All Outputs (136)

Effect of surfactant on Pseudomonas aeruginosa colonization of polymer microparticles and flat films (2018)
Journal Article
Hüsler, A., Haas, S., Parry, L., Romero, M., Nisisako, T., Williams, P., Wildman, R. D., & Alexander, M. R. (2018). Effect of surfactant on Pseudomonas aeruginosa colonization of polymer microparticles and flat films. RSC Advances, 8(28), https://doi.org/10.1039/c8ra01491d

Micro- and nanoparticles are of great interest because of their potential for trafficking into the body for applications such as low-fouling coatings on medical devices, drug delivery in pharmaceutics and cell carriers in regenerative medicine strate... Read More about Effect of surfactant on Pseudomonas aeruginosa colonization of polymer microparticles and flat films.

Electrochemically stimulating developments in bioelectronic medicine (2018)
Journal Article
Sanjuan-Alberte, P., Alexander, M. R., Hague, R. J., & Rawson, F. J. (2018). Electrochemically stimulating developments in bioelectronic medicine. Bioelectronic Medicine, 4(1), https://doi.org/10.1186/s42234-018-0001-z

Cellular homeostasis is in part controlled by biological generated electrical activity. By interfacing biology with electronic devices this electrical activity can be modulated to actuate cellular behaviour. There are current limitations in merging e... Read More about Electrochemically stimulating developments in bioelectronic medicine.

3D extrusion printing of high drug loading immediate release paracetamol tablets (2018)
Journal Article
Khaled, S. A., Alexander, M. R., Wildman, R. D., Wallace, M. J., Sharpe, S., Yoo, J., & Roberts, C. J. (2018). 3D extrusion printing of high drug loading immediate release paracetamol tablets. International Journal of Pharmaceutics, 538(1-2), 223-230. https://doi.org/10.1016/j.ijpharm.2018.01.024

The manufacture of immediate release high drug loading paracetamol oral tablets was achieved using an extrusion based 3D printer from a premixed water based paste formulation. The 3D printed tablets demonstrate that a very high drug (paracetamol) loa... Read More about 3D extrusion printing of high drug loading immediate release paracetamol tablets.

Identification of novel ‘inks’ for 3D printing using high throughput screening: bioresorbable photocurable polymers for controlled drug delivery (2018)
Journal Article
Louzao, I., Koch, B., Taresco, V., Ruiz Cantu, L., Irvine, D. J., Roberts, C. J., Tuck, C. J., Alexander, C., Hague, R. J., Wildman, R. D., & Alexander, M. R. (in press). Identification of novel ‘inks’ for 3D printing using high throughput screening: bioresorbable photocurable polymers for controlled drug delivery. ACS Applied Materials and Interfaces, 10(8), https://doi.org/10.1021/acsami.7b15677

A robust discovery methodology is presented to identify novel biomaterials suitable for 3D printing. Currently the application of Additive Manufacturing is limited by the availability of functional inks, especially in the area of biomaterials-this me... Read More about Identification of novel ‘inks’ for 3D printing using high throughput screening: bioresorbable photocurable polymers for controlled drug delivery.

Prediction of Broad-Spectrum Pathogen Attachment to Coating Materials for Biomedical Devices (2018)
Journal Article
Dundas, A. A., Mikulskis, P., Hook, A., Dundas, A., Irvine, D., Sanni, O., Anderson, D., Langer, R., Alexander, M. R., Williams, P., & Winkler, D. A. (2018). Prediction of Broad-Spectrum Pathogen Attachment to Coating Materials for Biomedical Devices. ACS Applied Materials and Interfaces, 10(1), 139-149. https://doi.org/10.1021/acsami.7b14197

© 2017 American Chemical Society. Bacterial infections in healthcare settings are a frequent accompaniment to both routine procedures such as catheterization and surgical site interventions. Their impact is becoming even more marked as the numbers of... Read More about Prediction of Broad-Spectrum Pathogen Attachment to Coating Materials for Biomedical Devices.

