All Outputs (22)

Global co-regulatory cross talk between m6A and m5C RNA methylation systems coordinate cellular responses and brain disease pathways (2024)
Journal Article
Chukwuma Orji, O., Stones, J., Bagia, S., Markus, R., Demirbugen Öz, M., & Knight, H. (in press). Global co-regulatory cross talk between m6A and m5C RNA methylation systems coordinate cellular responses and brain disease pathways. Molecular Neurobiology,

N6 adenosine and C5 cytosine modification of mRNAs, tRNAs and rRNAs are regulated by the behaviour of distinct sets of writer, reader and eraser effector proteins which are conventionally considered to function independently. Here, we provide evidenc... Read More about Global co-regulatory cross talk between m6A and m5C RNA methylation systems coordinate cellular responses and brain disease pathways.

Plasmodium NEK1 coordinates MTOC organisation and kinetochore attachment during rapid mitosis in male gamete formation (2024)
Journal Article
Zeeshan, M., Rashpa, R., Ferguson, D. J., Mckeown, G., Nugmanova, R., Subudhi, A. K., Beyeler, R., Pashley, S. L., Markus, R., Brady, D., Roques, M., Bottrill, A. R., Fry, A. M., Pain, A., Vaughan, S., Holder, A. A., Tromer, E. C., Brochet, M., & Tewari, R. (2024). Plasmodium NEK1 coordinates MTOC organisation and kinetochore attachment during rapid mitosis in male gamete formation. PLoS Biology, 22(9), Article e3002802. https://doi.org/10.1371/journal.pbio.3002802

Mitosis is an important process in the cell cycle required for cells to divide. Never in mitosis (NIMA)-like kinases (NEKs) are regulators of mitotic functions in diverse organisms. Plasmodium spp., the causative agent of malaria is a divergent unice... Read More about Plasmodium NEK1 coordinates MTOC organisation and kinetochore attachment during rapid mitosis in male gamete formation.

m6A mRNA methylation in human brain is disrupted in Lewy body disorders (2023)
Journal Article
Martinez De La Cruz, B., Gell, C., Markus, R., Macdonald, I., Fray, R., & Knight, H. M. (2023). m6A mRNA methylation in human brain is disrupted in Lewy body disorders. Neuropathology and Applied Neurobiology, 49(1), Article e12885. https://doi.org/10.1111/nan.12885

N6-methyladenosine modification of RNA (m6A) regulates translational control which may influence neuronal dysfunction underlying neurodegenerative diseases. Using microscopy and a machine learning approach, we performed cellular profiling of m6A-RNA... Read More about m6A mRNA methylation in human brain is disrupted in Lewy body disorders.

Plasmodium SAS4: basal body component of male cell which is dispensable for parasite transmission (2022)
Journal Article
Zeeshan, M., Brady, D., Markus, R., Vaughan, S., Ferguson, D., Holder, A. A., & Tewari, R. (2022). Plasmodium SAS4: basal body component of male cell which is dispensable for parasite transmission. Life Science Alliance, 5(9), Article e202101329. https://doi.org/10.26508/lsa.202101329

The centriole/basal body (CBB) is an evolutionarily conserved organelle acting as a microtubule organising centre (MTOC) to nucleate cilia, flagella, and the centrosome. SAS4/CPAP is a conserved component associated with BB biogenesis in many model f... Read More about Plasmodium SAS4: basal body component of male cell which is dispensable for parasite transmission.

Modifying the m6A brain methylome by ALKBH5-mediated demethylation: a new contender for synaptic tagging (2021)
Journal Article
Martinez De La Cruz, B., Markus, R., Malla, S., Haig, M. I., Gell, C., Sang, F., …Knight, H. M. (2021). Modifying the m6A brain methylome by ALKBH5-mediated demethylation: a new contender for synaptic tagging. Molecular Psychiatry, 26(12), 7141-7153. https://doi.org/10.1038/s41380-021-01282-z

Synaptic plasticity processes, which underlie learning and memory formation, require RNA to be translated local to synapses. The synaptic tagging hypothesis has previously been proposed to explain how mRNAs are available at specific activated synapse... Read More about Modifying the m6A brain methylome by ALKBH5-mediated demethylation: a new contender for synaptic tagging.

