, J. L., Haskell, C. F., Williamson, G., Dew, T. P., & Kennedy, D. O. (2014). Effects of resveratrol alone or in combination with piperine on cerebral blood flow parameters and cognitive performance in human subjects: a randomised, double-blind, placebo-controlled, cross-over investigation. British Journal of Nutrition, 112(2), 203-213. https://doi.org/10.1017/s0007114514000737

Previous research has shown that resveratrol can increase cerebral blood flow (CBF) in the absence of improved cognitive performance in healthy, young human subjects during the performance of cognitively demanding tasks. This lack of cognitive effect... Read More about Effects of resveratrol alone or in combination with piperine on cerebral blood flow parameters and cognitive performance in human subjects: a randomised, double-blind, placebo-controlled, cross-over investigation.

Urinary metabolite profiling identifies novel colonic metabolites and conjugates of phenolics in healthy volunteers (2014)
Journal Article
Pimpão, R. C., Dew, T., Figueira, M. E., McDougall, G. J., Stewart, D., Ferreira, R. B., …Williamson, G. (2014). Urinary metabolite profiling identifies novel colonic metabolites and conjugates of phenolics in healthy volunteers. Molecular Nutrition and Food Research, 58(7), 1414-1425. https://doi.org/10.1002/mnfr.201300822

Scope
The colonic metabolism of dietary flavonoids, phenolic acids and their phenolic metabolites is complex and many metabolites and conjugates have not yet been unambiguously identified in humans.

Methods and results
Urine samples from nine he... Read More about Urinary metabolite profiling identifies novel colonic metabolites and conjugates of phenolics in healthy volunteers.

Controlled flax interventions for the improvement of menopausal symptoms and postmenopausal bone health: a systematic review (2013)
Journal Article
Dew, T. P., & Williamson, G. (2013). Controlled flax interventions for the improvement of menopausal symptoms and postmenopausal bone health: a systematic review. Menopause, 20(11), 1207-1215. https://doi.org/10.1097/gme.0b013e3182896ae5

Concerns regarding hormone therapy safety have led to interest in the use of phytoestrogens for a variety of menopause-related health complaints. Recent meta-analyses concerning soy and postmenopausal bone mineral density, flax and serum cholesterol... Read More about Controlled flax interventions for the improvement of menopausal symptoms and postmenopausal bone health: a systematic review.

Urinary excretion of ginkgolide terpene lactones following acute consumption ofGinkgo bilobaextract: Dose-Dependent Urinary Excretion of Ginkgolides (2013)
Journal Article
Dew, T. P., Wang, G., & Williamson, G. (2014). Urinary excretion of ginkgolide terpene lactones following acute consumption ofGinkgo bilobaextract: Dose-Dependent Urinary Excretion of Ginkgolides. BioFactors, 40(2), 268-274. https://doi.org/10.1002/biof.1151

Urinary biomarkers of plant food supplement (PFS) exposure/intake represent an accurate, objective tool for determining PFS consumption in humans with applications ranging from epidemiology to subject compliance in clinical trials. Ginkgo biloba rema... Read More about Urinary excretion of ginkgolide terpene lactones following acute consumption ofGinkgo bilobaextract: Dose-Dependent Urinary Excretion of Ginkgolides.

Analysis of Phenolic Compounds in Portuguese Wild and Commercial Berries after Multienzyme Hydrolysis (2013)
Journal Article
Pimpão, R. C., Dew, T., Oliveira, P. B., Williamson, G., Ferreira, R. B., & Santos, C. N. (2013). Analysis of Phenolic Compounds in Portuguese Wild and Commercial Berries after Multienzyme Hydrolysis. Journal of Agricultural and Food Chemistry, 61(17), 4053-4062. https://doi.org/10.1021/jf305498j

Berry fruits are a good source of phenolic compounds and thus, potentially beneficial to health. Phenolic compounds are mainly present as a variety of conjugated forms, either with sugars via O-glycosidic bonds or with other polyols as esters. This c... Read More about Analysis of Phenolic Compounds in Portuguese Wild and Commercial Berries after Multienzyme Hydrolysis.

