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Full hydrodynamic reversibility of the weak dimerization of vancomycin and elucidation of its interaction with VanS monomers at clinical concentration

Phillips-Jones, Mary K.; Lithgo, Ryan; Dinu, Vlad; Gillis, Richard B.; Harding, John E.; Adams, Gary G.; Harding, Stephen E.

Authors

Dr MARY PHILLIPS-JONES Mary.Phillips-Jones@nottingham.ac.uk
Associate Professor in Polymer & Microbial Biophysics

Ryan Lithgo

Vlad Dinu

Richard B. Gillis

John E. Harding

Gary G. Adams

Stephen E. Harding



Abstract

The reversibility and strength of the previously established dimerization of the important glycopeptide antibiotic vancomycin in four different aqueous solvents (including a medically-used formulation) have been studied using short-column sedimentation equilibrium in the analytical ultracentrifuge and model-independent SEDFIT-MSTAR analysis across a range of loading concentrations. The change in the weight average molar mass Mw with loading concentration was consistent with a monomer-dimer equilibrium. Overlap of data sets of point weight average molar masses Mw(r) versus local concentration c(r) for different loading concentrations demonstrated a completely reversible equilibrium process. At the clinical infusion concentration of 5 mg.mL−1 all glycopeptide is dimerized whilst at 19 μg.mL−1 (a clinical target trough serum concentration), vancomycin was mainly monomeric (

Journal Article Type Article
Publication Date 2017-12
Journal Scientific Reports
Print ISSN 2045-2322
Electronic ISSN 2045-2322
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 7
Issue 1
Article Number 12697
Pages 1-10
APA6 Citation Phillips-Jones, M. K., Lithgo, R., Dinu, V., Gillis, R. B., Harding, J. E., Adams, G. G., & Harding, S. E. (2017). Full hydrodynamic reversibility of the weak dimerization of vancomycin and elucidation of its interaction with VanS monomers at clinical concentration. Scientific Reports, 7(1), 1-10. https://doi.org/10.1038/s41598-017-12620-z
DOI https://doi.org/10.1038/s41598-017-12620-z
Keywords Biopolymers in vivo, Physical chemistry
Publisher URL https://www.nature.com/articles/s41598-017-12620-z
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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