All Outputs (147)

Illustrated Dictionary of Parasitology in the Post-Genomic Era (2017)
Book
Elsheikha, H., & Jarroll, E. L. (2017). Illustrated Dictionary of Parasitology in the Post-Genomic Era. Caister Academic Press Limited. https://doi.org/10.21775/9781910190678

This illustrated dictionary provides concise definitions and explanations of parasitology terms and related molecular processes presented in an easy-to-use, A-Z order with particular emphasis on terms that are of relevance to parasite biotechnology a... Read More about Illustrated Dictionary of Parasitology in the Post-Genomic Era.

Profiling of the perturbed metabolomic state of mouse spleen during acute and chronic toxoplasmosis (2017)
Journal Article
Chen, X.-Q., Zhou, C.-X., Elsheikha, H. M., He, S., Hu, G.-X., & Zhu, X.-Q. (2017). Profiling of the perturbed metabolomic state of mouse spleen during acute and chronic toxoplasmosis. Parasites and Vectors, 10(1), Article 339. https://doi.org/10.1186/s13071-017-2282-6

Background

Toxoplasma gondii, a common opportunistic protozoan, is a leading cause of illness and mortality among immunosuppressed individuals and during congenital infections. Current therapeutic strategies for toxoplasmosis are not fully effecti... Read More about Profiling of the perturbed metabolomic state of mouse spleen during acute and chronic toxoplasmosis.

Toxoplasma gondii infection in farmed wild boars (Sus scrofa) in three cities of northeast China (2017)
Journal Article
Bai, M.-J., Zou, Y., Elsheikha, H. M., Ma, J.-G., Zheng, W.-B., Zhao, Q., …Zhu, X.-Q. (2017). Toxoplasma gondii infection in farmed wild boars (Sus scrofa) in three cities of northeast China. Foodborne Pathogens and Disease, 14(7), 379-385. https://doi.org/10.1089/fpd.2016.2260

The apicomplexan protozoan parasite Toxoplasma gondii is a widely distributed etiological agent of foodborne illness. This parasite can cause production losses in livestock and serious disease in humans through consumption of contaminated meat. Pig m... Read More about Toxoplasma gondii infection in farmed wild boars (Sus scrofa) in three cities of northeast China.

Comparative proteomic analysis of virulent and avirulent strains of Toxoplasma gondii reveals strain-specific patterns (2017)
Journal Article
Zhou, D.-H., Wang, Z.-X., Zhou, C.-X., He, S., Elsheikha, H. M., & Zhu, X.-Q. (2017). Comparative proteomic analysis of virulent and avirulent strains of Toxoplasma gondii reveals strain-specific patterns. Oncotarget, 8(46), https://doi.org/10.18632/oncotarget.19077

Research exploring the proteome of Toxoplasma gondii oocysts has gained momentum over the past few years. However, little is known about the oocyst’s protein repertoires that contribute to differential virulence among T. gondii strains. Here, we used... Read More about Comparative proteomic analysis of virulent and avirulent strains of Toxoplasma gondii reveals strain-specific patterns.

Immunization with Toxoplasma gondii GRA17 deletion mutant induces partial protection and survival in challenged mice (2017)
Journal Article
Wang, J., Elsheikha, H. M., Zhu, W., Chen, K., Li, T., Yue, D., …Zhu, X. (2017). Immunization with Toxoplasma gondii GRA17 deletion mutant induces partial protection and survival in challenged mice. Frontiers in Immunology, 8, Article 730. https://doi.org/10.3389/fimmu.2017.00730

Toxoplasmosis remains a world-threatening disease largely because of the lack of a fully effective vaccine. Here, we created a ΔGRA17 mutant by disrupting the virulence factor GRA17 using CRISPR-Cas9 method. Then, we tested whether ΔGRA17 tachyzoites... Read More about Immunization with Toxoplasma gondii GRA17 deletion mutant induces partial protection and survival in challenged mice.

