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Chicken and duck myotubes are highly susceptible and permissive to influenza virus infection

Baquero-Perez, Belinda; Kuchipudi, Suresh V.; Ho, Jemima; Sebastian, Sujith; Puranik, Anita; Howard, Wendy; Brookes, Sharon M.; Brown, Ian H.; Chang, Kin-Chow

Authors

Belinda Baquero-Perez

Suresh V. Kuchipudi

Jemima Ho

Sujith Sebastian

Anita Puranik

Wendy Howard

Sharon M. Brookes

Ian H. Brown

KIN-CHOW CHANG kin-chow.chang@nottingham.ac.uk
Professor of Veterinary Molecular Medicine



Abstract

Skeletal muscle, at 30 to 40% of body mass, is the most abundant soft tissue in the body. Besides its primary function in movement and posture, skeletal muscle is a significant innate immune organ with the capacity to produce cytokines and chemokines and respond to proinflammatory cytokines. Little is known about the role of skeletal muscle during systemic influenza A virus infection in any host and particularly avian species. Here we used primary chicken and duck multinucleated myotubes to examine their susceptibility and innate immune response to influenza virus infections. Both chicken and duck myotubes expressed avian and human sialic acid receptors and were readily susceptible to low-pathogenicity (H2N3 A/mallard duck/England/7277/06) and high-pathogenicity (H5N1 A/turkey/England/50-92/91 and H5N1 A/turkey/Turkey/1/05) avian and human H1N1 (A/USSR/77) influenza viruses. Both avian host species produced comparable levels of progeny H5N1 A/turkey/Turkey/1/05 virus.Notably, the rapid accumulation of viral nucleoprotein and matrix (M) gene RNA in chicken and duck myotubes was accompanied by extensive cytopathic damage with marked myotube apoptosis (widespread microscopic blebs, caspase 3/7 activation, and annexin V binding at the plasma membrane). Infected chicken myotubes produced significantly higher levels of proinflammatory cytokines than did the corresponding duck cells. Additionally, in chicken myotubes infected with H5N1 viruses, the induction of interferon beta (IFN-beta) and IFN-inducible genes, including the melanoma differentiation-associated protein 5 (MDA-5) gene, was relatively weak compared to infection with the corresponding H2N3 virus. Our findings highlight that avian skeletal muscle fibers are capable of productive influenza virus replication and are a potential tissue source of infection.

Journal Article Type Article
Publication Date Mar 1, 2015
Journal Journal of Virology
Print ISSN 0022-538X
Electronic ISSN 0022-538X
Publisher American Society for Microbiology
Peer Reviewed Peer Reviewed
Volume 89
Issue 5
APA6 Citation Baquero-Perez, B., Kuchipudi, S. V., Ho, J., Sebastian, S., Puranik, A., Howard, W., …Chang, K. (2015). Chicken and duck myotubes are highly susceptible and permissive to influenza virus infection. Journal of Virology, 89(5), https://doi.org/10.1128/JVI.03421-14
DOI https://doi.org/10.1128/JVI.03421-14
Publisher URL http://jvi.asm.org/content/89/5/2494
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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J. Virol.-2015-Baquero-Perez-2494-506.pdf (4.3 Mb)
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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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