Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824

Ehsaan, Muhammad; Kuit, Wouter; Zhang, Ying; Cartman, Stephen T.; Heap, John T.; Winzer, Klaus; Minton, Nigel P.

doi:10.1186/s13068-015-0410-0

All Outputs (5)

A genetic assay for gene essentiality in Clostridium (2016)
Journal Article

Essential genes of pathogens are potential therapeutic targets, but are difficult to verify. Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium d... Read More about A genetic assay for gene essentiality in Clostridium.

Insights into CO2 fixation pathway of Clostridium autoethanogenumby targeted mutagenesis (2016)
Journal Article

The future sustainable production of chemicals and fuels from nonpetrochemical resources and reduction of greenhouse gas emissions are two of the greatest societal challenges. Gas fermentation, which utilizes the ability of acetogenic bacteria such a... Read More about Insights into CO2 fixation pathway of Clostridium autoethanogenumby targeted mutagenesis.

Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824 (2016)
Journal Article

Background: The use of fossil fuels is no longer tenable. Not only are they a finite resource, their use is damaging the environment through pollution and global warming. Alternative, environmentally friendly, renewable sources of chemicals and fuels... Read More about Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824.

Dendrimer mediated clustering of bacteria: improved aggregation and evaluation of bacterial response and viability (2016)
Journal Article

Here, we evaluate how cationic gallic acid-triethylene glycol (GATG) dendrimers interact with bacteria and their potential to develop new antimicrobials. We demonstrate that GATG dendrimers functionalised with primary amines in their periphery can in... Read More about Dendrimer mediated clustering of bacteria: improved aggregation and evaluation of bacterial response and viability.

Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824 (2016)
Journal Article

Background Clostridium acetobutylicum represents a paradigm chassis for the industrial production of the biofuel biobutanol and a focus for metabolic engineering. We have previously developed procedures for the creation of in-frame, marker-less de... Read More about Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824.