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All Outputs (57)

Production of membrane proteins for characterisation of their pheromone-sensing and antimicrobial resistance functions (2018)
Journal Article
Azam, A. A., Kinder, J. M., Khan, G. N., Alase, A., Ma, P., Liu, Y., …Phillips-Jones, M. K. (2018). Production of membrane proteins for characterisation of their pheromone-sensing and antimicrobial resistance functions. European Biophysics Journal, https://doi.org/10.1007/s00249-018-1325-z

Despite the importance of membrane proteins in cellular processes, studies of these hydrophobic proteins present major technical challenges, including expression and purification for structural and biophysical studies. A modified strategy of that pro... Read More about Production of membrane proteins for characterisation of their pheromone-sensing and antimicrobial resistance functions.

The Svedberg Lecture 2017. From nano to micro: the huge dynamic range of the analytical ultracentrifuge for characterizing the sizes, shapes and interactions of molecules and assemblies in biochemistry and polymer science (2018)
Journal Article
Harding, S. E., Wakefield, J., Gillis, R., Adams, G., & McQueen, C. (2018). The Svedberg Lecture 2017. From nano to micro: the huge dynamic range of the analytical ultracentrifuge for characterizing the sizes, shapes and interactions of molecules and assemblies in biochemistry and polymer science. European Biophysics Journal, 47(7), 697–707. https://doi.org/10.1007/s00249-018-1290-6

The analytical ultracentrifuge (AUC) invented by T. Svedberg has now become an extremely versatile and diverse tool in Biochemistry and Polymer Science for the characterisation of the sizes, shapes and interactions of particles ranging in size from a... Read More about The Svedberg Lecture 2017. From nano to micro: the huge dynamic range of the analytical ultracentrifuge for characterizing the sizes, shapes and interactions of molecules and assemblies in biochemistry and polymer science.

Characterisation of insulin analogues therapeutically available to patients (2018)
Journal Article
Adams, G. G., Meal, A., Morgan, P. S., Alzahrani, Q. E., Zobel, H., Lithgo, R., Kok, M. S., Besong, D. T., Jiwani, S. I., Ballance, S., Harding, S. E., Gillis, R. B., & Chayen, N. (2018). Characterisation of insulin analogues therapeutically available to patients. PLoS ONE, 13(3), Article e0195010. https://doi.org/10.1371/journal.pone.0195010

The structure and function of clinical dosage insulin and its analogues were assessed. This included ‘native insulins’ (human recombinant, bovine, porcine), ‘fast-acting analogues’ (aspart, glulisine, lispro) and ‘slow-acting analogues’ (glargine, de... Read More about Characterisation of insulin analogues therapeutically available to patients.

Controlled depolymerisation, as assessed by analytical ultracentrifugation, of low molecular weight chitosan for potential use in archaeological conservation (2018)
Journal Article
Wakefield, J. M., Gillis, R. B., Adams, G. G., McQueen, C., & Harding, S. E. (2018). Controlled depolymerisation, as assessed by analytical ultracentrifugation, of low molecular weight chitosan for potential use in archaeological conservation. European Biophysics Journal, 47(7), 769-775. https://doi.org/10.1007/s00249-018-1290-6

The heterogeneity and molecular weight of a chitosan of low molecular weight (molar mass) and low degree of acetylation (0.1), for potential use as a consolidant for decayed archaeological wood, has been examined by sedimentation velocity and sedimen... Read More about Controlled depolymerisation, as assessed by analytical ultracentrifugation, of low molecular weight chitosan for potential use in archaeological conservation.

Stabilisation of oil-in-water emulsions with non-chemical modified gelatinised starch (2018)
Journal Article
Kasprzak, M. M., Macnaughtan, W., Harding, S., Wilde, P., & Wolf, B. (2018). Stabilisation of oil-in-water emulsions with non-chemical modified gelatinised starch. Food Hydrocolloids, 81, https://doi.org/10.1016/j.foodhyd.2018.03.002

In this research, stabilisation of oil-in-water emulsions with non-chemically modified gelatinised starch is presented. Thus far only octenyl succinic anhydride(OSA) modified gelatinised starch has been known to adsorb at emulsion droplet interfaces,... Read More about Stabilisation of oil-in-water emulsions with non-chemical modified gelatinised starch.