Light-controlled micron-scale molecular motion (2021)
Journal Article
Samperi, M., Bdiri, B., Sleet, C. D., Markus, R., Mallia, A. R., Pérez-García, L., & Amabilino, D. B. (2021). Light-controlled micron-scale molecular motion. Nature Chemistry, 13(12), 1200-1206. https://doi.org/10.1038/s41557-021-00791-2

The micron-scale movement of biomolecules along supramolecular pathways, mastered by nature, is a remarkable system requiring strong yet reversible interactions between components under the action of a suitable stimulus. Responsive microscopic system... Read More about Light-controlled micron-scale molecular motion.

Real-time dynamics of Plasmodium NDC80 reveals unusual modes of chromosome segregation during parasite proliferation (2020)
Journal Article
Zeeshan, M., Pandey, R., Ferguson, D. J. P., Tromer, E. C., Markus, R., Abel, S., …Tewari, R. (2020). Real-time dynamics of Plasmodium NDC80 reveals unusual modes of chromosome segregation during parasite proliferation. Journal of Cell Science, 134(5), Article jcs.245753. https://doi.org/10.1242/jcs.245753

Eukaryotic cell proliferation requires chromosome replication and precise segregation to ensure daughter cells have identical genomic copies. The genus Plasmodium, the causative agent of malaria, displays remarkable aspects of nuclear division throug... Read More about Real-time dynamics of Plasmodium NDC80 reveals unusual modes of chromosome segregation during parasite proliferation.

Cell type–specific super-resolution imaging reveals an increase in calcium-permeable AMPA receptors at spinal peptidergic terminals as an anatomical correlate of inflammatory pain (2019)
Journal Article
Woodhams, S. G., Markus, R., Gowler, P. R., Self, T. J., & Chapman, V. (2019). Cell type–specific super-resolution imaging reveals an increase in calcium-permeable AMPA receptors at spinal peptidergic terminals as an anatomical correlate of inflammatory pain. PAIN, 160(11), 2641-2650. https://doi.org/10.1097/j.pain.0000000000001672

Spinal hyperexcitability is a key event in the development of persistent pain, and arises partly from alterations in the number and localization of α-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA)-type glutamate receptors. However, determinin... Read More about Cell type–specific super-resolution imaging reveals an increase in calcium-permeable AMPA receptors at spinal peptidergic terminals as an anatomical correlate of inflammatory pain.

Mechanistic investigations into the encapsulation and release of small molecules and proteins from a supramolecular nucleoside gel in vitro and in vivo (2019)
Journal Article
Faidra Angelerou, M. G., Markus, R., Paraskevopoulou, V., Foralosso, R., Clarke, P., Alvarez, C. V., …Marlow, M. (2020). Mechanistic investigations into the encapsulation and release of small molecules and proteins from a supramolecular nucleoside gel in vitro and in vivo. Journal of Controlled Release, 317, 118-129. https://doi.org/10.1016/j.jconrel.2019.10.011

Supramolecular gels have recently emerged as promising biomaterials for the delivery of a wide range of bioactive molecules, from small hydrophobic drugs to large biomolecules such as proteins. Although it has been demonstrated that each encapsulated... Read More about Mechanistic investigations into the encapsulation and release of small molecules and proteins from a supramolecular nucleoside gel in vitro and in vivo.

