Predicting Phenolic Acid Absorption in Caco-2 Cells: A Theoretical Permeability Model and Mechanistic Study

Farrell, Tracy L.; Poquet, Laure; Dew, Tristan P.; Barber, Stuart; Williamson, Gary

Abstract

There is a considerable need to rationalize the membrane permeability and mechanism of transport for potential nutraceuticals. The aim of this investigation was to develop a theoretical permeability equation, based on a reported descriptive absorption model, enabling calculation of the transcellular component of absorption across Caco-2 monolayers. Published data for Caco-2 permeability of 30 drugs transported by the transcellular route were correlated with the descriptors 1-octanol/water distribution coefficient (log D, pH 7.4) and size, based on molecular mass. Nonlinear regression analysis was used to derive a set of model parameters a?, ??, and b? with an integrated molecular mass function. The new theoretical transcellular permeability (TTP) model obtained a good fit of the published data (R2 = 0.93) and predicted reasonably well (R2 = 0.86) the experimental apparent permeability coefficient (Papp) for nine non-training set compounds reportedly transported by the transcellular route. For the first time, the TTP model was used to predict the absorption characteristics of six phenolic acids, and this original investigation was supported by in vitro Caco-2 cell mechanistic studies, which suggested that deviation of the Papp value from the predicted transcellular permeability (Papptrans) may be attributed to involvement of active uptake, efflux transporters, or paracellular flux.

Citation

Farrell, T. L., Poquet, L., Dew, T. P., Barber, S., & Williamson, G. (2012). Predicting Phenolic Acid Absorption in Caco-2 Cells: A Theoretical Permeability Model and Mechanistic Study. Drug Metabolism and Disposition, 40(2), 397-406. https://doi.org/10.1124/dmd.111.041665

• Journal Article Type: Article
• Acceptance Date: Nov 17, 2011
• Online Publication Date: Nov 17, 2011
• Publication Date: Feb 1, 2012
• Deposit Date: Aug 3, 2020
• Journal: Drug Metabolism and Disposition
• Print ISSN: 0090-9556
• Electronic ISSN: 1521-009X
• Publisher: American Society for Pharmacology and Experimental Therapeutics
• Peer Reviewed: Peer Reviewed
• Volume: 40
• Issue: 2
• Pages: 397-406
• DOI: https://doi.org/10.1124/dmd.111.041665
• Public URL: https://nottingham-repository.worktribe.com/output/4678244
• Publisher URL: http://dmd.aspetjournals.org/content/40/2/397