Research Repository

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Cross-chemistry leads to product diversity from atromentin synthetases in Aspergilli from section Nigri (2018)
Journal Article
Geib, E., Baldeweg, F., Doerfer, M., Nett, M., & Brock, M. (2018). Cross-chemistry leads to product diversity from atromentin synthetases in Aspergilli from section Nigri. Cell Chemical Biology, doi:10.1016/j.chembiol.2018.10.021

Nonribosomal peptide synthetase (NRPS)-like enzymes catalyse the non-oxidative homodimerisation of aromatic α-keto acids, but the exact reaction mechanism is unknown. The furanone-forming thioesterase domain of the Aspergillus terreus aspulvinone E s... Read More

ATNT: an enhanced system for expression of polycistronic secondary metabolite gene clusters in Aspergillus niger (2017)
Journal Article
Geib, E., & Brock, M. (in press). ATNT: an enhanced system for expression of polycistronic secondary metabolite gene clusters in Aspergillus niger. Fungal Biology and Biotechnology, 4(1), doi:10.1186/s40694-017-0042-1. ISSN 2054-3085

Background: Fungi are treasure chests for yet unexplored natural products. However, exploitation of their real potential remains difficult as a significant proportion of biosynthetic gene clusters appears silent under standard laboratory conditions.... Read More

Candida albicans Hap43 domains are required under iron starvation but not excess (2017)
Journal Article
Skrahina, V., Brock, M., Hube, B., & Brunke, S. (2017). Candida albicans Hap43 domains are required under iron starvation but not excess. Frontiers in Microbiology, 8, doi:10.3389/fmicb.2017.02388. ISSN 1664-302X

Iron availability is a central factor in infections, since iron is a critical micronutrient for all living organisms. The host employs both iron limitation and toxicity strategies to control microbial growth, and successful pathogens are able to tigh... Read More

Persistence within dendritic cells marks an antifungal evasion and dissemination strategy of Aspergillus terreus (2017)
Journal Article
Hsieh, S., Kurzai, O., & Brock, M. (2017). Persistence within dendritic cells marks an antifungal evasion and dissemination strategy of Aspergillus terreus. Scientific Reports, 7, doi:10.1038/s41598-017-10914-w. ISSN 2045-2322

Aspergillus terreus is an airborne human fungal pathogen causing life-threatening invasive aspergillosis in immunocompromised patients. In contrast to Aspergillus fumigatus, A. terreus infections are associated with high dissemination rates and poor... Read More

A highly conserved basidiomycete peptide synthetase produces a trimeric hydroxamate siderophore (2017)
Journal Article
Brandenburger, E., Gressler, M., Leonhardt, R., Lackner, G., Habel, A., Hertweck, C., …Hoffmeister, D. (in press). A highly conserved basidiomycete peptide synthetase produces a trimeric hydroxamate siderophore. Applied and Environmental Microbiology, doi:10.1128/AEM.01478-17. ISSN 0099-2240

The model white-rot basidiomycete Ceriporiopsis (Gelatoporia) subvermispora B encodes putative natural product biosynthesis genes. Among them is the gene for the seven-domain nonribosomal peptide synthetase CsNPS2. It is a member of the as-yet unchar... Read More

Lipid components of bile increase the protective effect of conjugated bile salts against antifungal drugs (2017)
Journal Article
Hsieh, S., & Brock, M. (in press). Lipid components of bile increase the protective effect of conjugated bile salts against antifungal drugs. Fungal Biology, doi:10.1016/j.funbio.2017.08.002. ISSN 1878-6146

Fungi and bacteria can persist in the human gall bladder. Previous studies have shown that bile protects Candida albicans in this cryptic host niche from antifungals, providing a reservoir for intestinal re-colonization after discontinuation of antif... Read More

Encapsulation of antifungals in micelles protects Candida albicans during gall-bladder infection (2017)
Journal Article
Hsieh, S., Brunke, S., & Brock, M. (2017). Encapsulation of antifungals in micelles protects Candida albicans during gall-bladder infection. Frontiers in Microbiology, 8, doi:10.3389/fmicb.2017.00117. ISSN 1664-302X

Candida albicans is a dimorphic fungus that colonizes human mucosal surfaces with the potential to cause life-threatening invasive candidiasis. Studies on systemic candidiasis in a murine infection model using in vivo real-time bioluminescence imagin... Read More

Widespread inter- and intra-domain horizontal gene transfer of d-amino acid metabolism enzymes in eukaryotes (2016)
Journal Article
Naranjo-Ortíz, M. A., Brock, M., Brunke, S., Hube, B., Marcet-Houben, M., & Gabaldón, T. (2016). Widespread inter- and intra-domain horizontal gene transfer of d-amino acid metabolism enzymes in eukaryotes. Frontiers in Microbiology, 7, doi:10.3389/fmicb.2016.02001. ISSN 1664-302X

Analysis of the growing number of available fully-sequenced genomes has shown that Horizontal Gene Transfer (HGT) in eukaryotes is more common than previously thought. It has been proposed that genes with certain functions may be more prone to HGT th... Read More

A non-canonical melanin biosynthesis pathway protects Aspergillus terreus conidia from environmental stress (2016)
Journal Article
Geib, E., Gressler, M., Viediernikova, I., Hillmann, F., Jacobsen, I. D., Nietzsche, S., …Brock, M. (2016). A non-canonical melanin biosynthesis pathway protects Aspergillus terreus conidia from environmental stress. Cell Chemical Biology, 23(5), doi:10.1016/j.chembiol.2016.03.014

Pro- and eukaryotes produce melanin for protection from environmental stress or as virulence determinant. The human pathogenic fungus Aspergillus fumigatus and related Ascomycetes produce dihydroxynaphthalene (DHN) melanin in conidia, which is essent... Read More

Infection-mediated priming of phagocytes protects against lethal secondary Aspergillus fumigatus challenge (2016)
Journal Article
Savers, A., Rasid, O., Parlato, M., Brock, M., Jouvion, G., Ryffel, B., …Ibrahim-Granet, O. (2016). Infection-mediated priming of phagocytes protects against lethal secondary Aspergillus fumigatus challenge. PLoS ONE, 11(4), doi:10.1371/journal.pone.0153829. ISSN 1932-6203

Phagocytes restrict the germination of Aspergillus fumigatus conidia and prevent the establishment of invasive pulmonary aspergillosis in immunecompetent mice. Here we report that immunecompetent mice recovering from a primary A. fumigatus challenge... Read More

Phytotoxin production in Aspergillus terreus is regulated by independent environmental signals (2015)
Journal Article
Gressler, M., Meyer, F., Heine, D., Hortschansky, P., Hertweck, C., & Brock, M. (2015). Phytotoxin production in Aspergillus terreus is regulated by independent environmental signals. eLife, 4, doi:10.7554/eLife.07861.001. ISSN 2050-084X

Secondary metabolites have a great potential as pharmaceuticals, but there are only a few examples where regulation of gene cluster expression has been correlated with ecological and physiological relevance for the producer. Here, signals, mediators,... Read More

Phytotoxin production in Aspergillus terreus is regulated by independent environmental signals. (2015)
Journal Article
Gressler, M., Meyer, F., Heine, D., Hortschansky, P., Hertweck, C., & Brock, M. (2015). Phytotoxin production in Aspergillus terreus is regulated by independent environmental signals. eLife, 4, doi:10.7554/eLife.07861

Secondary metabolites have a great potential as pharmaceuticals, but there are only a few examples where regulation of gene cluster expression has been correlated with ecological and physiological relevance for the producer. Here, signals, mediators,... Read More