Mary K. Phillips-Jones
Full hydrodynamic reversibility of the weak dimerization of vancomycin and elucidation of its interaction with VanS monomers at clinical concentration
Phillips-Jones, Mary K.; Lithgo, Ryan; Dinu, Vlad; Gillis, Richard B.; Harding, John E.; Adams, Gary G.; Harding, Stephen E.
Authors
Ryan Lithgo
Vlad Dinu
Richard B. Gillis
John E. Harding
Dr GARY ADAMS gary.adams@nottingham.ac.uk
Associate Professor
STEPHEN HARDING STEVE.HARDING@NOTTINGHAM.AC.UK
Professor of Applied Biochemistry
Abstract
The reversibility and strength of the previously established dimerization of the important glycopeptide antibiotic vancomycin in four different aqueous solvents (including a medically-used formulation) have been studied using short-column sedimentation equilibrium in the analytical ultracentrifuge and model-independent SEDFIT-MSTAR analysis across a range of loading concentrations. The change in the weight average molar mass Mw with loading concentration was consistent with a monomer-dimer equilibrium. Overlap of data sets of point weight average molar masses Mw(r) versus local concentration c(r) for different loading concentrations demonstrated a completely reversible equilibrium process. At the clinical infusion concentration of 5 mg.mL?1 all glycopeptide is dimerized whilst at 19 ?g.mL?1 (a clinical target trough serum concentration), vancomycin was mainly monomeric (
Citation
Phillips-Jones, M. K., Lithgo, R., Dinu, V., Gillis, R. B., Harding, J. E., Adams, G. G., & Harding, S. E. (2017). Full hydrodynamic reversibility of the weak dimerization of vancomycin and elucidation of its interaction with VanS monomers at clinical concentration. Scientific Reports, 7(1), 1-10. https://doi.org/10.1038/s41598-017-12620-z
Journal Article Type | Article |
---|---|
Acceptance Date | Aug 29, 2017 |
Online Publication Date | Oct 5, 2017 |
Publication Date | 2017-12 |
Deposit Date | Oct 11, 2017 |
Publicly Available Date | Oct 11, 2017 |
Journal | Scientific Reports |
Electronic ISSN | 2045-2322 |
Publisher | Nature Publishing Group |
Peer Reviewed | Peer Reviewed |
Volume | 7 |
Issue | 1 |
Article Number | 12697 |
Pages | 1-10 |
DOI | https://doi.org/10.1038/s41598-017-12620-z |
Keywords | Biopolymers in vivo, Physical chemistry |
Public URL | https://nottingham-repository.worktribe.com/output/886309 |
Publisher URL | https://www.nature.com/articles/s41598-017-12620-z |
Files
s41598-017-12620-z.pdf
(3 Mb)
PDF
Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
You might also like
Probing the effect of aroma compounds on the hydrodynamic properties of mucin glycoproteins
(2020)
Journal Article
Food biotechnology
(2020)
Journal Article
Downloadable Citations
About Repository@Nottingham
Administrator e-mail: discovery-access-systems@nottingham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search