All Outputs (34)

Extended 2D myotube culture recapitulates postnatal fibre type plasticity (2015)
Journal Article
Sebastian, S., Goulding, L., Kuchipudi, S. V., & Chang, K.-C. (in press). Extended 2D myotube culture recapitulates postnatal fibre type plasticity. BMC Cell Biology, 16(1), https://doi.org/10.1186/s12860-015-0069-1

Background: The traditional problems of performing skeletal muscle cell cultures derived from mammalian or avian species are limited myotube differentiation, and transient myotube persistence which greatly restricts the ability of myotubes to undergo... Read More about Extended 2D myotube culture recapitulates postnatal fibre type plasticity.

PA-X is a virulence factor in avian H9N2 influenza virus (2015)
Journal Article
Gao, H., Liu, J., Kong, W., Sun, H., Pu, J., Chang, K.-C., …Wang, J. (2015). PA-X is a virulence factor in avian H9N2 influenza virus. Journal of General Virology, 96(9), https://doi.org/10.1099/jgv.0.000232

H9N2 influenza viruses have been circulating worldwide in multiple avian species, and regularly infect pigs and humans. Recently, a novel protein, PA-X, produced from the PA gene by ribosomal frameshifting, was demonstrated to be an antivirulence fac... Read More about PA-X is a virulence factor in avian H9N2 influenza virus.

Twenty amino acids at the C-terminus of PA-X are associated with increased influenza A virus replication and pathogenicity (2015)
Journal Article
Gao, H., Sun, Y., Liu, X., Sun, H., Hu, J., Wang, J., …He, Q. (2015). Twenty amino acids at the C-terminus of PA-X are associated with increased influenza A virus replication and pathogenicity. Journal of General Virology, 96(8), https://doi.org/10.1099/vir.0.000143

The PA-X protein, arising from ribosomal frameshift during PA translation, was recently discovered in influenza A virus (IAV). The C-terminal domain ‘X’ of PA-X proteins in IAVs can be classified as full-length (61 aa) or truncated (41 aa). In the ma... Read More about Twenty amino acids at the C-terminus of PA-X are associated with increased influenza A virus replication and pathogenicity.

Investigation into the animal species contents of popular wet pet foods (2015)
Journal Article
Maine, I. R., Atterbury, R. J., & Chang, K.-C. (2015). Investigation into the animal species contents of popular wet pet foods. Acta Veterinaria Scandinavica, 57(1), https://doi.org/10.1186/s13028-015-0097-z

Background: The use of the generic term “meat and animal derivatives” in declared ingredient lists of pet foods in the European Union is virtually universal. In the wake of the 2013 “horse meat scandal” in the human food chain, we examined the presen... Read More about Investigation into the animal species contents of popular wet pet foods.

DNA microarray global gene expression analysis of influenza virus-infected chicken and duck cells (2015)
Journal Article
Kuchipudi, S., Chang, K.-C., & Dunham, S. (2015). DNA microarray global gene expression analysis of influenza virus-infected chicken and duck cells. Genomics Data, 4, 60-64. https://doi.org/10.1016/j.gdata.2015.03.004

The data described in this article pertain to the article by Kuchipudi et al. (2014) titled “Highly Pathogenic Avian Influenza Virus Infection in Chickens But Not Ducks Is Associated with Elevated Host Immune and Pro-inflammatory Responses” [1]. Whil... Read More about DNA microarray global gene expression analysis of influenza virus-infected chicken and duck cells.

The contribution of PA-X to the virulence of pandemic 2009 H1N1 and highly pathogenic H5N1 avian influenza viruses (2015)
Journal Article
Gao, H., Sun, Y., Hu, J., Qi, L., Wang, J., Xiong, X., …Liu, J. (in press). The contribution of PA-X to the virulence of pandemic 2009 H1N1 and highly pathogenic H5N1 avian influenza viruses. Scientific Reports, 5(1), https://doi.org/10.1038/srep08262

PA-X is a novel protein encoded by PA mRNA and is found to decrease the pathogenicity of pandemic 1918 H1N1 virus in mice. However, the importance of PA-X proteins in current epidemiologically important influenza A virus strains is not known. In this... Read More about The contribution of PA-X to the virulence of pandemic 2009 H1N1 and highly pathogenic H5N1 avian influenza viruses.

Chicken and duck myotubes are highly susceptible and permissive to influenza virus infection (2014)
Journal Article
Baquero-Perez, B., Kuchipudi, S. V., Ho, J., Sebastian, S., Puranik, A., Howard, W., …Chang, K.-C. (2015). Chicken and duck myotubes are highly susceptible and permissive to influenza virus infection. Journal of Virology, 89(5), https://doi.org/10.1128/JVI.03421-14

Skeletal muscle, at 30 to 40% of body mass, is the most abundant soft tissue in the body. Besides its primary function in movement and posture, skeletal muscle is a significant innate immune organ with the capacity to produce cytokines and chemokines... Read More about Chicken and duck myotubes are highly susceptible and permissive to influenza virus infection.

Highly pathogenic avian influenza virus infection in chickens but not ducks is associated with elevated host immune and pro-inflammatory responses (2014)
Journal Article
Kuchipudi, S. V., Tellabati, M., Sebastian, S., Londt, B. Z., Jansen, C., Vervelde, L., …Chang, K.-C. (2014). Highly pathogenic avian influenza virus infection in chickens but not ducks is associated with elevated host immune and pro-inflammatory responses. Veterinary Research, 45(1), Article 118. https://doi.org/10.1186/s13567-014-0118-3

© 2014 Kuchipudi et al.; licensee BioMed Central Ltd. Highly pathogenic avian influenza (HPAI) H5N1 viruses cause severe infection in chickens at near complete mortality, but corresponding infection in ducks is typically mild or asymptomatic. To unde... Read More about Highly pathogenic avian influenza virus infection in chickens but not ducks is associated with elevated host immune and pro-inflammatory responses.

