All Outputs (90)

Synthetic biology on acetogenic bacteria for highly efficient conversion of c1 gases to biochemicals (2020)
Journal Article
Jin, S., Bae, J., Song, Y., Pearcy, N., Shin, J., Kang, S., …Cho, B. K. (2020). Synthetic biology on acetogenic bacteria for highly efficient conversion of c1 gases to biochemicals. International Journal of Molecular Sciences, 21(20), Article 7639. https://doi.org/10.3390/ijms21207639

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Synthesis gas, which is mainly produced from fossil fuels or biomass gasification, consists of C1 gases such as carbon monoxide, carbon dioxide, and methane as well as hydrogen. Acetogenic bac... Read More about Synthetic biology on acetogenic bacteria for highly efficient conversion of c1 gases to biochemicals.

The glucosyltransferase activity of C. difficile toxin b is required for disease pathogenesis (2020)
Journal Article
Bilverstone, T. W., Garland, M., Cave, R. J., Kelly, M. L., Tholen, M., Bouley, D. M., …Melnyk, R. A. (2020). The glucosyltransferase activity of C. difficile toxin b is required for disease pathogenesis. PLoS Pathogens, 16(9), Article e1008852. https://doi.org/10.1371/journal.ppat.1008852

© 2020 Bilverstone et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source ar... Read More about The glucosyltransferase activity of C. difficile toxin b is required for disease pathogenesis.

Using singular perturbation theory to determine kinetic parameters in a non-standard coupled enzyme assay (2020)
Journal Article
Dalwadi, M. P., Orol, D., Walter, F., Minton, N. P., King, J. R., & Kovács, K. (2020). Using singular perturbation theory to determine kinetic parameters in a non-standard coupled enzyme assay. Journal of Mathematical Biology, 81(2), 649-690. https://doi.org/10.1007/s00285-020-01524-8

We investigate how to characterize the kinetic parameters of an aminotransaminase using a non-standard coupled (or auxiliary) enzyme assay, where the peculiarity arises for two reasons. First, one of the products of the auxiliary enzyme is a substrat... Read More about Using singular perturbation theory to determine kinetic parameters in a non-standard coupled enzyme assay.

A Sustainable Chemicals Manufacturing Paradigm Using CO2 and Renewable H2 (2020)
Journal Article
Bommareddy, R. R., Wang, Y., Pearcy, N., Hayes, M., Lester, E., Minton, N. P., & Conradie, A. V. (2020). A Sustainable Chemicals Manufacturing Paradigm Using CO2 and Renewable H2. iScience, 23(6), Article 101218. https://doi.org/10.1016/j.isci.2020.101218

© 2020 The Author(s) The chemical industry must decarbonize to align with UN Sustainable Development Goals. A shift toward circular economies makes CO2 an attractive feedstock for producing chemicals, provided renewable H2 is available through techno... Read More about A Sustainable Chemicals Manufacturing Paradigm Using CO2 and Renewable H2.

A gold standard, CRISPR/Cas9-based complementation strategy reliant on 24 nucleotide bookmark sequences (2020)
Journal Article
Seys, F. M., Rowe, P., Bolt, E. L., Humphreys, C. M., & Minton, N. P. (2020). A gold standard, CRISPR/Cas9-based complementation strategy reliant on 24 nucleotide bookmark sequences. Genes, 11(4), Article 458. https://doi.org/10.3390/genes11040458

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Phenotypic complementation of gene knockouts is an essential step in establishing function. Here, we describe a simple strategy for ‘gold standard’ complementation in which the mutant allele i... Read More about A gold standard, CRISPR/Cas9-based complementation strategy reliant on 24 nucleotide bookmark sequences.

A genome-wide approach for identification and characterisation of metabolite-inducible systems (2020)
Journal Article
Hanko, E. K. R., Paiva, A. C., Jonczyk, M., Abbott, M., Minton, N. P., & Malys, N. (2020). A genome-wide approach for identification and characterisation of metabolite-inducible systems. Nature Communications, 11(1), Article 1213. https://doi.org/10.1038/s41467-020-14941-6

© 2020, The Author(s). Inducible gene expression systems are vital tools for the advancement of synthetic biology. Their application as genetically encoded biosensors has the potential to contribute to diagnostics and to revolutionise the field of mi... Read More about A genome-wide approach for identification and characterisation of metabolite-inducible systems.

A complete genome sequence of Cupriavidus necator H16 (DSM 428) (2019)
Journal Article
Little, G. T., Ehsaan, M., Arenas-López, C., Jawed, K., Winzer, K., Kovacs, K., & Minton, N. P. (2019). A complete genome sequence of Cupriavidus necator H16 (DSM 428). Microbiology Resource Announcements, 8(37), 1-2. https://doi.org/10.1128/MRA.00814-19

The hydrogen-utilizing strain Cupriavidus necator H16 (DSM 428) was sequenced using a combination of PacBio and Illumina sequencing. Annotation of this strain reveals 6,543 protein-coding genes, 263 pseudogenes, 64 tRNA genes, and 15 rRNA genes.

