Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis

Ramos-Ibeas, Priscila; Sang, Fei; Zhu, Qifan; Wang, Walfred W.C.; Withey, Sarah; Klisch, Doris; Wood, Liam; Loose, Matt; Surani, M. Azim; Alberio, Ramiro

Abstract

High-resolution molecular programmes delineating the cellular foundations of mammalian embryogenesis have emerged recently. Similar analysis of human embryos is limited to pre-implantation stages, since early post-implantation embryos are largely inaccessible. Notwithstanding, we previously suggested conserved principles of pig and human early development. For further insight on pluripotent states and lineage delineation, we analysed pig embryos at single cell resolution. Here we show progressive segregation of inner cell mass and trophectoderm in early blastocysts, and of epiblast and hypoblast in late blastocysts. We show that following an emergent short naive pluripotent signature in early embryos, there is a protracted appearance of a primed signature in advanced embryonic stages. Dosage compensation with respect to the X-chromosome in females is attained via X-inactivation in late epiblasts. Detailed human-pig comparison is a basis towards comprehending early human development and a foundation for further studies of human pluripotent stem cell differentiation in pig interspecies chimeras.

Citation

Ramos-Ibeas, P., Sang, F., Zhu, Q., Wang, W. W., Withey, S., Klisch, D., Wood, L., Loose, M., Surani, M. A., & Alberio, R. (2019). Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis. Nature Communications, 10, Article 500. https://doi.org/10.1038/s41467-019-08387-8

• Journal Article Type: Article
• Acceptance Date: Jan 4, 2019
• Online Publication Date: Jan 30, 2019
• Publication Date: Jan 30, 2019
• Deposit Date: Jan 16, 2019
• Publicly Available Date: Jan 30, 2019
• Journal: Nature Communications
• Electronic ISSN: 2041-1723
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 10
• Article Number: 500
• DOI: https://doi.org/10.1038/s41467-019-08387-8
• Public URL: https://nottingham-repository.worktribe.com/output/1477194
• Publisher URL: https://www.nature.com/articles/s41467-019-08387-8
• Additional Information: Received: 4 June 2018; Accepted: 4 January 2019; First Online: 30 January 2019; : The authors declare no competing interests.
• Contract Date: Jan 16, 2019