Full hydrodynamic reversibility of the weak dimerization of vancomycin and elucidation of its interaction with VanS monomers at clinical concentration (2017)
Journal Article
Phillips-Jones, M. K., Lithgo, R., Dinu, V., Gillis, R. B., Harding, J. E., Adams, G. G., & Harding, S. E. (2017). Full hydrodynamic reversibility of the weak dimerization of vancomycin and elucidation of its interaction with VanS monomers at clinical concentration. Scientific Reports, 7(1), 1-10. https://doi.org/10.1038/s41598-017-12620-z

The reversibility and strength of the previously established dimerization of the important glycopeptide antibiotic vancomycin in four different aqueous solvents (including a medically-used formulation) have been studied using short-column sedimentati... Read More about Full hydrodynamic reversibility of the weak dimerization of vancomycin and elucidation of its interaction with VanS monomers at clinical concentration.

In situ polymerisation of isoeugenol as a green consolidation method for waterlogged archaeological wood (2017)
Journal Article
McHale, E., Steindal, C. C., Kutzke, H., Benneche, T., & Harding, S. E. (2017). In situ polymerisation of isoeugenol as a green consolidation method for waterlogged archaeological wood. Scientific Reports, 7, Article 46481. https://doi.org/10.1038/srep46481

Waterlogged archaeological wood is often in need of consolidation prior to drying to prevent shrinkage and cracking of the object. There is a need for new greener materials (than for example polyethylene glycol) and methods for consolidation to be de... Read More about In situ polymerisation of isoeugenol as a green consolidation method for waterlogged archaeological wood.

Assessing sedimentation equilibrium profiles in analytical ultracentrifugation experiments on macromolecules: from simple average molecular weight analysis to molecular weight distribution and interaction analysis (2016)
Journal Article
Harding, S. E., Gillis, R. B., & Adams, G. G. (in press). Assessing sedimentation equilibrium profiles in analytical ultracentrifugation experiments on macromolecules: from simple average molecular weight analysis to molecular weight distribution and interaction analysis. Biophysical Reviews, 8(4), https://doi.org/10.1007/s12551-016-0232-8

Molecular weights (molar masses), molecular weight distributions, dissociation constants and other interaction parameters are fundamental characteristics of proteins, nucleic acids, polysaccharides and glycoconjugates in solution. Sedimentation equil... Read More about Assessing sedimentation equilibrium profiles in analytical ultracentrifugation experiments on macromolecules: from simple average molecular weight analysis to molecular weight distribution and interaction analysis.

Foreword to ‘Quantitative and analytical relations in biochemistry’—a special issue in honour of Donald J. Winzor’s 80th birthday (2016)
Journal Article
Hall, D., & Harding, S. E. (2016). Foreword to ‘Quantitative and analytical relations in biochemistry’—a special issue in honour of Donald J. Winzor’s 80th birthday. Biophysical Reviews, 8(4), 269-277. https://doi.org/10.1007/s12551-016-0227-5

The purpose of this special issue is to honour Professor Donald J. Winzor’s long career as a researcher and scientific mentor, and to celebrate the milestone of his 80th birthday. Throughout his career, Don has been renowned for his development of cl... Read More about Foreword to ‘Quantitative and analytical relations in biochemistry’—a special issue in honour of Donald J. Winzor’s 80th birthday.

Solution conformation and flexibility of capsular polysaccharides from Neisseria meningitidis and glycoconjugates with the tetanus toxoid protein (2016)
Journal Article
Abdelhameed, A. S., Morris, G. A., Almutairi, F., Adams, G. G., Duvivier, P., Conrath, K., & Harding, S. E. (in press). Solution conformation and flexibility of capsular polysaccharides from Neisseria meningitidis and glycoconjugates with the tetanus toxoid protein. Scientific Reports, 6(1), 1-11. https://doi.org/10.1038/srep35588

The structural integrity of meningococcal native, micro-fluidized and activated capsular polysaccharides and their glycoconjugates – in the form most relevant to their potential use as vaccines (dilute solution) - have been investigated with respect... Read More about Solution conformation and flexibility of capsular polysaccharides from Neisseria meningitidis and glycoconjugates with the tetanus toxoid protein.

Both reversible self-association and structural changes underpin molecular viscoelasticity of mAb solutions (2016)
Journal Article
Sarangapani, P. S., Weaver, J., Parupudi, A., Besong, T. M., Adams, G. G., Harding, S. E., …Pathak, J. A. (2016). Both reversible self-association and structural changes underpin molecular viscoelasticity of mAb solutions. Journal of Pharmaceutical Sciences, 105(12), https://doi.org/10.1016/j.xphs.2016.08.020

The role of antibody structure (conformation) in solution rheology is probed. It is demonstrated here that pH-dependent changes in the tertiary structure of 2 mAb solutions lead to viscoelasticity and not merely a shear viscosity (η) increase. Steady... Read More about Both reversible self-association and structural changes underpin molecular viscoelasticity of mAb solutions.

