Green tea catechins and their metabolites in human skin before and after exposure to ultraviolet radiation

Clarke, Kayleigh A.; Dew, Tristan P.; Watson, Rachel E.B.; Farrar, Mark D.; Osman, Joanne E.; Nicolaou, Anna; Rhodes, Lesley E.; Williamson, Gary

Abstract

Dietary flavonoids may protect against sunburn inflammation in skin. Preliminary reports using less complete analysis suggest that certain catechins and their metabolites are found in skin biopsies and blister fluid after consumption of green tea; however, it is not known if they are affected by solar-simulated ultraviolet radiation (UVR) or whether conjugated forms, with consequently altered bioactivity, are present. The present study tested the hypothesis that UVR affects the catechin levels in the skin of healthy volunteers after consumption of green tea and how catechins in the plasma are related to their presence in skin tissue samples. In an open oral intervention study, 11 subjects consumed green tea and vitamin C supplements daily for 3 months. Presupplementation and postsupplementation plasma samples, suction blister fluid and skin biopsies were collected; the latter two samples were collected both before and after UVR. A sensitive high-performance liquid chromatography/mass spectrometric assay was used to measure the intact catechin metabolites, conjugates and free forms. Seven green tea catechins and their corresponding metabolites were identified postsupplementation in skin biopsies, 20 in blister fluid and 26 in plasma, with 15 green tea catechin metabolites present in both blister fluid and plasma. The valerolactone, O-methyl-M4-O-sulfate, a gut microbiota metabolite of catechins, was significantly increased 1.6-fold by UVR in blister fluid samples. In conclusion, there were some common catechin metabolites in the plasma and blister fluid, and the concentration was always higher in plasma. The results suggest that green tea catechins and metabolites are bioavailable in skin and provide a novel link between catechin metabolites derived from the skin and gut microbiota.

Citation

Clarke, K. A., Dew, T. P., Watson, R. E., Farrar, M. D., Osman, J. E., Nicolaou, A., …Williamson, G. (2016). Green tea catechins and their metabolites in human skin before and after exposure to ultraviolet radiation. Journal of Nutritional Biochemistry, 27, 203-210. https://doi.org/10.1016/j.jnutbio.2015.09.001

• Journal Article Type: Article
• Acceptance Date: Sep 3, 2015
• Online Publication Date: Sep 12, 2015
• Publication Date: 2016-01
• Deposit Date: Aug 3, 2020
• Publicly Available Date: Aug 4, 2020
• Journal: The Journal of Nutritional Biochemistry
• Print ISSN: 0955-2863
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 27
• Pages: 203-210
• DOI: https://doi.org/10.1016/j.jnutbio.2015.09.001
• Public URL: https://nottingham-repository.worktribe.com/output/4677796
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0955286315002363?via%3Dihub
• Additional Information: This article is maintained by: Elsevier; Article Title: Green tea catechins and their metabolites in human skin before and after exposure to ultraviolet radiation; Journal Title: The Journal of Nutritional Biochemistry; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.jnutbio.2015.09.001; Content Type: article; Copyright: Copyright © 2015 The Authors. Published by Elsevier Inc.