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All Outputs (31)

A single-cell atlas of pig gastrulation as a resource for comparative embryology (2024)
Journal Article
Simpson, L., Strange, A., Klisch, D., Kraunsoe, S., Azami, T., Goszczynski, D., …Alberio, R. (2024). A single-cell atlas of pig gastrulation as a resource for comparative embryology. Nature Communications, 15(1), Article 5210. https://doi.org/10.1038/s41467-024-49407-6

Cell-fate decisions during mammalian gastrulation are poorly understood outside of rodent embryos. The embryonic disc of pig embryos mirrors humans, making them a useful proxy for studying gastrulation. Here we present a single-cell transcriptomic at... Read More about A single-cell atlas of pig gastrulation as a resource for comparative embryology.

NANOG controls testicular germ cell tumour stemness through regulation of MIR9-2 (2024)
Journal Article
Cardenas, R. P., Zyoud, A., McIntyre, A., Alberio, R., Mongan, N. P., & Allegrucci, C. (2024). NANOG controls testicular germ cell tumour stemness through regulation of MIR9-2. Stem Cell Research and Therapy, 15(1), Article 128. https://doi.org/10.1186/s13287-024-03724-1

Background
Testicular germ cell tumours (TGCTs) represent a clinical challenge; they are most prevalent in young individuals and are triggered by molecular mechanisms that are not fully understood. The origin of TGCTs can be traced back to primordia... Read More about NANOG controls testicular germ cell tumour stemness through regulation of MIR9-2.

Interspecies control of development during mammalian gastrulation (2023)
Journal Article
Simpson, L., & Alberio, R. (2023). Interspecies control of development during mammalian gastrulation. Emerging Topics in Life Sciences, 7(4), 397-408. https://doi.org/10.1042/etls20230083

Gastrulation represents a pivotal phase of development and aberrations during this period can have major consequences, from minor anatomical deviations to severe congenital defects. Animal models are used to study gastrulation, however, there is cons... Read More about Interspecies control of development during mammalian gastrulation.

Understanding conceptus-maternal interactions: what tools do we need to develop? (2023)
Journal Article
Butt, Z., Tinning, H., O'Connell, M. J., Fenn, J., Alberio, R., & Forde, N. (2023). Understanding conceptus-maternal interactions: what tools do we need to develop?. Reproduction, Fertility and Development, 36(2), 81-92. https://doi.org/10.1071/RD23181

Communication between the maternal endometrium and developing embryo/conceptus is critical to support successful pregnancy to term. Studying the peri-implantation period of pregnancy is critical as this is when most pregnancy loss occurs in cattle. O... Read More about Understanding conceptus-maternal interactions: what tools do we need to develop?.

NANOG is required to establish the competence for germ-layer differentiation in the basal tetrapod axolotl (2023)
Journal Article
Simpson, L. A., Crowley, D., Forey, T., Acosta, H., Ferjentsik, Z., Chatfield, J., …Johnson, A. D. (2023). NANOG is required to establish the competence for germ-layer differentiation in the basal tetrapod axolotl. PLoS Biology, 21(6), Article e3002121. https://doi.org/10.1371/journal.pbio.3002121

Pluripotency defines the unlimited potential of individual cells of vertebrate embryos, from which all adult somatic cells and germ cells are derived. Understanding how the programming of pluripotency evolved has been obscured in part by a lack of da... Read More about NANOG is required to establish the competence for germ-layer differentiation in the basal tetrapod axolotl.

Review: A barnyard in the lab: prospect of generating animal germ cells for breeding and conservation (2023)
Journal Article
Strange, A., & Alberio, R. (2023). Review: A barnyard in the lab: prospect of generating animal germ cells for breeding and conservation. Animal, 17, Article 100753. https://doi.org/10.1016/j.animal.2023.100753

In vitro gametogenesis (IVG) offers broad opportunities for gaining detailed new mechanistic knowledge of germ cell biology that will enable progress in the understanding of human infertility, as well as for applications in the conservation of endang... Read More about Review: A barnyard in the lab: prospect of generating animal germ cells for breeding and conservation.