Synthetic light-curable polymeric materials provide a supportive niche for dental pulp stem cells (2017)
Journal Article
Vining, K. H., Scherba, J. C., Bever, A., Alexander, M. R., Celiz, A. D., & Mooney, D. J. (2018). Synthetic light-curable polymeric materials provide a supportive niche for dental pulp stem cells. Advanced Materials, 30(4), Article 1704486. https://doi.org/10.1002/adma.201704486

Dental disease annually affects billions of patients, and while regenerative dentistry aims to heal dental tissue after injury, existing polymeric restorative materials, or fillings, do not directly participate in the healing process in a bioinstruct... Read More about Synthetic light-curable polymeric materials provide a supportive niche for dental pulp stem cells.

The 3D OrbiSIMS—label-free metabolic imaging with subcellular lateral resolution and high mass-resolving power (2017)
Journal Article
Passarelli, M. K., Pirkl, A., Moellers, R., Grinfeld, D., Kollmer, F., Havelund, R., Newman, C. F., Marshall, P. S., Arlinghaus, H., Alexander, M. R., West, A., Horning, S., Niehuis, E., Makarov, A., Dollery, C. T., & Gilmore, I. S. (2017). The 3D OrbiSIMS—label-free metabolic imaging with subcellular lateral resolution and high mass-resolving power. Nature Methods, 14(12), 1175-1183. https://doi.org/10.1038/nmeth.4504

© 2017 Nature America, Inc., part of Springer Nature. All rights reserved. We report the development of a 3D OrbiSIMS instrument for label-free biomedical imaging. It combines the high spatial resolution of secondary ion mass spectrometry (SIMS; unde... Read More about The 3D OrbiSIMS—label-free metabolic imaging with subcellular lateral resolution and high mass-resolving power.

Intracellular drug uptake: a comparison of single cell measurements using ToF-SIMS imaging and quantification from cell populations with LC/MS/MS (2017)
Journal Article
Newman, C. F., Havelund, R., Passarelli, M. K., Marshall, P. S., Francis, I., West, A., Alexander, M. R., Gilmore, I. S., & Dollery, C. T. (2017). Intracellular drug uptake: a comparison of single cell measurements using ToF-SIMS imaging and quantification from cell populations with LC/MS/MS. Analytical Chemistry, 89(22), 11944-11953. https://doi.org/10.1021/acs.analchem.7b01436

ToF-SIMS is a label-free imaging method that has been shown to enable imaging of amiodarone in single rat macrophage (NR8383) cells. In this study, we show that the method extends to three other cell lines relevant to drug discovery: human embryonic... Read More about Intracellular drug uptake: a comparison of single cell measurements using ToF-SIMS imaging and quantification from cell populations with LC/MS/MS.

Water contact angle is not a good predictor of biological responses to materials (2017)
Journal Article
Alexander, M. R., & Williams, P. (in press). Water contact angle is not a good predictor of biological responses to materials. Biointerphases, 12(2), Article 02C201. https://doi.org/10.1116/1.4989843

Often the view is expressed that water contact angle (WCA) or other wettability/surface energy measurements made on a material surface can be used to predict cellular attachment to materials, e.g., bacteria attach to hydrophobic surfaces. In this art... Read More about Water contact angle is not a good predictor of biological responses to materials.

Versatile routes to functional RAFT chain transfer agents through the Passerini multicomponent reaction (2017)
Journal Article
Pearce, A. K., Travanut, A., Couturaud, B., Taresco, V., Howdle, S. M., Alexander, M. R., & Alexander, C. (2017). Versatile routes to functional RAFT chain transfer agents through the Passerini multicomponent reaction. ACS Macro Letters, 6(7), 781-785. https://doi.org/10.1021/acsmacrolett.7b00415

The widespread adoption of RAFT polymerization stems partly from the ease and utility of installing a functional chain transfer agent onto the ends of the generated polymer chains. In parallel, the Passerini multicomponent reaction offers great versa... Read More about Versatile routes to functional RAFT chain transfer agents through the Passerini multicomponent reaction.

