All Outputs (50)

Disassembly of self-assembling peptide hydrogels as a versatile method for cell extraction and manipulation (2024)
Journal Article
Ligorio, C., Martinez-Espuga, M., Laurenza, D., Hartley, A., Rodgers, C. B., Kotowska, A. M., Scurr, D. J., Dalby, M. J., Ordóñez-Morán, P., & Mata, A. (2024). Disassembly of self-assembling peptide hydrogels as a versatile method for cell extraction and manipulation. Journal of Materials Chemistry B, https://doi.org/10.1039/D4TB01575D

Self-assembling peptide hydrogels (SAPHs) are increasingly being used as two-dimensional (2D) cell culture substrates and three-dimensional (3D) matrices due to their tunable properties and biomimicry of native tissues. Despite these advantages, SAPH... Read More about Disassembly of self-assembling peptide hydrogels as a versatile method for cell extraction and manipulation.

Cannabidiol and fluorinated derivative anti-cancer properties against glioblastoma multiforme cell lines, and synergy with imidazotetrazine agents (2024)
Journal Article
Brookes, A., Kindon, N., Scurr, D. J., Alexander, M. R., Gershkovich, P., & Bradshaw, T. (2024). Cannabidiol and fluorinated derivative anti-cancer properties against glioblastoma multiforme cell lines, and synergy with imidazotetrazine agents. BJC Reports, 2, Article 67. https://doi.org/10.1038/s44276-024-00088-0

Background
Glioblastoma multiforme (GBM) is an aggressive cancer with poor prognosis, partly due to resistance to the standard chemotherapy treatment, temozolomide (TMZ). Phytocannabinoid cannabidiol (CBD) has exhibited anti-cancer effects against G... Read More about Cannabidiol and fluorinated derivative anti-cancer properties against glioblastoma multiforme cell lines, and synergy with imidazotetrazine agents.

Cannabidiol and fluorinated derivative anti-cancer properties against glioblastoma multiforme cell lines, and synergy with imidazotetrazine agents (2024)
Journal Article
Brookes, A., Kindon, N., Scurr, D., Alexander, M., Gershkovich, P., & BRADSHAW, T. (in press). Cannabidiol and fluorinated derivative anti-cancer properties against glioblastoma multiforme cell lines, and synergy with imidazotetrazine agents. British Journal of Cancer, 2, Article 67

Background
Glioblastoma multiforme (GBM) is an aggressive cancer with poor prognosis, partly due to resistance to the standard chemotherapy treatment, temozolomide (TMZ). Phytocannabinoid cannabidiol (CBD) has exhibited anti-cancer effects against G... Read More about Cannabidiol and fluorinated derivative anti-cancer properties against glioblastoma multiforme cell lines, and synergy with imidazotetrazine agents.

084 Cosmetic peptide penetration and assembly in human skin: A label-free approach (2024)
Presentation / Conference Contribution
Ligorio, C., Kotowska, A., Scurr, D., Tavasoli, E., Karaman-Jurukovska, N., Mata, A., Moogan, L., German, G., Lu, F., & Mammone, T. (2024, May). 084 Cosmetic peptide penetration and assembly in human skin: A label-free approach. Presented at Society for Investigative Dermatology (SID) 2024 Meeting, Dallas, TX

Metabolomic and Proteomic Analysis of ApoE4-Carrying H4 Neuroglioma Cells in Alzheimer’s Disease Using OrbiSIMS and LC-MS/MS (2024)
Journal Article
Lu, L., Kotowska, A. M., Kern, S., Fang, M., Rudd, T. R., Alexander, M. R., Scurr, D. J., & Zhu, Z. (2024). Metabolomic and Proteomic Analysis of ApoE4-Carrying H4 Neuroglioma Cells in Alzheimer’s Disease Using OrbiSIMS and LC-MS/MS. Analytical Chemistry, https://doi.org/10.1021/acs.analchem.4c01201

Growing clinical evidence reveals that systematic molecular alterations in the brain occur 20 years before the onset of AD pathological features. Apolipoprotein E4 (ApoE4) is one of the most significant genetic risk factors for Alzheimer’s disease (A... Read More about Metabolomic and Proteomic Analysis of ApoE4-Carrying H4 Neuroglioma Cells in Alzheimer’s Disease Using OrbiSIMS and LC-MS/MS.

