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Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis

Ramos-Ibeas, Priscila; Sang, Fei; Zhu, Qifan; Wang, Walfred W.C.; Withey, Sarah; Klisch, Doris; Wood, Liam; Loose, Matt; Surani, M. Azim; Alberio, Ramiro

Authors

Priscila Ramos-Ibeas

FEI SANG Fei.Sang@nottingham.ac.uk
Research Fellow

Qifan Zhu

Walfred W.C. Wang

Sarah Withey

Doris Klisch

Liam Wood

MATTHEW LOOSE matt.loose@nottingham.ac.uk
Professor of Developmental and Computational Biology

M. Azim Surani

RAMIRO ALBERIO ramiro.alberio@nottingham.ac.uk
Professor of Developmental Biology



Abstract

High-resolution molecular programmes delineating the cellular foundations of mammalian embryogenesis have emerged recently. Similar analysis of human embryos is limited to pre-implantation stages, since early post-implantation embryos are largely inaccessible. Notwithstanding, we previously suggested conserved principles of pig and human early development. For further insight on pluripotent states and lineage delineation, we analysed pig embryos at single cell resolution. Here we show progressive segregation of inner cell mass and trophectoderm in early blastocysts, and of epiblast and hypoblast in late blastocysts. We show that following an emergent short naive pluripotent signature in early embryos, there is a protracted appearance of a primed signature in advanced embryonic stages. Dosage compensation with respect to the X-chromosome in females is attained via X-inactivation in late epiblasts. Detailed human-pig comparison is a basis towards comprehending early human development and a foundation for further studies of human pluripotent stem cell differentiation in pig interspecies chimeras.

Citation

Ramos-Ibeas, P., Sang, F., Zhu, Q., Wang, W. W., Withey, S., Klisch, D., …Alberio, R. (2019). Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis. Nature Communications, 10, https://doi.org/10.1038/s41467-019-08387-8

Journal Article Type Article
Acceptance Date Jan 4, 2019
Online Publication Date Jan 30, 2019
Publication Date Jan 30, 2019
Deposit Date Jan 16, 2019
Publicly Available Date Jan 30, 2019
Journal Nature Communications
Electronic ISSN 2041-1723
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 10
Article Number 500
DOI https://doi.org/10.1038/s41467-019-08387-8
Public URL https://nottingham-repository.worktribe.com/output/1477194
Publisher URL https://www.nature.com/articles/s41467-019-08387-8
Additional Information Received: 4 June 2018; Accepted: 4 January 2019; First Online: 30 January 2019; : The authors declare no competing interests.

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http://creativecommons.org/licenses/by/4.0/





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