Proteomic differences between developmental stages of Toxoplasma gondii revealed by iTRAQ-based quantitative proteomics (2017)
Journal Article
Wang, Z.-X., Zhou, C.-X., Elsheikha, H. M., He, S., Zhou, D.-H., & Zhu, X.-Q. (in press). Proteomic differences between developmental stages of Toxoplasma gondii revealed by iTRAQ-based quantitative proteomics. Frontiers in Microbiology, 8, https://doi.org/10.3389/fmicb.2017.00985

Toxoplasma gondii has a complex two-host life-cycle between intermediate host and definitive host. Understanding proteomic variations across the life-cycle stages of T. gondii may improve the understanding of molecular adaption mechanism of T. gondii... Read More about Proteomic differences between developmental stages of Toxoplasma gondii revealed by iTRAQ-based quantitative proteomics.

Global miRNA expression profiling of domestic cat livers following acute Toxoplasma gondii infection (2017)
Journal Article
Cong, W., Zhang, X.-X., He, J.-J., Li, F.-C., Elsheikha, H. M., & Zhu, X.-Q. (2017). Global miRNA expression profiling of domestic cat livers following acute Toxoplasma gondii infection. Oncotarget, 8(15), https://doi.org/10.18632/oncotarget.16108

Although microRNAs (miRNAs) play an important role in liver homeostasis, the extent to which they can be altered by Toxoplasma gondii infection is unknown. Here, we utilized small RNA sequencing and bioinformatic analyses to characterize miRNA expres... Read More about Global miRNA expression profiling of domestic cat livers following acute Toxoplasma gondii infection.

De novo transcriptome sequencing and analysis of the juvenile and adult stages of Fasciola gigantica (2017)
Journal Article
Zhang, X.-X., Cong, W., Elsheikha, H. M., Liu, G.-H., Ma, J.-G., Huang, W.-Y., …Zhu, X.-Q. (2017). De novo transcriptome sequencing and analysis of the juvenile and adult stages of Fasciola gigantica. Infection, Genetics and Evolution, 51, 33-40. https://doi.org/10.1016/j.meegid.2017.03.007

Fasciola gigantica is regarded as the major liver fluke causing fasciolosis in livestock in tropical countries. Despite the significant economic and public health impacts of F. gigantica there are few studies on the pathogenesis of this parasite and... Read More about De novo transcriptome sequencing and analysis of the juvenile and adult stages of Fasciola gigantica.

Transcriptomic responses of water buffalo liver to infection with the digenetic fluke Fasciola gigantica (2017)
Journal Article
Zhang, F.-K., Zhang, X.-X., Elsheikha, H. M., He, J.-J., Sheng, Z.-A., Zheng, W.-B., …Zhu, X.-Q. (2017). Transcriptomic responses of water buffalo liver to infection with the digenetic fluke Fasciola gigantica. Parasites and Vectors, 10(1), Article 56. https://doi.org/10.1186/s13071-017-1990-2

Background
Fasciola gigantica, the tropical liver fluke, infects buffaloes in Asian and African countries and causes significant economic losses and poses public health threat in these countries. However, little is known of the transcriptional respo... Read More about Transcriptomic responses of water buffalo liver to infection with the digenetic fluke Fasciola gigantica.

Functional characterization of rhoptry kinome in the virulent toxoplasma gondii RH strain (2017)
Journal Article
Wang, J.-L., Li, T.-T., Elsheikha, H. M., Chen, K., Zhu, W.-N., Yue, D.-M., …Huang, S.-Y. (in press). Functional characterization of rhoptry kinome in the virulent toxoplasma gondii RH strain. Frontiers in Microbiology, 8, Article 84. https://doi.org/10.3389/fmicb.2017.00084

Toxoplasma gondii is an obligatory intracellular apicomplexan protozoan which can infect any warm-blooded animal and causes severe diseases in immunocompromised individuals or infants infected in utero. The survival and success of this parasite requi... Read More about Functional characterization of rhoptry kinome in the virulent toxoplasma gondii RH strain.

