Dong-Hui Zhou
Comparative proteomic analysis of virulent and avirulent strains of Toxoplasma gondii reveals strain-specific patterns
Zhou, Dong-Hui; Wang, Ze-Xiang; Zhou, Chun-Xue; He, Shuai; Elsheikha, Hany M.; Zhu, Xing-Quan
Authors
Ze-Xiang Wang
Chun-Xue Zhou
Shuai He
Professor HANY ELSHEIKHA hany.elsheikha@nottingham.ac.uk
Professor of Interdisciplinary Parasitology
Xing-Quan Zhu
Abstract
Research exploring the proteome of Toxoplasma gondii oocysts has gained momentum over the past few years. However, little is known about the oocyst’s protein repertoires that contribute to differential virulence among T. gondii strains. Here, we used isobaric tag for relative and absolute quantitation-based proteomic analysis of oocysts of two T. gondii strains exhibiting the virulent PYS (ToxoDB#9) phenotype versus the less virulent PRU (Type II, ToxoDB #1) phenotype. Our aim was to determine protein expression patterns that contribute to the virulence of a particular phenotype. A total of 2,551 proteins were identified, of which 374 were differentially expressed proteins (DEPs) (|log2 fold change| ≥ 0.58 and P < 0.05). DEPs included 192 increased and 182 decreased proteins. Gene Ontology and KEGG pathway analyses revealed a large number of DEPs enriched in various metabolic processes. Protein interaction network analysis using STRING identified inosine monophosphate dehydrogenase (IMPDH), Bifunctional GMP synthase/glutamine amidotransferase protein, Glucose-6-phosphate 1-dehydrogenase, and Citrate synthase as the top four hubs. Of the 22 virulence proteins commonly expressed in the oocysts of the two strains, 13 and 2 proteins were increased in PYS strain and PRU strain, respectively. Also, 10 and 3 of the 22 identified oocyst wall proteins showed higher expression in oocysts of PRU strain and PYS strain, respectively. These findings revealed new proteomic differences in the oocysts of T. gondii strains of different genotypic backgrounds.
Citation
Zhou, D.-H., Wang, Z.-X., Zhou, C.-X., He, S., Elsheikha, H. M., & Zhu, X.-Q. (2017). Comparative proteomic analysis of virulent and avirulent strains of Toxoplasma gondii reveals strain-specific patterns. Oncotarget, 8(46), https://doi.org/10.18632/oncotarget.19077
Journal Article Type | Article |
---|---|
Acceptance Date | Jun 18, 2017 |
Online Publication Date | Jul 7, 2017 |
Publication Date | Oct 6, 2017 |
Deposit Date | Jul 12, 2017 |
Publicly Available Date | Jul 12, 2017 |
Journal | Oncotarget |
Electronic ISSN | 1949-2553 |
Publisher | Impact Journals |
Peer Reviewed | Peer Reviewed |
Volume | 8 |
Issue | 46 |
DOI | https://doi.org/10.18632/oncotarget.19077 |
Public URL | https://nottingham-repository.worktribe.com/output/871721 |
Publisher URL | http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=19077&path[]=61141 |
Contract Date | Jul 12, 2017 |
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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
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