All Outputs (34)

Sub-100 nm resolution microscopy based on proximity projection grating scheme (2015)
Journal Article
Hu, F., Somekh, M. G., Albutt, D. J., Webb, K., Moradi, E., & See, C. W. (2015). Sub-100 nm resolution microscopy based on proximity projection grating scheme. Scientific Reports, 5, Article 8589. https://doi.org/10.1038/srep08589

Structured illumination microscopy (SIM) has been widely used in life science imaging applications. The maximum resolution improvement of SIM, compared to conventional bright field system is a factor of 2. Here we present an approach to structured il... Read More about Sub-100 nm resolution microscopy based on proximity projection grating scheme.

Condenser-free contrast methods for transmitted-light microscopy (2014)
Journal Article
Webb, K. F. (2014). Condenser-free contrast methods for transmitted-light microscopy. Journal of Microscopy, 257(1), 8-22. https://doi.org/10.1111/jmi.12181

Phase contrast microscopy allows the study of highly transparent yet detail-rich specimens by producing intensity contrast from phase objects within the sample. Presented here is a generalized phase contrast illumination schema in which condenser opt... Read More about Condenser-free contrast methods for transmitted-light microscopy.

Sound of nano (2013)
Presentation / Conference Contribution
Aylott, J., Clark, M., Marques, L., Perez-Cota, F., Smith, R. J., & Webb, K. F. (2013). Sound of nano. Proceedings of SPIE, 8923, Article 892325. https://doi.org/10.1117/12.2035176

Ultrasound is widely used for imaging, measurement and diagnostics in the MHz region and is perhaps most familiar as a medical or non-destructive imaging or measurement tool. In the MHz frequency range the wavelength is typically measured in microns... Read More about Sound of nano.

Identification of a nonselective cation channel in isolated lens fiber cells that is activated by cell shrinkage (2012)
Journal Article
Gunning, S. J., Chung, K. K., Donaldson, P. J., & Webb, K. F. (2012). Identification of a nonselective cation channel in isolated lens fiber cells that is activated by cell shrinkage. American Journal of Physiology - Cell Physiology, 303(12), C1252-C1259. https://doi.org/10.1152/ajpcell.00237.2012

The initiation of lens cataract has long been associated with the development of a membrane "leak" in lens fiber cells that depolarizes the lens intracellular potential and elevates intracellular Na+ and Ca2+ concentrations. It has been proposed that... Read More about Identification of a nonselective cation channel in isolated lens fiber cells that is activated by cell shrinkage.

Multimodal imaging of fluid transport in living epithelial sheets (2011)
Presentation / Conference Contribution
Webb, K. F., Zhang, J., & Somekh, M. (2011). Multimodal imaging of fluid transport in living epithelial sheets. In 2011 Functional Optical Imaging. https://doi.org/10.1109/FOI.2011.6154825

Detailed knowledge on the biophysical mechanisms and local intercompartmental dynamics by which fluid and solute transport is achieved and regulated at the cellular and subcellular level is lacking. In this paper, cultures of immortalised human retin... Read More about Multimodal imaging of fluid transport in living epithelial sheets.

Functional gap junctions accumulate at the immunological synapse and contribute to T cell activation (2011)
Journal Article
Mendoza-Naranjo, A., Bouma, G., Pereda, C., Ramiŕez, M., Webb, K. F., Tittarelli, A., …Salazar-Onfray, F. (2011). Functional gap junctions accumulate at the immunological synapse and contribute to T cell activation. Journal of Immunology, 187(6), 3121-3132. https://doi.org/10.4049/jimmunol.1100378

Gap junction (GJ) mediates intercellular communication through linked hemichannels from each of two adjacent cells. Using human and mouse models, we show that connexin 43 (Cx43), the main GJ protein in the immune system, was recruited to the immunolo... Read More about Functional gap junctions accumulate at the immunological synapse and contribute to T cell activation.

Ionic Permeability and Currents in the Lens (2010)
Book Chapter
Donaldson, P. J., & Webb, K. F. (2010). Ionic Permeability and Currents in the Lens. In Encyclopedia of the Eye (477-486). https://doi.org/10.1016/B978-0-12-374203-2.00032-4

© 2010 Elsevier Ltd. All rights reserved. It has been proposed that in the absence of a blood supply the lens operates an internal microcirculation system. In this system spatially distinct differences in the membrane transport properties of the lens... Read More about Ionic Permeability and Currents in the Lens.

