Zubair Nizamudeen
Rapid and accurate analysis of stem cell-derived extracellular vesicles with super resolution microscopy and live imaging
Nizamudeen, Zubair; Markus, Robert; Lodge, Rhys; Parmenter, Christopher; Platt, Mark; Chakrabarti, Lisa; Sottile, Virginie
Authors
Robert Markus
Rhys Lodge
Dr CHRISTOPHER PARMENTER CHRISTOPHER.PARMENTER@NOTTINGHAM.AC.UK
SENIOR RESEARCH FELLOW
Mark Platt
Professor LISA CHAKRABARTI LISA.CHAKRABARTI@NOTTINGHAM.AC.UK
PROFESSOR OF MITOCHONDRIAL BIOLOGY
Virginie Sottile
Abstract
Extracellular vesicles (EVs) have prevalent roles in cancer biology and regenerative medicine. Conventional techniques for characterising EVs including electron microscopy (EM), nanoparticle tracking analysis (NTA) and tuneable resistive pulse sensing (TRPS), have been reported to produce high variability in particle count (EM) and poor sensitivity in detecting EVs below 50 nm in size (NTA and TRPS), making accurate and unbiased EV analysis technically challenging. This study introduces direct stochastic optical reconstruction microscopy (d-STORM) as an efficient and reliable characterisation approach for stem cell-derived EVs. Using a photo-switchable lipid dye, d-STORM imaging enabled rapid detection of EVs down to 20–30 nm in size with higher sensitivity and lower variability compared to EM, NTA and TRPS techniques. Imaging of EV uptake by live stem cells in culture further confirmed the potential of this approach for downstream cell biology applications and for the analysis of vesicle-based cell-cell communication.
Citation
Nizamudeen, Z., Markus, R., Lodge, R., Parmenter, C., Platt, M., Chakrabarti, L., & Sottile, V. (2018). Rapid and accurate analysis of stem cell-derived extracellular vesicles with super resolution microscopy and live imaging. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1865(12), 1891-1900. https://doi.org/10.1016/j.bbamcr.2018.09.008
Journal Article Type | Article |
---|---|
Acceptance Date | Sep 23, 2018 |
Online Publication Date | Oct 2, 2018 |
Publication Date | Dec 1, 2018 |
Deposit Date | Oct 3, 2018 |
Publicly Available Date | Oct 5, 2018 |
Journal | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research |
Print ISSN | 0167-4889 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 1865 |
Issue | 12 |
Pages | 1891-1900 |
DOI | https://doi.org/10.1016/j.bbamcr.2018.09.008 |
Keywords | Extracellular vesicle; Super-resolution microscopy; Stem cell |
Public URL | https://nottingham-repository.worktribe.com/output/1144467 |
Publisher URL | https://www.sciencedirect.com/science/article/pii/S0167488918304129 |
Contract Date | Oct 5, 2018 |
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Rapid and accurate analysis of stem cell-derived extracellular vesicles with super resolution microscopy and live imaging
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Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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