Oral green tea catechin metabolites are incorporated into human skin and protect against UV radiation-induced cutaneous inflammation in association with reduced production of pro-inflammatory eicosanoid 12-hydroxyeicosatetraenoic acid (2013)
Journal Article
Rhodes, L. E., Darby, G., Massey, K. A., Clarke, K. A., Dew, T. P., Farrar, M. D., …Nicolaou, A. (2013). Oral green tea catechin metabolites are incorporated into human skin and protect against UV radiation-induced cutaneous inflammation in association with reduced production of pro-inflammatory eicosanoid 12-hydroxyeicosatetraenoic acid. British Journal of Nutrition, 110(5), 891-900. https://doi.org/10.1017/s0007114512006071

Green tea catechins (GTC) reduce UV radiation (UVR)-induced inflammation in experimental models, but human studies are scarce and their cutaneous bioavailability and mechanism of photoprotection are unknown. We aimed to examine oral GTC cutaneous upt... Read More about Oral green tea catechin metabolites are incorporated into human skin and protect against UV radiation-induced cutaneous inflammation in association with reduced production of pro-inflammatory eicosanoid 12-hydroxyeicosatetraenoic acid.

Predicting Phenolic Acid Absorption in Caco-2 Cells: A Theoretical Permeability Model and Mechanistic Study (2011)
Journal Article
Farrell, T. L., Poquet, L., Dew, T. P., Barber, S., & Williamson, G. (2012). Predicting Phenolic Acid Absorption in Caco-2 Cells: A Theoretical Permeability Model and Mechanistic Study. Drug Metabolism and Disposition, 40(2), 397-406. https://doi.org/10.1124/dmd.111.041665

There is a considerable need to rationalize the membrane permeability and mechanism of transport for potential nutraceuticals. The aim of this investigation was to develop a theoretical permeability equation, based on a reported descriptive absorptio... Read More about Predicting Phenolic Acid Absorption in Caco-2 Cells: A Theoretical Permeability Model and Mechanistic Study.

Absorption and Metabolism of Chlorogenic Acids in Cultured Gastric Epithelial Monolayers (2011)
Journal Article
Farrell, T. L., Dew, T. P., Poquet, L., Hanson, P., & Williamson, G. (2011). Absorption and Metabolism of Chlorogenic Acids in Cultured Gastric Epithelial Monolayers. Drug Metabolism and Disposition, 39(12), 2338-2346. https://doi.org/10.1124/dmd.111.040147

Gastric absorption of feruloylquinic acid and di-O-caffeoylquinic acid analogs has never been investigated despite their potential contribution to the proposed beneficial health effects leading to reduced risk of type 2 diabetes. Using a cultured gas... Read More about Absorption and Metabolism of Chlorogenic Acids in Cultured Gastric Epithelial Monolayers.

Review of the efficacy of green tea, isoflavones and aloe vera supplements based on randomised controlled trials (2011)
Journal Article
Williamson, G., Coppens, P., Serra-Majem, L., & Dew, T. (2011). Review of the efficacy of green tea, isoflavones and aloe vera supplements based on randomised controlled trials. Food and Function, 2(12), 753-759. https://doi.org/10.1039/c1fo10101c

We assess the evidence for health benefits of three commonly consumed plant food supplements (PFS), green tea, isoflavone and aloe vera, based on published systematic reviews of randomised controlled trials (RCTs). Whilst the potential benefits of gr... Read More about Review of the efficacy of green tea, isoflavones and aloe vera supplements based on randomised controlled trials.

Influence of Combined Biotic and Abiotic Stress on Nutritional Quality Parameters in Tomato (Solanum lycopersicum) (2011)
Journal Article
Atkinson, N. J., Dew, T. P., Orfila, C., & Urwin, P. E. (2011). Influence of Combined Biotic and Abiotic Stress on Nutritional Quality Parameters in Tomato (Solanum lycopersicum). Journal of Agricultural and Food Chemistry, 59(17), 9673-9682. https://doi.org/10.1021/jf202081t

Induction of abiotic stress in tomato plants has been proposed as a mechanism for improving the nutritional quality of fruits. However, the occurrence of biotic stress can interfere with normal abiotic stress responses. In this study, the combined ef... Read More about Influence of Combined Biotic and Abiotic Stress on Nutritional Quality Parameters in Tomato (Solanum lycopersicum).

Epigallocatechin-3-gallate Inhibits Lactase but Is Alleviated by Salivary Proline-Rich Proteins (2011)
Journal Article
Naz, S., Siddiqi, R., Dew, T. P., & Williamson, G. (2011). Epigallocatechin-3-gallate Inhibits Lactase but Is Alleviated by Salivary Proline-Rich Proteins. Journal of Agricultural and Food Chemistry, 59(6), 2734-2738. https://doi.org/10.1021/jf103072z

Lactase phlorizin hydrolase is a small intestinal brush border enzyme that catalyzes the hydrolysis of the milk sugar, lactose, and also many flavonoid glucosides. We demonstrate that epigallocatechin-3-gallate (EGCG), the principal flavonoid from gr... Read More about Epigallocatechin-3-gallate Inhibits Lactase but Is Alleviated by Salivary Proline-Rich Proteins.

All Outputs (22)