Engineering Geobacillus thermoglucosidasius for direct utilisation of holocellulose from wheat straw (2019)
Journal Article
Bashir, Z., Sheng, L., Anil, A., Lali, A., Minton, N. P., & Zhang, Y. (2019). Engineering Geobacillus thermoglucosidasius for direct utilisation of holocellulose from wheat straw. Biotechnology for Biofuels, 12(1), Article 199. https://doi.org/10.1186/s13068-019-1540-6

Background: A consolidated bioprocessing (CBP), where lignocellulose is converted into the desired product(s) in a single fermentative step without the addition of expensive degradative enzymes, represents the ideal solution of renewable routes to ch... Read More about Engineering Geobacillus thermoglucosidasius for direct utilisation of holocellulose from wheat straw.

The carbonic anhydrase of Clostridium autoethanogenum represents a new subclass of β-carbonic anhydrases (2019)
Journal Article
Pander, B., Harris, G., Scott, D. J., Winzer, K., Köpke, M., Simpson, S. D., …Henstra, A. M. (2019). The carbonic anhydrase of Clostridium autoethanogenum represents a new subclass of β-carbonic anhydrases. Applied Microbiology and Biotechnology, 103(17), 7275-7286. https://doi.org/10.1007/s00253-019-10015-w

Carbonic anhydrase catalyses the interconversion of carbon dioxide and water to bicarbonate and protons. It was unknown if the industrial relevant acetogen Clostridium autoethanogenum possesses these enzymes. We identified two putative carbonic anhyd... Read More about The carbonic anhydrase of Clostridium autoethanogenum represents a new subclass of β-carbonic anhydrases.

Phosphorylation and functionality of CdtR in Clostridium difficile (2019)
Journal Article
Bilverstone, T., Minton, N., & Kuehne, S. (2019). Phosphorylation and functionality of CdtR in Clostridium difficile. Anaerobe, 58, 103-109. https://doi.org/10.1016/j.anaerobe.2019.102074

The production of TcdA, TcdB and CDT in Clostridium difficile PCR ribotype 027, is regulated by the two-component system response regulator CdtR. Despite this, little is known about the signal transduction pathway leading to the activation of CdtR. I... Read More about Phosphorylation and functionality of CdtR in Clostridium difficile.

A novel conjugal donor strain for improved DNA transfer into Clostridium spp. (2019)
Journal Article
Woods, C., Humphreys, C. M., Rodrigues, R. M., Ingle, P., Rowe, P., Henstra, A. M., …Minton, N. P. (2019). A novel conjugal donor strain for improved DNA transfer into Clostridium spp. Anaerobe, 59, 184-191. https://doi.org/10.1016/j.anaerobe.2019.06.020

© 2019 The Authors Importance: The ability to transfer genetic material into a target organism is crucial for the development of a wide range of targeted genetic manipulation techniques. Overcoming the organisms’ native restriction systems which targ... Read More about A novel conjugal donor strain for improved DNA transfer into Clostridium spp..

The genetic basis of 3-hydroxypropanoate metabolism in Cupriavidus necator H16 (2019)
Journal Article
Arenas-López, C., Locker, J., Orol, D., Walter, F., Busche, T., Kalinowski, J., …Winzer, K. (2019). The genetic basis of 3-hydroxypropanoate metabolism in Cupriavidus necator H16. Biotechnology for Biofuels, 12, Article 150. https://doi.org/10.1186/s13068-019-1489-5

Background
3-Hydroxypropionic acid (3-HP) is a promising platform chemical with various industrial applications. Several metabolic routes to produce 3-HP from organic substrates such as sugars or glycerol have been implemented in yeast, enterobacter... Read More about The genetic basis of 3-hydroxypropanoate metabolism in Cupriavidus necator H16.

RiboCas: A Universal CRISPR-Based Editing Tool for Clostridium (2019)
Journal Article
Cañadas, I. C., Groothuis, D., Zygouropoulou, M., Rodrigues, R., & Minton, N. P. (2019). RiboCas: A Universal CRISPR-Based Editing Tool for Clostridium. ACS Synthetic Biology, 8(6), 1379-1390. https://doi.org/10.1021/acssynbio.9b00075

Members of the genus Clostridium represent a diverse assemblage of species exhibiting both medical and industrial importance. Deriving both a greater understanding of their biology, while at the same time enhancing their exploitable properties, requi... Read More about RiboCas: A Universal CRISPR-Based Editing Tool for Clostridium.

Generation of a fully erythromycin-sensitive strain of Clostridioides difficile using a novel CRISPR-Cas9 genome editing system (2019)
Journal Article
Ingle, P., Groothuis, D., Rowe, P., Huang, H., Cockayne, A., Kuehne, S. A., …Minton, N. P. (2019). Generation of a fully erythromycin-sensitive strain of Clostridioides difficile using a novel CRISPR-Cas9 genome editing system. Scientific Reports, 9, Article 8123. https://doi.org/10.1038/s41598-019-44458-y

© 2019, The Author(s). Understanding the molecular pathogenesis of Clostridioides difficile has relied on the use of ermB-based mutagens in erythromycin-sensitive strains. However, the repeated subcultures required to isolate sensitive variants can l... Read More about Generation of a fully erythromycin-sensitive strain of Clostridioides difficile using a novel CRISPR-Cas9 genome editing system.