Whole-cell patch clamping of isolated fiber cells confirms that spatially distinct Cl- influx and efflux pathways exist in the cortex of the rat lens (2009)
Journal Article
Webb, K. F., & Donaldson, P. J. (2009). Whole-cell patch clamping of isolated fiber cells confirms that spatially distinct Cl- influx and efflux pathways exist in the cortex of the rat lens. Investigative Ophthalmology & Visual Science, 50(8), 3808-3818. https://doi.org/10.1167/iovs.08-2680

PURPOSE. To test the hypothesis that lens fiber cells use different combinations of transport proteins to mediate Cl influx and efflux in order to regulate their steady state volume.

METHODS. Cells were isolated from rat lenses by enzymatic disso... Read More about Whole-cell patch clamping of isolated fiber cells confirms that spatially distinct Cl- influx and efflux pathways exist in the cortex of the rat lens.

Regulation of lens volume: Implications for lens transparency (2009)
Journal Article
Donaldson, P. J., Chee, K. S. N., Lim, J. C., & Webb, K. F. (2009). Regulation of lens volume: Implications for lens transparency. Experimental Eye Research, 88(2), 144-150. https://doi.org/10.1016/j.exer.2008.05.011

Lens transparency is critically dependent on the maintenance of an ordered tissue architecture, and disruption of this order leads to light scatter and eventually lens cataract. Hence the volume of the fiber cells that make up the bulk of the lens ne... Read More about Regulation of lens volume: Implications for lens transparency.

Molecular and functional mapping of regional differences in P2Y receptor expression in the rat lens (2008)
Journal Article
Hu, R. G., Suzuki-Kerr, H., Webb, K. F., Rhodes, J. D., Collison, D. J., Duncan, G., & Donaldson, P. J. (2008). Molecular and functional mapping of regional differences in P2Y receptor expression in the rat lens. Experimental Eye Research, 87(2), 137-146. https://doi.org/10.1016/j.exer.2008.05.008

Extracellular ATP has been shown to mobilize intracellular Ca2+ in cultured ovine lens epithelial cells and in human lens epithelium, suggesting a role for purines in the modulation of lens transparency. In this study, we characterized the expression... Read More about Molecular and functional mapping of regional differences in P2Y receptor expression in the rat lens.

Differentiation-dependent changes in the membrane properties of fiber cells isolated from the rat lens (2008)
Journal Article
Webb, K. F., & Donaldson, P. J. (2008). Differentiation-dependent changes in the membrane properties of fiber cells isolated from the rat lens. American Journal of Physiology - Cell Physiology, 294(5), C1133-C1145. https://doi.org/10.1152/ajpcell.00315.2007

Impedance measurements in whole lenses showed that lens fiber cells possess different permeability properties to the epithelial cells from which they differentiate. To confirm these observations at the cellular level, we analyzed the membrane propert... Read More about Differentiation-dependent changes in the membrane properties of fiber cells isolated from the rat lens.

Multiphoton imaging of chick retinal development in relation to gap junctional communication (2007)
Journal Article
Becker, D. L., Webb, K. F., Thrasivoulou, C., Lin, C. C., Nadershahi, R., Tsakiri, N., & Cook, J. E. (2007). Multiphoton imaging of chick retinal development in relation to gap junctional communication. Journal of Physiology, 585(3), 711-719. https://doi.org/10.1113/jphysiol.2007.138776

Neural progenitor cells in the developing retina extend processes that stretch from the basal vitread surface to the apical ventricular surface. During the cell cycle, the nucleus undergoes interkinetic nuclear migration (INM), moving in a vitread di... Read More about Multiphoton imaging of chick retinal development in relation to gap junctional communication.

Spatially Distinct Cl– Influx and Efflux Pathways Interact to Maintain Lens Volume and Transparency (2005)
Journal Article
Donaldson, P., Chee, K., Webb, K., & Kistler, J. (2005). Spatially Distinct Cl– Influx and Efflux Pathways Interact to Maintain Lens Volume and Transparency. Investigative Ophthalmology & Visual Science, 46(13), Article 1129

Cl− Influx into Rat Cortical Lens Fiber Cells Is Mediated by a Cl− Conductance That Is Not ClC-2 or -3 (2004)
Journal Article
Webb, K. F., Merriman-Smith, B. R., Stobie, J. K., Kistler, J., & Donaldson, P. J. (2004). Cl− Influx into Rat Cortical Lens Fiber Cells Is Mediated by a Cl− Conductance That Is Not ClC-2 or -3. Investigative Ophthalmology & Visual Science, 45(12), 4400-4408. https://doi.org/10.1167/iovs.04-0205

PURPOSE. Exposure of organ-cultured lenses to C1- channel blockers under isotonic conditions induces a localized cortical zone of extracellular space dilations. The purpose of this study was to investigate whether elongated lens fiber cells from this... Read More about Cl− Influx into Rat Cortical Lens Fiber Cells Is Mediated by a Cl− Conductance That Is Not ClC-2 or -3.