Identification of host proteins interacting with Toxoplasma gondii GRA15 (TgGRA15) by yeast two-hybrid system (2017)
Journal Article
Liu, Q., Li, F.-C., Elsheikha, H. M., Sun, M.-M., & Zhu, X.-Q. (2017). Identification of host proteins interacting with Toxoplasma gondii GRA15 (TgGRA15) by yeast two-hybrid system. Parasites and Vectors, 10(1), https://doi.org/10.1186/s13071-016-1943-1

Background

Toxoplasma gondii, an obligate intracellular protozoan parasite, possesses the remarkable ability to co-opt host cell machinery in order to maintain its intracellular survival. This parasite can modulate signaling pathways of its host t... Read More about Identification of host proteins interacting with Toxoplasma gondii GRA15 (TgGRA15) by yeast two-hybrid system.

STAT2 is a pervasive cytokine regulator due to its inhibition of STAT1 in multiple signaling pathways (2016)
Journal Article
Ho, J., Pelzel, C., Begitt, A., Mee, M., Elsheikha, H. M., Scott, D. J., & Vinkemeier, U. (2016). STAT2 is a pervasive cytokine regulator due to its inhibition of STAT1 in multiple signaling pathways. PLoS Biology, 14(10), 1-27. https://doi.org/10.1371/journal.pbio.2000117

STAT2 is the quintessential transcription factor for type 1 interferons (IFNs), where it functions as a heterodimer with STAT1. However, the human and murine STAT2-deficient phenotypes suggest important additional and currently unidentified type 1 IF... Read More about STAT2 is a pervasive cytokine regulator due to its inhibition of STAT1 in multiple signaling pathways.

Acanthamoeba castellanii : A new high-throughput method for drug screening in vitro (2016)
Journal Article
Ortega-Rivas, A., Padrón, J. M., Valladares, B., & Elsheikha, H. M. (2016). Acanthamoeba castellanii : A new high-throughput method for drug screening in vitro. Acta Tropica, 164, 95-99. https://doi.org/10.1016/j.actatropica.2016.09.006

Despite significant public health impact, there is no specific antiprotozoal therapy for prevention and treatment of Acanthamoeba castellanii infection. There is a need for new and efficient anti-Acanthamoeba drugs that are less toxic and can reduce... Read More about Acanthamoeba castellanii : A new high-throughput method for drug screening in vitro.

Transcriptomic analysis of mouse liver reveals a potential hepato-enteric pathogenic mechanism in acute Toxoplasma gondii infection (2016)
Journal Article
He, J., Ma, J., Elsheikha, H. M., Song, H., Huang, S., & Zhu, X. (2016). Transcriptomic analysis of mouse liver reveals a potential hepato-enteric pathogenic mechanism in acute Toxoplasma gondii infection. Parasites and Vectors, 9(1), Article 427. https://doi.org/10.1186/s13071-016-1716-x

Background

Toxoplasma gondii is a worldwide spread pathogen which can infect all tissues of its host. The transcriptomic responses of infected brain and spleen have been reported. However, our knowledge of the global transcriptomic change in infec... Read More about Transcriptomic analysis of mouse liver reveals a potential hepato-enteric pathogenic mechanism in acute Toxoplasma gondii infection.

Global iTRAQ-based proteomic profiling of Toxoplasma gondii oocysts during sporulation (2016)
Journal Article
Zhou, C.-X., Zhu, X.-Q., Elsheikha, H. M., He, S., Li, Q., Zhou, D.-H., & Suo, X. (2016). Global iTRAQ-based proteomic profiling of Toxoplasma gondii oocysts during sporulation. Journal of Proteomics, 148, 12-19. https://doi.org/10.1016/j.jprot.2016.07.010

Toxoplasma gondii is a medically and economically important protozoan parasite. However, the molecular mechanisms of its sporulation remain largely unknown. Here, we applied iTRAQ coupled with 2D LC–MS/MS proteomic analysis to investigate the proteom... Read More about Global iTRAQ-based proteomic profiling of Toxoplasma gondii oocysts during sporulation.