3D printing of tablets using inkjet with UV photoinitiation (2017)
Journal Article
Clark, E. A., Alexander, M. R., Irvine, D. J., Roberts, C. J., Wallace, M. J., Sharpe, S., Yoo, J., Hague, R. J., Tuck, C. J., & Wildman, R. D. (2017). 3D printing of tablets using inkjet with UV photoinitiation. International Journal of Pharmaceutics, 529(1-2), 523-530. https://doi.org/10.1016/j.ijpharm.2017.06.085

Additive manufacturing (AM) offers significant potential benefits in the field of drug delivery and pharmaceutical/medical device manufacture. Of AM processes, 3D inkjet printing enables precise deposition of a formulation, whilst offering the potent... Read More about 3D printing of tablets using inkjet with UV photoinitiation.

3D inkjet printing of tablets exploiting bespoke complex geometries for controlled and tuneable drug release (2017)
Journal Article
Kyobula, M., Adedeji, A., Alexander, M. R., Saleh, E., Wildman, R. D., Ashcroft, I., Gellert, P. R., & Roberts, C. J. (2017). 3D inkjet printing of tablets exploiting bespoke complex geometries for controlled and tuneable drug release. Journal of Controlled Release, 261, 207-215. https://doi.org/10.1016/j.jconrel.2017.06.025

A hot melt 3D inkjet printing method with the potential to manufacture formulations in complex and adaptable geometries for the controlled loading and release of medicines is presented. This first use of a precisely controlled solvent free inkjet pri... Read More about 3D inkjet printing of tablets exploiting bespoke complex geometries for controlled and tuneable drug release.

Image based machine learning for identification of macrophage subsets (2017)
Journal Article
Rostam, H., Reynolds, P. M., Alexander, M. R., Gadegaard, N., & Ghaemmaghami, A. M. (2017). Image based machine learning for identification of macrophage subsets. Scientific Reports, 7(1), Article 3521. https://doi.org/10.1038/s41598-017-03780-z

Macrophages play a crucial rule in orchestrating immune responses against pathogens and foreign materials. Macrophages have remarkable plasticity in response to environmental cues and are able to acquire a spectrum of activation status, best exemplif... Read More about Image based machine learning for identification of macrophage subsets.

Development and characterization of a stable adhesive bond between a poly(dimethylsiloxane) catheter material and a bacterial biofilm resistant acrylate polymer coating (2017)
Journal Article
Tyler, B. J., Hook, A. L., Pelster, A., Williams, P., Alexander, M. R., & Arlinghaus, H. F. (2017). Development and characterization of a stable adhesive bond between a poly(dimethylsiloxane) catheter material and a bacterial biofilm resistant acrylate polymer coating. Biointerphases, 12(2), Article 02C412. https://doi.org/10.1116/1.4984011

Catheter associated urinary tract infections (CA-UTIs) are the most common health related infections world wide, contributing significantly to patient morbidity and mortality and increased health care costs. To reduce the incidence of these infection... Read More about Development and characterization of a stable adhesive bond between a poly(dimethylsiloxane) catheter material and a bacterial biofilm resistant acrylate polymer coating.

Bioprinting Using Mechanically Robust Core–Shell Cell-Laden Hydrogel Strands (2017)
Journal Article
Mistry, P., Aied, A., Alexander, M. R., Shakesheff, K., Bennett, A., & Yang, J. (2017). Bioprinting Using Mechanically Robust Core–Shell Cell-Laden Hydrogel Strands. Macromolecular Bioscience, 17(6), Article 1600472. https://doi.org/10.1002/mabi.201600472

© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim The strand material in extrusion-based bioprinting determines the microenvironments of the embedded cells and the initial mechanical properties of the constructs. One unmet challenge is the combinat... Read More about Bioprinting Using Mechanically Robust Core–Shell Cell-Laden Hydrogel Strands.