Genome-scale model of C. autoethanogenum reveals optimal bioprocess conditions for high-value chemical production from carbon monoxide (2019)
Journal Article
Norman, R. O., Millat, T., Schatschneider, S., Henstra, A. M., Breitkopf, R., Pander, B., …Hodgman, C. (2019). Genome-scale model of C. autoethanogenum reveals optimal bioprocess conditions for high-value chemical production from carbon monoxide. Engineering Biology, 3(2), 32-40. https://doi.org/10.1049/enb.2018.5003

Clostridium autoethanogenum is an industrial microbe used for the commercial-scale production of ethanol from carbon monoxide. While significant progress has been made in the attempted diversification of this bioprocess, further improvements are desi... Read More about Genome-scale model of C. autoethanogenum reveals optimal bioprocess conditions for high-value chemical production from carbon monoxide.

Engineering of vitamin prototrophy in Clostridium ljungdahlii and Clostridium autoethanogenum (2019)
Journal Article
Annan, F. J., Al-Sinawi, B., Humphreys, C. M., Norman, R., Winzer, Köpke, M., …Henstra, A. M. (2019). Engineering of vitamin prototrophy in Clostridium ljungdahlii and Clostridium autoethanogenum. Applied Microbiology and Biotechnology, 103(11), 4633–4648. https://doi.org/10.1007/s00253-019-09763-6

Clostridium autoethanogenum and Clostridium ljungdahlii are physiologically and genetically very similar strict anaerobic acetogens capable of growth on carbon monoxide as sole carbon source. While exact nutritional requirements have not been reporte... Read More about Engineering of vitamin prototrophy in Clostridium ljungdahlii and Clostridium autoethanogenum.

Homologous overexpression of hydrogenase and glycerol dehydrogenase in Clostridium pasteurianum to enhance hydrogen production from crude glycerol (2019)
Journal Article
Sarma, S., Ortega, D., Minton, N. P., Dubey, V. K., & Moholkar, V. S. (2019). Homologous overexpression of hydrogenase and glycerol dehydrogenase in Clostridium pasteurianum to enhance hydrogen production from crude glycerol. Bioresource Technology, 284, 168-177. https://doi.org/10.1016/j.biortech.2019.03.074

This study reports engineering of a hypertransformable variant of C. pasteurianum for bioconversion of glycerol into hydrogen (H2). A functional glycerol-triggered hydrogen pathway was engineered based on two approaches: (1) increasing product yield... Read More about Homologous overexpression of hydrogenase and glycerol dehydrogenase in Clostridium pasteurianum to enhance hydrogen production from crude glycerol.

Gsmodutils: a python based framework for test-driven genome scale metabolic model development (2019)
Journal Article
Gilbert, J., Pearcy, N., Norman, R., Millat, T., Winzer, K., King, J., …Twycross, J. (2019). Gsmodutils: a python based framework for test-driven genome scale metabolic model development. Bioinformatics, 35(18), 3397-3403. https://doi.org/10.1093/bioinformatics/btz088

© 2019 The Author(s) 2019. Published by Oxford University Press. Motivation: Genome scale metabolic models (GSMMs) are increasingly important for systems biology and metabolic engineering research as they are capable of simulating complex steady-stat... Read More about Gsmodutils: a python based framework for test-driven genome scale metabolic model development.

CRISPR-Cas9D10A nickase-assisted base editing in the solvent producer Clostridium beijerinckii (2019)
Journal Article
Li, Q., Seys, F. M., Minton, N. P., Yang, J., Jiang, Y., Jiang, W., & Yang, S. (2019). CRISPR-Cas9D10A nickase-assisted base editing in the solvent producer Clostridium beijerinckii. Biotechnology and Bioengineering, 116(6), 1475-1483. https://doi.org/10.1002/bit.26949

Clostridium beijerinckii is a potentially important industrial microorganism as it can synthesize valuable chemicals and fuels from various carbon sources. The establishment of convenient to use, effective gene tools with which the organism can be ra... Read More about CRISPR-Cas9D10A nickase-assisted base editing in the solvent producer Clostridium beijerinckii.

The Rnf complex is an energy-coupled transhydrogenase essential to reversibly link cellular NADH and ferredoxin pools in the acetogen acetobacterium woodii (2018)
Journal Article
Westphal, L., Wiechmann, A., Baker, J., Minton, N. P., & Müller, V. (2018). The Rnf complex is an energy-coupled transhydrogenase essential to reversibly link cellular NADH and ferredoxin pools in the acetogen acetobacterium woodii. Journal of Bacteriology, 200(21), https://doi.org/10.1128/JB.00357-18

© 2018 American Society for Microbiology. The Rnf complex is a respiratory enzyme that catalyzes the oxidation of reduced ferredoxin to the reduction of NAD + , and the negative free energy change of this reaction is used to generate a transmembrane... Read More about The Rnf complex is an energy-coupled transhydrogenase essential to reversibly link cellular NADH and ferredoxin pools in the acetogen acetobacterium woodii.