Genetic improvement of tomato by targeted control of fruit softening (2016)
Journal Article
Uluisik, S., Chapman, N. H., Smith, R., Poole, M., Adams, G., Gillis, R. B., …Seymour, G. B. (2016). Genetic improvement of tomato by targeted control of fruit softening. Nature Biotechnology, 34, 950-952. https://doi.org/10.1038/nbt.3602

Controlling the rate of softening to extend shelf life was a key target for researchers engineering genetically modified (GM) tomatoes in the 1990s, but only modest improvements were achieved. Hybrids grown nowadays contain 'non-ripening mutations' t... Read More about Genetic improvement of tomato by targeted control of fruit softening.

The chelation of colonic luminal iron by a unique sodium alginate for the improvement of gastrointestinal health (2016)
Journal Article
Horniblow, R. D., Latunde-Dada, G. O., Harding, S. E., Schneider, M., Almutairi, F. M., Sahni, M., …Tselepis, C. (2016). The chelation of colonic luminal iron by a unique sodium alginate for the improvement of gastrointestinal health. Molecular Nutrition and Food Research, 60(9), 2098-2108. https://doi.org/10.1002/mnfr.201500882

Scope

Iron is an essential nutrient. However, in animal models, excess unabsorbed dietary iron residing within the colonic lumen has been shown to exacerbate inflammatory bowel disease and intestinal cancer. Therefore, the aims of this study were... Read More about The chelation of colonic luminal iron by a unique sodium alginate for the improvement of gastrointestinal health.

Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions (2016)
Journal Article
Hussain, R., Harding, S. E., Hughes, C. S., Ma, P., Patching, S. G., Edara, S., …Phillips-Jones, M. K. (2016). Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions. Biochemical Society Transactions, 44(3), 810-823. https://doi.org/10.1042/BST20160023

This article reviews current methods for the reliable heterologous overexpression in Escherichia coli and purification of milligram quantities of bacterial membrane sensor kinase (MSK) proteins belonging to the two-component signal transduction famil... Read More about Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions.

Molecular weight analysis of starches: which technique? (2016)
Journal Article
Harding, S. E., Adams, G. G., & Gillis, R. B. (2016). Molecular weight analysis of starches: which technique?. Starch/Stärke, 68(9-10), https://doi.org/10.1002/star.201600042

A number of methods are available to researchers for estimating molecular weights (molar masses) and molecular weight distributions of starches − or those in solution, using an appropriate solvent/solubilisation protocol. We outline the methods avail... Read More about Molecular weight analysis of starches: which technique?.

Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides (2016)
Book Chapter
Harding, S. E., Adams, G. G., Gillis, R. B., Almutairi, F. M., & Morris, G. A. (2016). Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides. In S. Uchiyama, F. Arisaka, W. F. Stafford, & T. M. Laue (Eds.), Analytical ultracentrifugation: instrumentation, software and applications (375-386). Springer. https://doi.org/10.1007/978-4-431-55985-6_18

Molar mass or ‘molecular weight’ is one of the most fundamental parameters describing a macromolecule. Because of their polydisperse nature, polysaccharides are usually described by distributions of molar mass. SEC-MALS (size exclusion chromatography... Read More about Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides.

A glycoconjugate of Haemophilus influenzae Type b capsular polysaccharide with tetanus toxoid protein: hydrodynamic properties mainly influenced by the carbohydrate (2016)
Journal Article
Abdelhameed, A. S., Adams, G. G., Morris, G. A., Almutairi, F. M., Duvivier, P., Conrath, K., & Harding, S. E. (2016). A glycoconjugate of Haemophilus influenzae Type b capsular polysaccharide with tetanus toxoid protein: hydrodynamic properties mainly influenced by the carbohydrate. Scientific Reports, 6(1), Article 22208. https://doi.org/10.1038/srep22208

Three important physical properties which may affect the performance of glycoconjugate vaccines against serious disease are molar mass (molecular weight), heterogeneity (polydispersity), and conformational flexibility in solution. The dilute solution... Read More about A glycoconjugate of Haemophilus influenzae Type b capsular polysaccharide with tetanus toxoid protein: hydrodynamic properties mainly influenced by the carbohydrate.

Coiled coil type neoglycoproteins presenting three lactose residues (2016)
Journal Article
Sweeney, S. M., Bullen, G. A., Gillis, R. B., Adams, G. G., Rowe, A. J., Harding, S. E., …Murphy, P. V. (2016). Coiled coil type neoglycoproteins presenting three lactose residues. Tetrahedron Letters, 57(13), https://doi.org/10.1016/j.tetlet.2016.02.005

Scaffold design, synthesis and application are relevant for biomedical research. For example, multivalent interactions, such as those between cell surface glycoproteins and lectins can influence the potency and duration of signalling. The spacing bet... Read More about Coiled coil type neoglycoproteins presenting three lactose residues.