In vitro culture of ovine embryos up to early gastrulating stages (2022)
Journal Article
Ramos-Ibeas, P., González-Brusi, L., Used, M. T., Cocero, M. J., Marigorta, P., Alberio, R., & Bermejo-Álvarez, P. (2022). In vitro culture of ovine embryos up to early gastrulating stages. Development, 149(6), Article dev199743. https://doi.org/10.1242/dev.199743

Developmental failures occurring shortly after blastocyst hatching from the zona pellucida constitute a major cause of pregnancy losses in both humans and farm ungulates. The developmental events occurring following hatching in ungulates include the... Read More about In vitro culture of ovine embryos up to early gastrulating stages.

Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species (2021)
Journal Article
Kinoshita, M., Kobayashi, T., Planells, B., Klisch, D., Spindlow, D., Masaki, H., …Smith, A. (2021). Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species. Development, 148(23), Article dev199901. https://doi.org/10.1242/dev.199901

Despite four decades of effort, robust propagation of pluripotent stem cells from livestock animals remains challenging. The requirements for self-renewal are unclear and the relationship of cultured stem cells to pluripotent cells resident in the em... Read More about Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species.

Germline competent mesoderm: the substrate for vertebrate germline and somatic stem cells? (2021)
Journal Article
Savage, A. M., Alberio, R., & Johnson, A. D. (2021). Germline competent mesoderm: the substrate for vertebrate germline and somatic stem cells?. Biology Open, 10(10), Article bio058890. https://doi.org/10.1242/bio.058890

In vitro production of tissue-specific stem cells [e.g. haematopoietic stem cells (HSCs)] is a key goal of regenerative medicine. However, recent efforts to produce fully functional tissue-specific stem cells have fallen short. One possible cause of... Read More about Germline competent mesoderm: the substrate for vertebrate germline and somatic stem cells?.

Nuclear transfer and the development of genetically modified/gene edited livestock (2021)
Journal Article
Alberio, R., & Wolf, E. (2021). Nuclear transfer and the development of genetically modified/gene edited livestock. Reproduction, 162(1), F59-F68. https://doi.org/10.1530/REP-21-0078

The birth and adult development of 'Dolly' the sheep, the first mammal produced by the transfer of a terminally differentiated cell nucleus into an egg, provided unequivocal evidence of nuclear equivalence among somatic cells. This ground-breaking ex... Read More about Nuclear transfer and the development of genetically modified/gene edited livestock.

Nuclear transfer and the development of genetically modified/gene edited livestock (2021)
Journal Article
Alberio, R., & Wolf, E. (2021). Nuclear transfer and the development of genetically modified/gene edited livestock. Reproduction, 162(1), F59-F68. https://doi.org/10.1530/REP-21-0078

The birth and adult development of 'Dolly' the sheep, the first mammal produced by the transfer of a terminally differentiated cell nucleus into an egg, provided unequivocal evidence of nuclear equivalence among somatic cells. This ground-breaking ex... Read More about Nuclear transfer and the development of genetically modified/gene edited livestock.

Conserved features of non-primate bilaminar disc embryos and the germline (2021)
Journal Article
Alberio, R., Kobayashi, T., & Surani, M. A. (2021). Conserved features of non-primate bilaminar disc embryos and the germline. Stem Cell Reports, 16(5), 1078-1092. https://doi.org/10.1016/j.stemcr.2021.03.011

Post-implantation embryo development commences with a bilaminar disc in most mammals, including humans. Whereas access to early human embryos is limited and subject to greater ethical scrutiny, studies on non-primate embryos developing as bilaminar d... Read More about Conserved features of non-primate bilaminar disc embryos and the germline.

