Paula Muresan
Development of nanoparticle loaded microneedles for drug delivery to a brain tumour resection site
Muresan, Paula; McCrorie, Phoebe; Smith, Fiona; Vasey, Catherine; Taresco, Vincenzo; Scurr, David J; Kern, Stefanie; Smith, Stuart; Gershkovich, Pavel; Rahman, Ruman; Marlow, Maria
Authors
Dr Phoebe McCrorie PHOEBE.MCCRORIE1@NOTTINGHAM.AC.UK
RESEARCH FELLOW
Fiona Smith
Catherine Vasey
Dr VINCENZO TARESCO VINCENZO.TARESCO@NOTTINGHAM.AC.UK
NOTTINGHAM RESEARCH FELLOW
Dr DAVID SCURR DAVID.SCURR@NOTTINGHAM.AC.UK
PRINCIPAL RESEARCH FELLOW
Stefanie Kern
Dr STUART SMITH stuart.smith@nottingham.ac.uk
CLINICAL ASSOCIATE PROFESSOR
Dr PAVEL GERSHKOVICH PAVEL.GERSHKOVICH@NOTTINGHAM.AC.UK
ASSOCIATE PROFESSOR
Professor Ruman Rahman RUMAN.RAHMAN@NOTTINGHAM.AC.UK
PROFESSOR OF MOLECULAR NEURO-ONCOLOGY
Dr MARIA MARLOW Maria.Marlow@nottingham.ac.uk
ASSOCIATE PROFESSOR
Abstract
Systemic drug delivery to the central nervous system (CNS) has been historically impeded by the presence of the blood brain barrier rendering many therapies inefficacious to any cancer cells residing within the brain. Therefore, local drug delivery systems are being developed to overcome this shortfall. Here we have manufactured polymeric microneedle (MN) patches, which can be anchored within a resection cavity site following surgical removal of a tumour such as isocitrate dehydrogenase wild type glioblastoma (GBM). These biodegradable MN patches have been loaded with polymer coated nanoparticles (NPs) containing cannabidiol (CBD) or olaparib (OLA) and applied to an in vitro brain simulant and ex vivo rat brain tissue to assess drug release and distance of penetration. MN patches loaded with methylene blue dye were placed into a cavity of 0.6% agarose to simulate brain tissue. The results showed that clear channels were generated by the MNs and the dye spread laterally throughout the agarose. When loaded with CBD-NPs, the agarose showed a CBD concentration of 12.5 µg/g at 0.5 cm from the MN insertion site. Furthermore, high performance liquid chromatography of ex vivo brain tissue following CBD-NP/MN patch insertion showed successful delivery of 59.6 µg/g into the brain tissue. Similarly, OLA-NP loaded MN patches showed delivery of 5.2 µg/g OLA into agarose gel at 0.5 cm distance from the insertion site. Orbitrap secondary ion mass spectrometry (OrbiSIMS) analysis confirmed the presence of OLA and the MN patch at up to 6 mm away from the insertion site following its application to a rat brain hemisphere. This data has provided insight into the capabilities and versatility of MN patches for use in local brain drug delivery, giving promise for future research.
Citation
Muresan, P., McCrorie, P., Smith, F., Vasey, C., Taresco, V., Scurr, D. J., Kern, S., Smith, S., Gershkovich, P., Rahman, R., & Marlow, M. (2023). Development of nanoparticle loaded microneedles for drug delivery to a brain tumour resection site. European Journal of Pharmaceutics and Biopharmaceutics, 182, 53-61. https://doi.org/10.1016/j.ejpb.2022.11.016
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 16, 2022 |
Online Publication Date | Nov 24, 2022 |
Publication Date | 2023-01 |
Deposit Date | Dec 1, 2022 |
Publicly Available Date | Nov 25, 2023 |
Journal | European Journal of Pharmaceutics and Biopharmaceutics |
Print ISSN | 0939-6411 |
Electronic ISSN | 1873-3441 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 182 |
Pages | 53-61 |
DOI | https://doi.org/10.1016/j.ejpb.2022.11.016 |
Keywords | Microneedles, nanoparticles, isocitrate dehydrogenase wild type glioblastoma |
Public URL | https://nottingham-repository.worktribe.com/output/14315663 |
Publisher URL | https://www.sciencedirect.com/science/article/abs/pii/S0939641122002752?via%3Dihub |
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