Profiling Humoral Immune Responses to Clostridium difficile-Specific Antigens by Protein Microarray Analysis

Negm, Ola H.; Hamed, Mohamed R.; Dilnot, Elizabeth M.; Shone, Clifford C.; Marszalowska, Izabela; Lynch, Mark; Loscher, Christine E.; Edwards, Laura J.; Tighe, Patrick J.; Wilcox, Mark H.; Monaghan, Tanya M.

doi:10.1128/CVI.00190-15

All Outputs (2)

A protein microarray assay for serological determination of antigen-specific antibody responses following Clostridium difficile infection (2018)
Journal Article

We provide a detailed overview of a novel high-throughput protein microarray assay for the determination of anti-C. difficile antibody levels in human sera and in separate preparations of polyclonal IVIg. The protocol describes the methodological ste... Read More about A protein microarray assay for serological determination of antigen-specific antibody responses following Clostridium difficile infection.

Profiling Humoral Immune Responses to Clostridium difficile-Specific Antigens by Protein Microarray Analysis (2015)
Journal Article

Clostridium difficile is an anaerobic, Gram-positive, and spore-forming bacterium that is the leading worldwide infective cause of hospital-acquired and antibiotic-associated diarrhea. Several studies have reported associations between humoral immuni... Read More about Profiling Humoral Immune Responses to Clostridium difficile-Specific Antigens by Protein Microarray Analysis.