Base editing enables duplex point mutagenesis in Clostridium autoethanogenum at the price of numerous off-target mutations

Seys, François M.; Humphreys, Christopher M.; Tomi-Andrino, Claudio; Li, Qi; Millat, Thomas; Yang, Sheng; Minton, Nigel P.

doi:10.3389/fbioe.2023.1211197

All Outputs (2)

Endogenous CRISPR/Cas systems for genome engineering in the acetogens Acetobacterium woodii and Clostridium autoethanogenum (2023)
Journal Article

Acetogenic bacteria can play a major role in achieving Net Zero through their ability to convert CO2 into industrially relevant chemicals and fuels. Full exploitation of this potential will be reliant on effective metabolic engineering tools, such as... Read More about Endogenous CRISPR/Cas systems for genome engineering in the acetogens Acetobacterium woodii and Clostridium autoethanogenum.

Base editing enables duplex point mutagenesis in Clostridium autoethanogenum at the price of numerous off-target mutations (2023)
Journal Article

Base editors are recent multiplex gene editing tools derived from the Cas9 nuclease of Streptomyces pyogenes. They can target and modify a single nucleotide in the genome without inducing double-strand breaks (DSB) of the DNA helix. As such, they hol... Read More about Base editing enables duplex point mutagenesis in Clostridium autoethanogenum at the price of numerous off-target mutations.