DNA replication roadblocks caused by Cascade interference complexes are alleviated by RecG DNA repair helicase

Howard, Jamieson L.; Killelea, Tom; Hawkins, Michelle; Howard, Jamieson; McGlynn, Peter; Bolt, Edward L.

doi:10.1080/15476286.2018.1496773

All Outputs (4)

Cas1-Cas2 physically and functionally interacts with DnaK to modulate CRISPR Adaptation (2023)
Journal Article

Prokaryotic Cas1-Cas2 protein complexes generate adaptive immunity to mobile genetic elements (MGEs), by capture and integration of MGE DNA in to CRISPR sites. De novo immunity relies on naive adaptation-Cas1-Cas2 targeting of MGE DNA without the aid... Read More about Cas1-Cas2 physically and functionally interacts with DnaK to modulate CRISPR Adaptation.

A Tryptophan ‘Gate’ in the CRISPR-Cas3 Nuclease Controls ssDNA Entry into the Nuclease Site, That When Removed Results in Nuclease Hyperactivity (2021)
Journal Article

Cas3 is a ssDNA-targeting nuclease-helicase essential for class 1 prokaryotic CRISPR immunity systems, which has been utilized for genome editing in human cells. Cas3-DNA crystal structures show that ssDNA follows a pathway from helicase domains into... Read More about A Tryptophan ‘Gate’ in the CRISPR-Cas3 Nuclease Controls ssDNA Entry into the Nuclease Site, That When Removed Results in Nuclease Hyperactivity.

CRISPR-Cas adaptation in Escherichia coli requires RecBCD helicase but not nuclease activity, is independent of homologous recombination, and is antagonized by 5' ssDNA exonucleases (2018)
Journal Article

© The Author(s) 2018. Prokaryotic adaptive immunity is established against mobile genetic elements (MGEs) by 'naïve adaptation' when DNA fragments from a newly encountered MGE are integrated into CRISPR-Cas systems. In Escherichia coli, DNA integrati... Read More about CRISPR-Cas adaptation in Escherichia coli requires RecBCD helicase but not nuclease activity, is independent of homologous recombination, and is antagonized by 5' ssDNA exonucleases.

DNA replication roadblocks caused by Cascade interference complexes are alleviated by RecG DNA repair helicase (2018)
Journal Article

Cascade complexes underpin E. coli CRISPR-Cas immunity systems by stimulating "adaptation" reactions that update immunity and by initiating "interference" reactions that destroy invader DNA. Recognition of invader DNA in Cascade catalysed R-loops pro... Read More about DNA replication roadblocks caused by Cascade interference complexes are alleviated by RecG DNA repair helicase.