Live Simultaneous Monitoring of Mineral Deposition and Lipid Accumulation in Differentiating Stem Cells (2019)
Journal Article
De Melo, N., McGinlay, S., Markus, R., Macri-Pellizzeri, L., Symonds, M. E., Ahmed, I., & Sottile, V. (2019). Live Simultaneous Monitoring of Mineral Deposition and Lipid Accumulation in Differentiating Stem Cells. Biomimetics, 4(3), 1-10. https://doi.org/10.3390/biomimetics4030048

Mesenchymal stem cells (MSCs) are progenitors for bone-forming osteoblasts and lipid-storing adipocytes, two major lineages co-existing in bone marrow. When isolated in vitro, these stem cells recapitulate osteoblast or adipocyte formation if treated... Read More about Live Simultaneous Monitoring of Mineral Deposition and Lipid Accumulation in Differentiating Stem Cells.

Mechanistic insight into heterogeneity of trans-plasma membrane electron transport in cancer cell types (2019)
Journal Article
Sherman, H. G., Jovanovic, C., Abuawad, A., Kim, D., Collins, H., Dixon, J. E., …Rawson, F. J. (2019). Mechanistic insight into heterogeneity of trans-plasma membrane electron transport in cancer cell types. BBA - Bioenergetics, 1860(8), 628-639. https://doi.org/10.1016/j.bbabio.2019.06.012

Trans-plasma membrane electron transfer (tMPET) is a process by which reducing equivalents, either electrons or reductants like ascorbic acid, are exported to the extracellular environment by the cell. TPMET is involved in a number of physiological p... Read More about Mechanistic insight into heterogeneity of trans-plasma membrane electron transport in cancer cell types.

Plasmodium centrin PbCEN-4 localizes to the putative MTOC and is dispensable for malaria parasite proliferation (2018)
Journal Article
Roques, M., Stanway, R. R., Rea, E. I., Markus, R., Brady, D., Holder, A. A., …Tewari, R. (2018). Plasmodium centrin PbCEN-4 localizes to the putative MTOC and is dispensable for malaria parasite proliferation. Biology Open, 8(1), Article bio.036822. https://doi.org/10.1242/bio.036822

Centrins are calmodulin-like phosphoproteins present in the centrosome and play an active role in the duplication, separation and organization of centrosomal structures such as the microtubule-organizing centre (MTOC) during mitosis. They are also ma... Read More about Plasmodium centrin PbCEN-4 localizes to the putative MTOC and is dispensable for malaria parasite proliferation.

Rapid and accurate analysis of stem cell-derived extracellular vesicles with super resolution microscopy and live imaging (2018)
Journal Article
Nizamudeen, Z., Markus, R., Lodge, R., Parmenter, C., Platt, M., Chakrabarti, L., & Sottile, V. (2018). Rapid and accurate analysis of stem cell-derived extracellular vesicles with super resolution microscopy and live imaging. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1865(12), 1891-1900. https://doi.org/10.1016/j.bbamcr.2018.09.008

Extracellular vesicles (EVs) have prevalent roles in cancer biology and regenerative medicine. Conventional techniques for characterising EVs including electron microscopy (EM), nanoparticle tracking analysis (NTA) and tuneable resistive pulse sensin... Read More about Rapid and accurate analysis of stem cell-derived extracellular vesicles with super resolution microscopy and live imaging.

Quantitative methods to monitor RNA biomarkers in myotonic dystrophy (2018)
Journal Article
Wojciechowska, M., Sobczak, K., Kozlowski, P., Sedehizadeh, S., Wojtkowiak-Szlachcic, A., Czubak, K., Markus, R., Lusakowska, A., Kaminska, A., & Brook, J. D. (in press). Quantitative methods to monitor RNA biomarkers in myotonic dystrophy. Scientific Reports, 8, Article 5885. https://doi.org/10.1038/s41598-018-24156-x

Myotonic dystrophy type 1 (DM1) and type 2 (DM2) are human neuromuscular disorders associated with mutations of simple repetitive sequences in afected genes. The abnormal expansion of CTG repeats in the 3′-UTR of the DMPK gene elicits DM1, whereas el... Read More about Quantitative methods to monitor RNA biomarkers in myotonic dystrophy.