Updates on feline aelurostrongylosis and research priorities for the next decade (2016)
Journal Article
Elsheikha, H. M., Schnyder, M., Traversa, D., Di Cesare, A., Wright, I., & Lacher, D. W. (2016). Updates on feline aelurostrongylosis and research priorities for the next decade. Parasites and Vectors, 9, Article 389. https://doi.org/10.1186/s13071-016-1671-6

Feline aelurostrongylosis, caused by the metastrongyloid nematode Aelurostrongylus abstrusus, is an important gastropod-borne parasitic lung disease in cats. Infection with A. abstrusus is widespread globally, but the increasing awareness of this par... Read More about Updates on feline aelurostrongylosis and research priorities for the next decade.

Tracking amino acid’s uptake into the protozoan Acanthamoeba castellanii by stable-isotope labelling and Raman spectral imaging (2016)
Presentation / Conference Contribution
Naemat, A., Elsheikha, H. M., & Notingher, I. (2016). Tracking amino acid’s uptake into the protozoan Acanthamoeba castellanii by stable-isotope labelling and Raman spectral imaging. Proceedings of SPIE, 9887, Article 988715. https://doi.org/10.1117/12.2227070

The capacity of pathogens to acquire nutrients from their host cells is one of the most fundamental aspects of infection biology. Hence, measuring the patterns of nutrients’ uptake by pathogens is essential for understanding the interactions of patho... Read More about Tracking amino acid’s uptake into the protozoan Acanthamoeba castellanii by stable-isotope labelling and Raman spectral imaging.

Proteomic profiling of mouse liver following acute Toxoplasma gondii infection (2016)
Journal Article
He, J.-J., Ma, J., Elsheikha, H. M., Song, H.-Q., Zhou, D.-H., & Zhu, X.-Q. (2016). Proteomic profiling of mouse liver following acute Toxoplasma gondii infection. PLoS ONE, 11(3), 1-15. https://doi.org/10.1371/journal.pone.0152022

© 2016 He et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credite... Read More about Proteomic profiling of mouse liver following acute Toxoplasma gondii infection.

Tracing amino acid exchange during host-pathogen interaction by combined stable-isotope time-resolved Raman spectral imaging (2016)
Journal Article
Naemat, A., Elsheikha, H. M., Boiter, R., & Notingher, I. (2016). Tracing amino acid exchange during host-pathogen interaction by combined stable-isotope time-resolved Raman spectral imaging. Scientific Reports, 6, Article 20811. https://doi.org/10.1038/srep20811

This study investigates the temporal and spatial interchange of the aromatic amino acid phenylalanine (Phe) between human retinal pigment epithelial cell line (ARPE-19) and tachyzoites of the apicomplexan protozoan parasite Toxoplasma gondii (T. gond... Read More about Tracing amino acid exchange during host-pathogen interaction by combined stable-isotope time-resolved Raman spectral imaging.

Metabolomic Profiling of Mice Serum during Toxoplasmosis Progression Using Liquid Chromatography-Mass Spectrometry (2016)
Journal Article
Zhou, C., Zhou, D. -., Elsheikha, H. M., Zhao, Y., Suo, X., & Zhu, X. (2016). Metabolomic Profiling of Mice Serum during Toxoplasmosis Progression Using Liquid Chromatography-Mass Spectrometry. Scientific Reports, 6, Article 19557. https://doi.org/10.1038/srep19557

Better understanding of the molecular changes associated with disease is essential for identifying new routes to improved therapeutics and diagnostic tests. The aim of this study was to investigate the dynamic changes in the metabolic profile of mous... Read More about Metabolomic Profiling of Mice Serum during Toxoplasmosis Progression Using Liquid Chromatography-Mass Spectrometry.