High basal expression of interferon-stimulated genes in human bronchial epithelial (BEAS-2B) cells contributes to influenza A virus resistance (2014)
Journal Article
Seng, L.-G., Daly, J. M., Chang, K.-C., & Kuchipudi, S. V. (2014). High basal expression of interferon-stimulated genes in human bronchial epithelial (BEAS-2B) cells contributes to influenza A virus resistance. PLoS ONE, 9, Article e109023. https://doi.org/10.1371/journal.pone.0109023

Respiratory epithelial cells play a key role in influenza A virus (IAV) pathogenesis and host innate response. Transformed human respiratory cell lines are widely used in the study of IAV−host interactions due to their relative convenience, and inher... Read More about High basal expression of interferon-stimulated genes in human bronchial epithelial (BEAS-2B) cells contributes to influenza A virus resistance.

Influenza A Virus Acquires Enhanced Pathogenicity and Transmissibility after Serial Passages in Swine (2014)
Journal Article
Wei, K., Sun, H., Sun, Z., Sun, Y., Kong, W., Pu, J., …Liu, J. (2014). Influenza A Virus Acquires Enhanced Pathogenicity and Transmissibility after Serial Passages in Swine. Journal of Virology, 88(20), 11981-11994. https://doi.org/10.1128/jvi.01679-14

Genetic and phylogenetic analyses suggest that the pandemic H1N1/2009 virus was derived from well-established swine influenza lineages; however, there is no convincing evidence that the pandemic virus was generated from a direct precursor in pigs. Fu... Read More about Influenza A Virus Acquires Enhanced Pathogenicity and Transmissibility after Serial Passages in Swine.

Naturally Occurring Mutations in the PA Gene Are Key Contributors to Increased Virulence of Pandemic H1N1/09 Influenza Virus in Mice (2014)
Journal Article
Sun, Y., Xu, Q., Shen, Y., Liu, L., Wei, K., Sun, H., …Liu, J. (2014). Naturally Occurring Mutations in the PA Gene Are Key Contributors to Increased Virulence of Pandemic H1N1/09 Influenza Virus in Mice. Journal of Virology, 88(8), 4600-4604. https://doi.org/10.1128/jvi.03158-13

We examined the molecular basis of virulence of pandemic H1N1/09 influenza viruses by reverse genetics based on two H1N1/09 virus isolates (A/California/04/2009 [CA04] and A/swine/Shandong/731/2009 [SD731]) with contrasting pathogenicities in mice. W... Read More about Naturally Occurring Mutations in the PA Gene Are Key Contributors to Increased Virulence of Pandemic H1N1/09 Influenza Virus in Mice.

Comparative virus replication and host innate responses in human cells infected with three prevalent clades (2.3.4, 2.3.2, and 7) of highly pathogenic avian influenza H5N1 viruses (2013)
Journal Article
Sun, H., Sun, Y., Pu, J., Zhang, Y., Zhu, Q., Li, J., …Liu, J. (2014). Comparative virus replication and host innate responses in human cells infected with three prevalent clades (2.3.4, 2.3.2, and 7) of highly pathogenic avian influenza H5N1 viruses. Journal of Virology, 88(1), https://doi.org/10.1128/JVI.02510-13

Highly pathogenic avian influenza H5N1 virus clades 2.3.4, 2.3.2, and 7 are the dominant cocirculating H5N1 viruses in poultry in China. However, humans appear to be clinically susceptible mostly to the 2.3.4 virus clade. Here, we demonstrated that A... Read More about Comparative virus replication and host innate responses in human cells infected with three prevalent clades (2.3.4, 2.3.2, and 7) of highly pathogenic avian influenza H5N1 viruses.

Mammalian innate resistance to highly pathogenic avian influenza H5N1 virus infection is mediated through reduced proinflammation and infectious virus release (2012)
Journal Article
Nelli, R. K., Dunham, S. P., Kuchipudi, S. V., White, G. A., Baquero-Perez, B., Pengxiang, C., …Chang, K. (2012). Mammalian innate resistance to highly pathogenic avian influenza H5N1 virus infection is mediated through reduced proinflammation and infectious virus release. Journal of Virology, 86(17), https://doi.org/10.1128/JVI.00244-12

Respiratory epithelial cells and macrophages are the key innate immune cells that play an important role in the pathogenesis of influenza A virus infection. We found that these two cell types from both human and pig showed comparable susceptibilities... Read More about Mammalian innate resistance to highly pathogenic avian influenza H5N1 virus infection is mediated through reduced proinflammation and infectious virus release.

18S rRNA is a reliable normalisation gene for real time PCR based on influenza virus infected cells
Journal Article
Kuchipudi, S. V., Tellabati, M., Nelli, R. K., White, G. A., Baquero Perez, B., Sebastian, S., …Chang, K. 18S rRNA is a reliable normalisation gene for real time PCR based on influenza virus infected cells. Virology Journal, 9(230), https://doi.org/10.1186/1743-422X-9-230

Background: One requisite of quantitative reverse transcription PCR (qRT-PCR) is to normalise the data with an
internal reference gene that is invariant regardless of treatment, such as virus infection. Several studies have found
variability in the... Read More about 18S rRNA is a reliable normalisation gene for real time PCR based on influenza virus infected cells.