Specification and epigenomic resetting of the pig germline exhibit conservation with the human lineage (2021)
Journal Article
Zhu, Q., Sang, F., Withey, S., Tang, W., Dietmann, S., Klisch, D., …Alberio, R. (2021). Specification and epigenomic resetting of the pig germline exhibit conservation with the human lineage. Cell Reports, 34(6), Article 108735. https://doi.org/10.1016/j.celrep.2021.108735

Investigations of the human germline and programming are challenging because of limited access to embryonic material. However, the pig as a model may provide insights into transcriptional network and epigenetic reprogramming applicable to both specie... Read More about Specification and epigenomic resetting of the pig germline exhibit conservation with the human lineage.

Untangling early embryo development using single cell genomics (2020)
Journal Article
Alberio, R. (2020). Untangling early embryo development using single cell genomics. Theriogenology, 150, 55-58. https://doi.org/10.1016/j.theriogenology.2020.01.062

The zygote undergoes five cell divisions prior to the first signs of lineage segregation. Blastocyst formation requires segregation of the trophectoderm, needed for implantation, and the inner cell mass, which differentiate towards the major lineages... Read More about Untangling early embryo development using single cell genomics.

Regulation of Cell Fate Decisions in Early Mammalian Embryos (2019)
Journal Article
Alberio, R. (2020). Regulation of Cell Fate Decisions in Early Mammalian Embryos. Annual Review of Animal Biosciences, 8(1), 377-393. https://doi.org/10.1146/annurev-animal-021419-083841

Early embryogenesis is characterized by the segregation of cell lineages that fulfill critical roles in the establishment of pregnancy and development of the fetus. The formation of the blastocyst marks the emergence of extraembryonic precursors, nee... Read More about Regulation of Cell Fate Decisions in Early Mammalian Embryos.

A dose-dependent response to MEK inhibition determines hypoblast fate in bovine embryos (2019)
Journal Article
Canizo, J. R., Ynsaurralde Rivolta, A. E., Vazquez Echegaray, C., Suvá, M., Alberio, V., Aller, J. F., …Alberio, R. (2019). A dose-dependent response to MEK inhibition determines hypoblast fate in bovine embryos. BMC Developmental Biology, 19(1), 1-13. https://doi.org/10.1186/s12861-019-0193-9

Background
The segregation of the hypoblast and the emergence of the pluripotent epiblast mark the final stages of blastocyst formation in mammalian embryos. In bovine embryos the formation of the hypoblast has been partially studied, and evidence s... Read More about A dose-dependent response to MEK inhibition determines hypoblast fate in bovine embryos.

Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis (2019)
Journal Article
Ramos-Ibeas, P., Sang, F., Zhu, Q., Wang, W. W., Withey, S., Klisch, D., …Alberio, R. (2019). Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis. Nature Communications, 10, Article 500. https://doi.org/10.1038/s41467-019-08387-8

High-resolution molecular programmes delineating the cellular foundations of mammalian embryogenesis have emerged recently. Similar analysis of human embryos is limited to pre-implantation stages, since early post-implantation embryos are largely ina... Read More about Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis.

Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system (2018)
Journal Article
Canizo, J. R., Vazquez Echegaray, C., Klisch, D., Aller, J. F., Paz, D. A., Alberio, R. H., …Guberman, A. S. (2018). Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system. BMC Research Notes, 11, Article 509. https://doi.org/10.1186/s13104-018-3627-8

Objectives

The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this study, different strategies fo... Read More about Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system.

A lexicon of DNA modifications: their roles in embryo development and the germline (2018)
Journal Article
Zhu, Q., Stöger, R., & Alberio, R. (2018). A lexicon of DNA modifications: their roles in embryo development and the germline. Frontiers in Cell and Developmental Biology, 6, Article 24. https://doi.org/10.3389/fcell.2018.00024

5-methylcytosine (5mC) on CpG dinucleotides has been viewed as the major epigenetic modification in eukaryotes for a long time. Apart from 5mC, additional DNA modifications have been discovered in eukaryotic genomes. Many of these modifications are t... Read More about A lexicon of DNA modifications: their roles in embryo development and the germline.