Spatially resolved molecular analysis of host response to medical device implantation using the 3D OrbiSIMS highlights a critical role for lipids (2024)
Journal Article
Suvannapruk, W., Fisher, L. E., Luckett, J. C., Edney, M. K., Kotowska, A. M., Kim, D. H., …Alexander, M. R. (2024). Spatially resolved molecular analysis of host response to medical device implantation using the 3D OrbiSIMS highlights a critical role for lipids. Advanced Science, 11(15), Article 2306000. https://doi.org/10.1002/advs.202306000

A key goal for implanted medical devices is that they do not elicit a detrimental immune response. Macrophages play critical roles in the modulation of the host immune response and are the cells responsible for persistent inflammatory reactions to im... Read More about Spatially resolved molecular analysis of host response to medical device implantation using the 3D OrbiSIMS highlights a critical role for lipids.

Targeted intradermal delivery of alpha-arbutin-loaded dissolving polymeric microneedles visualized by three-dimensional Orbitrap secondary ion mass spectrometry (3D OrbiSIMS) (2024)
Journal Article
Li, Z., Marlow, M., Scurr, D., & Zhu, Z. (2024). Targeted intradermal delivery of alpha-arbutin-loaded dissolving polymeric microneedles visualized by three-dimensional Orbitrap secondary ion mass spectrometry (3D OrbiSIMS). European Journal of Pharmaceutics and Biopharmaceutics, 196, Article 114181. https://doi.org/10.1016/j.ejpb.2024.114181

Hyperpigmentation, a prevalent dermatological condition characterized by melanin overproduction, poses treatment challenges due to the hydrophilicity of alpha-arbutin, a widely utilized tyrosinase inhibitor. This study investigates... Read More about Targeted intradermal delivery of alpha-arbutin-loaded dissolving polymeric microneedles visualized by three-dimensional Orbitrap secondary ion mass spectrometry (3D OrbiSIMS).

Investigating ApoE4‐mediated metabolic alteration of H4 neuroglioma cells of Alzheimer’s disease (2023)
Presentation / Conference Contribution
Lu, L., Kotowska, A., Kern, S., Fang, M., Rudd, T. R., Alexander, M., …Zhu, Z. (2023). Investigating ApoE4‐mediated metabolic alteration of H4 neuroglioma cells of Alzheimer’s disease. Alzheimer's & Dementia: The Journal of the Alzheimer's Association, 19(S24), Article e082458. https://doi.org/10.1002/alz.082458

Background
Metabolites and proteins in ApoE4‐carried H4 neuroglioma cells were analyzed to investigate the potential mechanism of the risk gene ApoE4 involved in Alzheimer’s disease (AD).

Method
Metabolomics of ApoE4‐carried and wild‐type H4 cel... Read More about Investigating ApoE4‐mediated metabolic alteration of H4 neuroglioma cells of Alzheimer’s disease.

Sustained Release of Dexamethasone from 3D-Printed Scaffolds Modulates Macrophage Activation and Enhances Osteogenic Differentiation (2023)
Journal Article
Majrashi, M., Yang, J., Ghaemmaghami, A., Kotowska, A., Hicks, J. M., & Scurr, D. (2023). Sustained Release of Dexamethasone from 3D-Printed Scaffolds Modulates Macrophage Activation and Enhances Osteogenic Differentiation. ACS Applied Materials and Interfaces, 15(49), 56623–56638. https://doi.org/10.1021/acsami.3c09774

Enhancing osteogenesis via modulating immune cells is emerging as a new approach to address current challenges in repairing bone defects and fractures. However, much remains unknown about the crosstalk between immune cells and osteolineage cells duri... Read More about Sustained Release of Dexamethasone from 3D-Printed Scaffolds Modulates Macrophage Activation and Enhances Osteogenic Differentiation.

Label-free Chemical Characterization of Polarized Immune Cells in vitro and Host Response to Implanted Bio-instructive Polymers in vivo Using 3D OrbiSIMS (2023)
Journal Article
Suvannapruk, W., Edney, M. K., Fisher, L. E., Luckett, J. C., Kim, D.-H., Scurr, D. J., Ghaemmaghami, A. M., & Alexander, M. R. (2023). Label-free Chemical Characterization of Polarized Immune Cells in vitro and Host Response to Implanted Bio-instructive Polymers in vivo Using 3D OrbiSIMS. Bio-protocol, 13(15), Article e4727. https://doi.org/10.21769/BioProtoc.4727

The Three-dimensional OrbiTrap Secondary Ion Mass Spectrometry (3D OrbiSIMS) is a secondary ion mass spectrometry instrument, a combination of a Time of Flight (ToF) instrument with an Orbitrap analyzer. The 3D OrbiSIMS technique is a powerful tool f... Read More about Label-free Chemical Characterization of Polarized Immune Cells in vitro and Host Response to Implanted Bio-instructive Polymers in vivo Using 3D OrbiSIMS.

