Real-time analysis of the binding of fluorescent VEGF165a to VEGFR2 in living cells: Effect of receptor tyrosine kinase inhibitors and fate of internalized agonist-receptor complexes

Kilpatrick, Laura E.; Friedman-Ohana, Rachel; Alcobia, Diana C.; Riching, Kristin; Peach, Chloe J.; Wheal, Amanda J.; Briddon, Stephen J.; Robers, Matthew B.; Zimmerman, Kris; Machleidt, Thomas; Wood, Keith V.; Woolard, Jeanette; Hill, Stephen J.

doi:10.1016/j.bcp.2017.04.006

All Outputs (2)

A non-imaging high throughput approach to chemical library screening at the unmodified adenosine-A3 receptor in living cells (2017)
Journal Article

Recent advances in fluorescent ligand technology have enabled the study of G protein-coupled receptors in their native environment without the need for genetic modification such as addition of N-terminal fluorescent or bioluminescent tags. Here, we h... Read More about A non-imaging high throughput approach to chemical library screening at the unmodified adenosine-A3 receptor in living cells.

Real-time analysis of the binding of fluorescent VEGF165a to VEGFR2 in living cells: Effect of receptor tyrosine kinase inhibitors and fate of internalized agonist-receptor complexes (2017)
Journal Article

© 2017 The Authors Vascular endothelial growth factor (VEGF) is an important mediator of angiogenesis. Here we have used a novel stoichiometric protein-labeling method to generate a fluorescent variant of VEGF (VEGF165a-TMR) labeled on a single cyste... Read More about Real-time analysis of the binding of fluorescent VEGF165a to VEGFR2 in living cells: Effect of receptor tyrosine kinase inhibitors and fate of internalized agonist-receptor complexes.