Differential effects of resveratrol on the dilator responses of femoral arteries, ex vivo

Diaz, Miguel; Parikh, Vijay; Ismail, Saira; Maxamed, Raja; Tye, Emily; Austin, Clare; Dew, Tristan; Graf, Brigitte A.; Vanhees, Luc; Degens, Hans; Azzawi, May

Abstract

Resveratrol is a plant-derived phytoalexin with antioxidant, anti-inflammatory and cardio-protective properties and may be a promising therapeutic intervention strategy in cardiovascular disease. Here, we investigated the acute direct effects of trans-resveratrol (RV), on acetylcholine (ACh)-induced and flow-mediated dilation (FMD) of isolated pressurized femoral arteries of young (4-month-old) and old (26-month-old) mice. Vessel exposure to RV enhanced ACh (0.01–1.0 mM)-induced dilation (p  less than 0.05), but not FMD (@ 5–10 μL⋅min−1) (p less than 0.05) in both young and old mice. After RV incubation, acute nitric oxide (NO) production by cultured endothelial cells was increased in response to 0.01 mM ACh, but reduced by flow (5–10 μL⋅min−1; p  less than 0.05). In isolated femoral arteries from endothelial nitric oxide synthase knockout (eNOS−/-) mice, RV had no overall effect on FMD, but potentiated ACh induced dilation, that was completely abolished by potassium channel blockers, Apamin and Tram 34 (p less than 0.01). We demonstrate that the non-metabolised form of RV stimulates ACh-induced dilation via the NO and EDHF pathways, but not FMD by interaction with the cyclo-oxygenase pathway. Our findings have important implications in the use of RV (for both young and aged) under ‘normal’ non-diseased physiological states.

Citation

Diaz, M., Parikh, V., Ismail, S., Maxamed, R., Tye, E., Austin, C., …Azzawi, M. (2019). Differential effects of resveratrol on the dilator responses of femoral arteries, ex vivo. Nitric Oxide: Biology and Chemistry, 92, 1-10. https://doi.org/10.1016/j.niox.2019.07.008

• Journal Article Type: Article
• Acceptance Date: Jul 25, 2019
• Online Publication Date: Jul 29, 2019
• Publication Date: Nov 1, 2019
• Deposit Date: Aug 3, 2020
• Publicly Available Date: Aug 4, 2020
• Journal: Nitric Oxide
• Print ISSN: 1089-8603
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 92
• Pages: 1-10
• DOI: https://doi.org/10.1016/j.niox.2019.07.008
• Keywords: Clinical Biochemistry; Biochemistry; Cancer Research; Physiology
• Public URL: https://nottingham-repository.worktribe.com/output/4677684
• Publisher URL: https://www.sciencedirect.com/science/article/abs/pii/S1089860319301119?via%3Dihub