Making Silicone Rubber Highly Resistant to Bacterial Attachment Using Thiol-ene Grafting (2016)
Journal Article
Magennis, E. P., Hook, A. L., Williams, P., & Alexander, M. R. (2016). Making Silicone Rubber Highly Resistant to Bacterial Attachment Using Thiol-ene Grafting. ACS Applied Materials and Interfaces, 8(45), 30780-30787. https://doi.org/10.1021/acsami.6b10986

Biomedical devices are indispensable in modern medicine yet offer surfaces that promote bacterial attachment and biofilm formation, resulting in acute and chronic healthcare-associated infections. We have developed a simple method to graft acrylates... Read More about Making Silicone Rubber Highly Resistant to Bacterial Attachment Using Thiol-ene Grafting.

Application of Targeted Molecular and Material Property Optimization to Bacterial Attachment-Resistant (Meth)acrylate Polymers (2016)
Journal Article
Adlington, K., Nguyen, N. T., Eaves, E., Yang, J., Chang, C. Y., Li, J., Gower, A. L., Stimpson, A., Anderson, D. G., Langer, R., Davies, M. C., Hook, A. L., Williams, P., Alexander, M. R., & Irvine, D. J. (2016). Application of Targeted Molecular and Material Property Optimization to Bacterial Attachment-Resistant (Meth)acrylate Polymers. Biomacromolecules, 17(9), 2830-2838. https://doi.org/10.1021/acs.biomac.6b00615

© 2016 American Chemical Society. Developing medical devices that resist bacterial attachment and subsequent biofilm formation is highly desirable. In this paper, we report the optimization of the molecular structure and thus material properties of a... Read More about Application of Targeted Molecular and Material Property Optimization to Bacterial Attachment-Resistant (Meth)acrylate Polymers.

Development, printability and post-curing studies of formulations of materials resistant to microbial attachment for use in inkjet based 3D printing (2016)
Journal Article
Begines, B., Hook, A. L., Alexander, M. R., Tuck, C. J., & Wildman, R. D. (2016). Development, printability and post-curing studies of formulations of materials resistant to microbial attachment for use in inkjet based 3D printing. Rapid Prototyping Journal, 22(5), 835-841. https://doi.org/10.1108/RPJ-11-2015-0175

Purpose: This paper aims to print 3D structures from polymers that resist bacterial attachment by reactive jetting of acrylate monomers.
Design/methodology/approach: The first step towards printing was ink development. Inks were characterised to car... Read More about Development, printability and post-curing studies of formulations of materials resistant to microbial attachment for use in inkjet based 3D printing.

Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion (2016)
Journal Article
Hammad, M., Rao, W., Smith, J. G. W., Anderson, D. G., Langer, R., Young, L. E., Barrett, D. A., Davies, M. C., Denning, C., & Alexander, M. R. (2016). Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion. Biomaterials Science, 4(9), 1381-1391. https://doi.org/10.1039/c6bm00214e

Improved biomaterials are required for application in regenerative medicine, biosensing, and as medical devices. The response of cells to the chemistry of polymers cultured in media is generally regarded as being dominated by proteins adsorbed to the... Read More about Identification of polymer surface adsorbed proteins implicated in pluripotent human embryonic stem cell expansion.

The impact of surface chemistry modification on macrophage polarisation (2016)
Journal Article
Rostam, H., Singh, S., Salazar, F., Magennis, P., Hook, A. L., Singh, T., Vrana, N., Alexander, M. R., & Ghaemmaghami, A. M. (2016). The impact of surface chemistry modification on macrophage polarisation. Immunobiology, 221(11), 1237-1246. https://doi.org/10.1016/j.imbio.2016.06.010

Macrophages are innate immune cells that have a central role in combating infection and maintaining tissue homeostasis. They exhibit remarkable plasticity in response to environmental cues. At either end of a broad activation spectrum are pro-inflamm... Read More about The impact of surface chemistry modification on macrophage polarisation.