Untargeted Metabolomic Characterization of Glioblastoma Intra-Tumor Heterogeneity Using OrbiSIMS (2023)
Journal Article
He, W., Edney, M. K., Paine, S. M. L., Griffiths, R. L., Scurr, D. J., Rahman, R., & Kim, D.-H. (2023). Untargeted Metabolomic Characterization of Glioblastoma Intra-Tumor Heterogeneity Using OrbiSIMS. Analytical Chemistry, 95(14), 5994-6001. https://doi.org/10.1021/acs.analchem.2c05807

Glioblastoma (GBM) is an incurable brain cancer with a median survival of less than two years from diagnosis. The standard treatment of GBM is multimodality therapy comprising surgical resection, radiation, and chemotherapy. However, prognosis remain... Read More about Untargeted Metabolomic Characterization of Glioblastoma Intra-Tumor Heterogeneity Using OrbiSIMS.

Design, Synthesis, and Biological Evaluation of Novel Hybrids Containing Dihydrochalcone as Tyrosinase Inhibitors to Treat Skin Hyperpigmentation (2023)
Journal Article
Xue, S., Li, Z., Ze, X., Wu, X., He, C., Shuai, W., …Xu, S. (2023). Design, Synthesis, and Biological Evaluation of Novel Hybrids Containing Dihydrochalcone as Tyrosinase Inhibitors to Treat Skin Hyperpigmentation. Journal of Medicinal Chemistry, 66(7), 5099–5117. https://doi.org/10.1021/acs.jmedchem.3c00012

Excessive melanin deposition may lead to a series of skin disorders. The production of melanin is carried out by melanocytes, in which the enzyme tyrosinase performs a key role. In this work, we identified a series of novel tyrosinase inhibitor hybri... Read More about Design, Synthesis, and Biological Evaluation of Novel Hybrids Containing Dihydrochalcone as Tyrosinase Inhibitors to Treat Skin Hyperpigmentation.

Identifying new biomarkers of aggressive Group 3 and SHH medulloblastoma using 3D hydrogel models, single cell RNA sequencing and 3D OrbiSIMS imaging (2023)
Journal Article
Linke, F., Johnson, J. E. C., Kern, S., Bennett, C. D., Lourdusamy, A., Lea, D., …Coyle, B. (2023). Identifying new biomarkers of aggressive Group 3 and SHH medulloblastoma using 3D hydrogel models, single cell RNA sequencing and 3D OrbiSIMS imaging. Acta Neuropathologica Communications, 11(1), Article 6. https://doi.org/10.1186/s40478-022-01496-4

The most common malignant brain tumour in children, medulloblastoma (MB), is subdivided into four clinically relevant molecular subgroups, although targeted therapy options informed by understanding of different cellular features are lacking. Here, b... Read More about Identifying new biomarkers of aggressive Group 3 and SHH medulloblastoma using 3D hydrogel models, single cell RNA sequencing and 3D OrbiSIMS imaging.

Development of nanoparticle loaded microneedles for drug delivery to a brain tumour resection site (2022)
Journal Article
Muresan, P., McCrorie, P., Smith, F., Vasey, C., Taresco, V., Scurr, D. J., …Marlow, M. (2023). Development of nanoparticle loaded microneedles for drug delivery to a brain tumour resection site. European Journal of Pharmaceutics and Biopharmaceutics, 182, 53-61. https://doi.org/10.1016/j.ejpb.2022.11.016

Systemic drug delivery to the central nervous system (CNS) has been historically impeded by the presence of the blood brain barrier rendering many therapies inefficacious to any cancer cells residing within the brain. Therefore, local drug delivery s... Read More about Development of nanoparticle loaded microneedles for drug delivery to a brain tumour resection site.

Metabolic alterations in dairy cattle with lameness revealed by untargeted metabolomics of dried milk spots using direct infusion-tandem mass spectrometry and the triangulation of multiple machine learning models (2022)
Journal Article
He, W., Cardoso, A. S., Hyde, R. M., Green, M. J., Scurr, D. J., Griffiths, R., …Kim, D.-H. (2022). Metabolic alterations in dairy cattle with lameness revealed by untargeted metabolomics of dried milk spots using direct infusion-tandem mass spectrometry and the triangulation of multiple machine learning models. Analyst, 147(23), 5537-5545. https://doi.org/10.1039/d2an01520j

Lameness is a major challenge in the dairy cattle industry in terms of animal welfare and economic implications. Better understanding of metabolic alteration associated with lameness could lead to early diagnosis and effective treatment{,} there-fore... Read More about Metabolic alterations in dairy cattle with lameness revealed by untargeted metabolomics of dried milk spots using direct infusion-tandem mass spectrometry and the triangulation of multiple machine learning models.

