All Outputs (34)

Mapping Polyclonal Antibody Responses to Infection Using Next-Generation Phage Display (2023)
Book Chapter

Peptide phage display has historically been used to epitope map monoclonal antibodies. More recently, by coupling this method with next-generation sequencing (so-called next-generation phage display, NGPD) to mass screen peptide binding events, the m... Read More about Mapping Polyclonal Antibody Responses to Infection Using Next-Generation Phage Display.

Recombinant ovine prion protein can be mutated at position 136 to improve its efficacy as an inhibitor of prion propagation (2023)
Journal Article

Prion diseases are progressive neurodegenerative disorders with no effective therapeutics. The central event leading to the pathology in the diseases is the conversion of PrPC into PrPSc and its accumulation in the central nervous system. Previous st... Read More about Recombinant ovine prion protein can be mutated at position 136 to improve its efficacy as an inhibitor of prion propagation.

Therapeutic Phage Display-Derived Single-Domain Antibodies for Pandemic Preparedness (2023)
Journal Article

Driven by necessity, the COVID-19 pandemic caused by SARS-CoV-2 has accelerated the development and implementation of new vaccine platforms and other viral therapeutics. Among these is the therapeutic use of antibodies including single-domain antibod... Read More about Therapeutic Phage Display-Derived Single-Domain Antibodies for Pandemic Preparedness.

Next-Generation Phage Display to Identify Peptide Ligands of Deubiquitinases (2022)
Book Chapter

Phage display (PD) is a powerful method and has been extensively used to generate monoclonal antibodies and identify epitopes, mimotopes, and protein interactions. More recently, the combination of next-generation sequencing (NGS) with PD (NGPD) has... Read More about Next-Generation Phage Display to Identify Peptide Ligands of Deubiquitinases.

A Simple Whole-Plasmid PCR Method to Construct High-Diversity Synthetic Phage Display Libraries (2022)
Journal Article

Phage display technology utilises peptide and antibody libraries with very high diversities to select ligands with specific binding properties. The production of such libraries can be labour intensive and technically challenging and whilst there are... Read More about A Simple Whole-Plasmid PCR Method to Construct High-Diversity Synthetic Phage Display Libraries.

BSE can propagate in sheep co-infected or pre-infected with scrapie (2021)
Journal Article

To understand the possible role of mixed-prion infections in disease presentation, the current study reports the co-infection of sheep with bovine spongiform encephalopathy (BSE) and scrapie. The bovine BSE agent was inoculated subcutaneously into sh... Read More about BSE can propagate in sheep co-infected or pre-infected with scrapie.

The ‘dark matter’ of ubiquitin-mediated processes: opportunities and challenges in the identification of ubiquitin-binding domains (2019)
Journal Article

Ubiquitin modifications of target proteins act to localise, direct and specify a diverse range of cellular processes, many of which are biomedically relevant. To allow this diversity, ubiquitin modifications exhibit remarkable complexity, determined... Read More about The ‘dark matter’ of ubiquitin-mediated processes: opportunities and challenges in the identification of ubiquitin-binding domains.

Advances in the Production and Batch Reformatting of Phage Antibody Libraries (2019)
Journal Article

Phage display antibody libraries have proven an invaluable resource for the isolation of diagnostic and potentially therapeutic antibodies, the latter usually being antibody fragments converted into IgG formats. Recent advances in the production of h... Read More about Advances in the Production and Batch Reformatting of Phage Antibody Libraries.

BSE infectivity survives burial for five years with only limited spread (2019)
Journal Article

© 2019, The Author(s). The carcasses of animals infected with bovine spongiform encephalopathy (BSE), scrapie or chronic wasting disease (CWD) that remain in the environment (exposed or buried) may continue to act as reservoirs of infectivity. We co... Read More about BSE infectivity survives burial for five years with only limited spread.

Discovery of peptide ligands targeting a specific ubiquitin-like domain– binding site in the deubiquitinase USP11 (2018)
Journal Article

© 2019 Spiliotopoulos et al. Published by The American Society for Biochemistry and Molecular Biology, Inc. Ubiquitin-specific proteases (USPs) reverse ubiquitination and regulate virtually all cellular processes. Defined noncatalytic domains in USP... Read More about Discovery of peptide ligands targeting a specific ubiquitin-like domain– binding site in the deubiquitinase USP11.

Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of the great crested newt (Triturus cristatus) (2018)
Journal Article

Environmental DNA (eDNA) analysis is a rapid, cost-effective, non-invasive biodiversity monitoring tool which utilises DNA left behind in the environment by organisms for species detection. The method is used as a species-specific survey tool for rar... Read More about Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of the great crested newt (Triturus cristatus).

The chronic stages of bovine Fasciola hepatica are dominated by CD4 T-Cell exhaustion (2017)
Journal Article

Fasciola hepatica infection of ruminants leads to non-resolving chronic infection, as patency develops, there is switching to a TGF-? and IL-10 led response. Here, we explore the responses of CD4 T-cells within the major draining lymph nodes. We foun... Read More about The chronic stages of bovine Fasciola hepatica are dominated by CD4 T-Cell exhaustion.

The detection of great crested newts year round via environmental DNA analysis (2017)
Journal Article

Objective Analysis of environmental DNA (eDNA) is a method that has been used for the detection of various species within water bodies. The great crested newt (Triturus cristatus) has a short eDNA survey season (mid-April to June). Here we investi... Read More about The detection of great crested newts year round via environmental DNA analysis.

An in vitro model for assessing effective scrapie decontamination (2017)
Journal Article

Scrapie infectivity enters the environment via a multiplicity of routes from infected animals. Environmentally associated scrapie persists on farms when infected animals have been removed and is particularly resistant to disinfection. Infectivity wit... Read More about An in vitro model for assessing effective scrapie decontamination.

How is Europe positioned for a re-emergence of Schmallenberg virus? (2017)
Journal Article

The Schmallenberg virus (SBV) caused a large scale epidemic in Europe from 2011–2013 infecting ruminants and causing fetal deformities after infection of pregnant animals. The main impacts of the virus were financial losses due to animal, meat and se... Read More about How is Europe positioned for a re-emergence of Schmallenberg virus?.

In vitro amplification of H-type atypical bovine spongiform encephalopathy by protein misfolding cyclic amplification (2017)
Journal Article

The in vitro amplification of prions by serial protein misfolding cyclic amplification has been shown to detect PrPSc to levels at least as sensitive as rodent bioassay but in a fraction of the time. Bovine spongiform encephalopathy is a zoonotic pri... Read More about In vitro amplification of H-type atypical bovine spongiform encephalopathy by protein misfolding cyclic amplification.

A Trematode Parasite Derived Growth Factor Binds and Exerts Influences on Host Immune Functions via Host Cytokine Receptor Complexes (2016)
Journal Article

© 2016 Sulaiman et al. The trematode Fasciola hepatica is responsible for chronic zoonotic infection globally. Despite causing a potent T-helper 2 response, it is believed that potent immunomodulation is responsible for rendering this host reactive n... Read More about A Trematode Parasite Derived Growth Factor Binds and Exerts Influences on Host Immune Functions via Host Cytokine Receptor Complexes.

A sensitive 301V BSE serial PMCA assay (2016)
Journal Article

The prion strain 301V, is a mouse passaged form of bovine spongiform encephalopathy (BSE). It has been used as a model of BSE for more than 20 years, in particular in the investigation of tissue distribution of infectivity, the molecular phenotype an... Read More about A sensitive 301V BSE serial PMCA assay.

Mapping B-cell responses to Salmonella enterica serovars Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals (2016)
Journal Article

Serological surveillance and vaccination are important strategies for controlling infectious diseases of food production animals. However, the compatibility of these strategies is limited by a lack of assays capable of di erentiating infected from va... Read More about Mapping B-cell responses to Salmonella enterica serovars Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals.

Mapping polyclonal antibody responses to bacterial infection using next generation phage display (2016)
Journal Article

Mapping polyclonal antibody responses to infectious diseases to identify individual epitopes has the potential to underpin the development of novel serological assays and vaccines. Here, phage-peptide library panning coupled with screening using next... Read More about Mapping polyclonal antibody responses to bacterial infection using next generation phage display.