Research Repository

See what's under the surface


Human SETMAR is a DNA sequence-specific histone-methylase with a broad effect on the transcriptome (2018)
Journal Article
Tellier, M., & Chalmers, R. (2018). Human SETMAR is a DNA sequence-specific histone-methylase with a broad effect on the transcriptome. Nucleic Acids Research, doi:10.1093/nar/gky937

Transposons impart dynamism to the genomes they inhabit and their movements frequently rewire the control of nearby genes. Occasionally, their proteins are domesticated when they evolve a new function. SETMAR is a protein methylase with a sequence-sp... Read More

Transposase subunit architecture and its relationship to genome size and the rate of transposition in prokaryotes and eukaryotes (2018)
Journal Article
Blundell-Hunter, G., Tellier, M., & Chalmers, R. (2018). Transposase subunit architecture and its relationship to genome size and the rate of transposition in prokaryotes and eukaryotes. Nucleic Acids Research, doi:10.1093/nar/gky794. ISSN 0305-1048

Cut-and-paste transposons are important tools for mutagenesis, gene-delivery and DNA sequencing applications. At the molecular level, the most thoroughly understood are Tn5 and Tn10 in bacteria, and mariner and hAT elements in eukaryotes. All bacteri... Read More

A single active site in the mariner transposase cleaves DNA strands of opposite polarity (2017)
Journal Article
Claeys Bouuaert, C., & Chalmers, R. (in press). A single active site in the mariner transposase cleaves DNA strands of opposite polarity. Nucleic Acids Research, gkx826, doi:10.1093/nar/gkx826

The RNase H structural fold defines a large family of nucleic acid metabolizing enzymes that catalyze phosphoryl transfer reactions using two divalent metal ions in the active site. Almost all of these reactions involve only one strand of the nucleic... Read More

Crosstalk between transposase subunits during cleavage of the mariner transposon (2014)
Journal Article
Claeys Bouuaert, C., Walker, N., Liu, D., & Chalmers, R. (2014). Crosstalk between transposase subunits during cleavage of the mariner transposon. Nucleic Acids Research, 42(9), doi:10.1093/nar/gku172. ISSN 0305-1048

Mariner transposition is a complex reaction that involves three recombination sites and six strand breaking and joining reactions. This requires precise spatial and temporal coordination between the different components to ensure a productive outcome... Read More

Hyperactive mariner transposons are created by mutations that disrupt allosterism and increase the rate of transposon end synapsis (2013)
Journal Article
Liu, D., & Chalmers, R. (2014). Hyperactive mariner transposons are created by mutations that disrupt allosterism and increase the rate of transposon end synapsis. Nucleic Acids Research, 42(4), doi:10.1093/nar/gkt1218. ISSN 0305-1048

New applications for transposons in vertebrate genetics have spurred efforts to develop hyperactive variants. Typically, a genetic screen is used to identify several hyperactive point mutations, which are then incorporated in a single transposase gen... Read More

Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome (2013)
Journal Article
for the integration of DNA into the human genome. BMC Biotechnology, 13, doi:10.1186/1472-6750-13-87. ISSN 1472-6750

Background: Phage-encoded serine integrases, such as φC31 integrase, are widely used for genome engineering. Fifteen such integrases have been described but their utility for genome engineering has not been compared in uniform assays. Results: We h... Read More

Identification of multiple binding sites for the THAP domain of the Galileo transposase in the long terminal inverted-repeats (2013)
Journal Article
Marzo, M., Liu, D., Ruiz, A., & Chalmers, R. (2013). Identification of multiple binding sites for the THAP domain of the Galileo transposase in the long terminal inverted-repeats. Gene, 525(1), doi:10.1016/j.gene.2013.04.050. ISSN 0378-1119

Galileo is a DNA transposon responsible for the generation of several chromosomal inversions in Drosophila. In contrast to other members of the P-element superfamily, it has unusually long terminal inverted-repeats (TIRs) that resemble those of Foldb... Read More

H-NS mediates the dissociation of a refractory protein–DNA complex during Tn10/IS10 transposition (2011)
Journal Article
Liu, D., Haniford, D. B., & Chalmers, R. (2011). H-NS mediates the dissociation of a refractory protein–DNA complex during Tn10/IS10 transposition. Nucleic Acids Research, 39(15), doi:10.1093/nar/gkr309. ISSN 0305-1048

Tn10/IS10 transposition takes place in the context of a protein–DNA complex called a transpososome. During the reaction, the transpososome undergoes several conformational changes. The host proteins IHF and H-NS, which also are global regulators of g... Read More

Base flipping in Tn10 transposition: an active flip and capture mechanism (2009)
Journal Article
Bischerour, J., & Chalmers, R. (2009). Base flipping in Tn10 transposition: an active flip and capture mechanism. PLoS ONE, 4(7), doi:10.1371/journal.pone.0006201. ISSN 1932-6203

The bacterial Tn5 and Tn10 transposases have a single active site that cuts both strands of DNA at their respective transposon ends. This is achieved using a hairpin intermediate that requires the DNA to change conformation during the reaction. In Tn... Read More