Single-Cell Metabolic Profiling of Macrophages Using 3D OrbiSIMS: Correlations with Phenotype (2022)
Journal Article
Suvannapruk, W., Edney, M. K., Kim, D., Scurr, D. J., Ghaemmaghami, A. M., & Alexander, M. R. (2022). Single-Cell Metabolic Profiling of Macrophages Using 3D OrbiSIMS: Correlations with Phenotype. Analytical Chemistry, 94(26), 9389–9398. https://doi.org/10.1021/acs.analchem.2c01375

Macrophages are important immune cells that respond to environmental cues acquiring a range of activation statuses represented by pro-inflammatory (M1) and anti-inflammatory (M2) phenotypes at each end of their spectrum. Characterizing the metabolic... Read More about Single-Cell Metabolic Profiling of Macrophages Using 3D OrbiSIMS: Correlations with Phenotype.

Molecular Formula Prediction for Chemical Filtering of 3D OrbiSIMS Datasets (2022)
Journal Article
Edney, M. K., Kotowska, A. M., Spanu, M., Trindade, G. F., Wilmot, E., Reid, J., …Scurr, D. J. (2022). Molecular Formula Prediction for Chemical Filtering of 3D OrbiSIMS Datasets. Analytical Chemistry, 94(11), 4703–4711. https://doi.org/10.1021/acs.analchem.1c04898

Modern mass spectrometry techniques produce a wealth of spectral data, and although this is an advantage in terms of the richness of the information available, the volume and complexity of data can prevent a thorough interpretation to reach useful co... Read More about Molecular Formula Prediction for Chemical Filtering of 3D OrbiSIMS Datasets.

Efficacy of antimicrobial and anti-viral coated air filters to prevent the spread of airborne pathogens (2022)
Journal Article
Watson, R., Oldfield, M., Bryant, J. A., Riordan, L., Hill, H. J., Watts, J. A., …de Cogan, F. (2022). Efficacy of antimicrobial and anti-viral coated air filters to prevent the spread of airborne pathogens. Scientific Reports, 12(1), Article 2803. https://doi.org/10.1038/s41598-022-06579-9

The COVID-19 pandemic has demonstrated the real need for mechanisms to control the spread of airborne respiratory pathogens. Thus, preventing the spread of disease from pathogens has come to the forefront of the public consciousness. This has brought... Read More about Efficacy of antimicrobial and anti-viral coated air filters to prevent the spread of airborne pathogens.

Detection of Label-Free Drugs within Brain Tissue Using Orbitrap Secondary Ion Mass Spectrometry as a Complement to Neuro-Oncological Drug Delivery (2022)
Journal Article
McCrorie, P., Rowlinson, J., Scurr, D. J., Marlow, M., & Rahman, R. (2022). Detection of Label-Free Drugs within Brain Tissue Using Orbitrap Secondary Ion Mass Spectrometry as a Complement to Neuro-Oncological Drug Delivery. Pharmaceutics, 14(3), Article 571. https://doi.org/10.3390/pharmaceutics14030571

Historically, pre-clinical neuro-oncological drug delivery studies have exhaustively relied upon overall animal survival as an exclusive measure of efficacy. However, with no adopted methodology to both image and quantitate brain parenchyma penetrati... Read More about Detection of Label-Free Drugs within Brain Tissue Using Orbitrap Secondary Ion Mass Spectrometry as a Complement to Neuro-Oncological Drug Delivery.

Quantifiable correlation of ToF-SIMS and XPS data from polymer surfaces with controlled amino acid and peptide content (2022)
Journal Article
Taylor, M., Simoes, F., Smith, J., Genapathy, S., Canning, A., Lledos, M., …Zelzer, M. (2022). Quantifiable correlation of ToF-SIMS and XPS data from polymer surfaces with controlled amino acid and peptide content. Surface and Interface Analysis, 54(4), 417-432. https://doi.org/10.1002/sia.7052

Peptide-coated surfaces are widely employed in biomaterial design, but quantifiable correlation between surface composition and biological response is challenging due to, for example, instrumental limitations, a lack of suitable model surfaces or lim... Read More about Quantifiable correlation of ToF-SIMS and XPS data from polymer surfaces with controlled amino acid and peptide content.