Immunostaining for DNA modifications: computational analysis of confocal images (2017)
Journal Article
Ramsawhook, A., Lewis, L. C., Eleftheriou, M., Abakir, A., Durczak, P. M., Markus, R., …Ruzov, A. (2017). Immunostaining for DNA modifications: computational analysis of confocal images. Journal of Visualized Experiments, Article e56318. https://doi.org/10.3791/56318

For several decades, 5-methylcytosine (5mC) has been thought to be the only DNA modification with a functional significance in metazoans. The discovery of enzymatic oxidation of 5mC to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carb... Read More about Immunostaining for DNA modifications: computational analysis of confocal images.

Real-time measurement of the intracellular pH of yeast cells during glucose metabolism using ratiometric fluorescent nanosensors (2017)
Journal Article
Elsutohy, M. M., Chauhan, V. M., Markus, R., Kyyaly, M. A., Tendler, S. J., & Aylott, J. W. (2017). Real-time measurement of the intracellular pH of yeast cells during glucose metabolism using ratiometric fluorescent nanosensors. Nanoscale, 9(18), 5904-5911. https://doi.org/10.1039/c7nr00906b

Intracellular pH is a key parameter that influences many biochemical and metabolic pathways that can also be used as an indirect marker to monitor metabolic and intracellular processes. Herein, we utilise ratiometric fluorescent pH-sensitive nanosens... Read More about Real-time measurement of the intracellular pH of yeast cells during glucose metabolism using ratiometric fluorescent nanosensors.

The N-terminus of survivin is a mitochondrial-targeting sequence and Src regulator (2016)
Journal Article
Dunajova, L., Cash, E., Markus, R., Rochette, S., Townley, A. R., & Wheatley, S. P. (in press). The N-terminus of survivin is a mitochondrial-targeting sequence and Src regulator. Journal of Cell Science, 129, https://doi.org/10.1242/jcs.183277

Survivin is a cancer-associated protein that exists in several locations in the cell. Its cytoplasmic residence in interphase cells is governed by CRM1-mediated nuclear exportation, and its localisation during mitosis to the centromeres and midzone m... Read More about The N-terminus of survivin is a mitochondrial-targeting sequence and Src regulator.

Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824 (2016)
Journal Article
Willson, B. J., Kovács, K., Wilding-Steele, T., Markus, R., Winzer, K., & Minton, N. P. (2016). Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824. Biotechnology for Biofuels, 9(1), Article 109. https://doi.org/10.1186/s13068-016-0526-x

Background: The use of fossil fuels is no longer tenable. Not only are they a finite resource, their use is damaging the environment through pollution and global warming. Alternative, environmentally friendly, renewable sources of chemicals and fuels... Read More about Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824.

Analysis of 1-Hz GPS data for the estimation of long-period surface motion of Tohoku-Oki Mw9.0 2011 earthquake (2016)
Presentation / Conference Contribution
Psimoulis, P., Houlié, N., Clotaire, M., Meindl, M., & Rothacher, M. Analysis of 1-Hz GPS data for the estimation of long-period surface motion of Tohoku-Oki Mw9.0 2011 earthquake. Presented at 3rd Joint International Symposium on Deformation Monitoring

The GPS displacement time series are today mostly used for the estimation of the transient (period <2sec) and coseismic (static) displacement of an earthquake, while the estimation of the velocity and acceleration is based only seismic networks. In t...

Consistency of PPP GPS and strong-motion records: case study of Mw9.0 Tohoku-Oki 2011 earthquake (2015)
Journal Article
Psimoulis, P., Houlié, N., Meindl, M., & Rothacher, M. (2015). Consistency of PPP GPS and strong-motion records: case study of Mw9.0 Tohoku-Oki 2011 earthquake. Smart structures and systems, 16(2), https://doi.org/10.12989/sss.2015.16.2.347

GPS and strong-motion sensors are broadly used for the monitoring of structural health and Earth surface motions, focusing on response of structures, earthquake characterization and rupture modeling. Several studies have shown the consistency of the... Read More about Consistency of PPP GPS and strong-motion records: case study of Mw9.0 Tohoku-Oki 2011 earthquake.