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Profiling of the perturbed metabolomic state of mouse spleen during acute and chronic toxoplasmosis

Chen, Xiao-Qing; Zhou, Chun-Xue; Elsheikha, Hany M.; He, Shuai; Hu, Gui-Xue; Zhu, Xing-Quan

Authors

Xiao-Qing Chen

Chun-Xue Zhou

Hany M. Elsheikha

Shuai He

Gui-Xue Hu

Xing-Quan Zhu



Abstract

Background

Toxoplasma gondii, a common opportunistic protozoan, is a leading cause of illness and mortality among immunosuppressed individuals and during congenital infections. Current therapeutic strategies for toxoplasmosis are not fully effective at curtailing disease progression in these cases. Given the parasite ability to influence host immunity and metabolism, understanding of the metabolic alterations in the host’s immune organs during T. gondii infection may enhance the understanding of the molecular mechanisms that define the pathophysiology of T. gondii infection.
Methods

We investigated the global metabolic changes in the spleen of BALB/c mice at early and late stage of infection with T. gondii using LC-MS/MS-based metabolomics. Multivariate data analysis methods, principal components analysis (PCA) and partial least squares discriminant analysis (PLS-DA), were used to identify metabolites that are influenced by T. gondii infection.
Results

Multivariate analyses clearly separated the metabolites of spleen of infected and control mice. A total of 132 differential metabolites were identified, 23 metabolites from acutely infected versus control mice and 109 metabolites from chronically infected versus control mice. Lipids, hormones, lactones, acids, peptides, antibiotics, alkaloids and natural toxins were the most influenced chemical groups. There were 12 shared differential metabolites between acutely infected versus control mice and chronically infected versus control mice, of which 4,4-Dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol was significantly upregulated and ubiquinone-8 was significantly downregulated. Major perturbed metabolic pathways included primary bile acid biosynthesis, steroid hormone biosynthesis, biotin metabolism, and steroid biosynthesis, with arachidonic acid metabolism being the most significantly impacted pathway. These metabolic changes suggest a multifactorial nature of the immunometabolic responses of mouse spleen to T. gondii infection.
Conclusions

This study demonstrated that T. gondii infection can cause significant metabolomic alterations in the spleen of infected mice. These findings provide new insights into the molecular mechanisms that underpin the pathogenesis of T. gondii infection.

Journal Article Type Article
Publication Date Jul 18, 2017
Journal Parasites & Vectors
Electronic ISSN 1756-3305
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 10
Issue 1
Article Number 339
APA6 Citation Chen, X., Zhou, C., Elsheikha, H. M., He, S., Hu, G., & Zhu, X. (2017). Profiling of the perturbed metabolomic state of mouse spleen during acute and chronic toxoplasmosis. Parasites and Vectors, 10(1), https://doi.org/10.1186/s13071-017-2282-6
DOI https://doi.org/10.1186/s13071-017-2282-6
Keywords Toxoplasma gondii; Spleen; Mass spectrometry; Metabolome; Non-targeted metabolomics; Pathway enrichment analysis
Publisher URL https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-017-2282-6
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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