All Outputs (8)

Multifunctional Bioinstructive 3D Architectures to Modulate Cellular Behavior (2019)
Journal Article
Vaithilingam, J., Sanjuan‐Alberte, P., Campora, S., Rance, G. A., Jiang, L., Thorpe, J., …Rawson, F. J. (2019). Multifunctional Bioinstructive 3D Architectures to Modulate Cellular Behavior. Advanced Functional Materials, 29(38), Article 1902016. https://doi.org/10.1002/adfm.201902016

Biological structures control cell behavior via physical, chemical, electrical, and mechanical cues. Approaches that allow us to build devices that mimic these cues in a combinatorial way are lacking due to there being no suitable instructive materia... Read More about Multifunctional Bioinstructive 3D Architectures to Modulate Cellular Behavior.

Automated electrophysiological and pharmacological evaluation of human pluripotent stem cell-derived cardiomyocytes (2016)
Journal Article
Rajamohan, D., Kalra, S., Hoang, M. D., George, V., Staniforth, A., Russell, H., …Denning, C. (2016). Automated electrophysiological and pharmacological evaluation of human pluripotent stem cell-derived cardiomyocytes. Stem Cells and Development, 25(6), https://doi.org/10.1089/scd.2015.0253

Automated planar patch clamp systems are widely used in drug evaluation studies because of their ability to provide accurate, reliable, and reproducible data in a high-throughput manner. Typically, CHO and HEK tumorigenic cell lines overexpressing si... Read More about Automated electrophysiological and pharmacological evaluation of human pluripotent stem cell-derived cardiomyocytes.

Cardiomyocytes from human pluripotent stem cells: from laboratory curiosity to industrial biomedical platform (2015)
Journal Article
Denning, C., Borgdorff, V., Crutchley, J., Firth, K. S., George, V., Kalra, S., …Young, L. E. (2016). Cardiomyocytes from human pluripotent stem cells: from laboratory curiosity to industrial biomedical platform. BBA - Biochimica et Biophysica Acta, 1863(7), https://doi.org/10.1016/j.bbamcr.2015.10.014

Cardiomyocytes from human pluripotent stem cells (hPSCs-CMs) could revolutionise biomedicine. Global burden of heart failure will soon reach USD $90bn, while unexpected cardiotoxicity underlies 28% of drug withdrawals. Advances in hPSC isolation, Cas... Read More about Cardiomyocytes from human pluripotent stem cells: from laboratory curiosity to industrial biomedical platform.

Discovery of a Novel Polymer for Human Pluripotent Stem Cell Expansion and Multilineage Differentiation (2015)
Journal Article
Smith, J. G. W., Celiz, A. D., Smith, J. G., Patel, A. K., Hook, A. L., Rajamohan, D., …Alexander, M. R. (2015). Discovery of a Novel Polymer for Human Pluripotent Stem Cell Expansion and Multilineage Differentiation. Advanced Materials, 27(27), 4006-4012. https://doi.org/10.1002/adma.201501351

© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. A scalable and cost-effective synthetic polymer substrate that supports robust expansion and subsequent multilineage differentiation of human pluripotent stem cells (hPSCs) with defined commercial... Read More about Discovery of a Novel Polymer for Human Pluripotent Stem Cell Expansion and Multilineage Differentiation.

Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation (2014)
Journal Article
Dixon, J. E., Shah, D. A., Rogers, C., Hall, S., Weston, N., Parmenter, C. D., …Shakesheff, K. M. (2014). Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation. Proceedings of the National Academy of Sciences, 111(15), 5580-5585. https://doi.org/10.1073/pnas.1319685111

The ability of materials to define the architecture and microenvironment experienced by cells provides new opportunities to direct the fate of human pluripotent stem cells (HPSCs) [Robinton DA, Daley GQ (2012) Nature 481(7381):295-305]. However, the... Read More about Combined hydrogels that switch human pluripotent stem cells from self-renewal to differentiation.

Exon skipping and gene transfer restore dystrophin expression in human induced pluripotent stem cells-cardiomyocytes harboring DMD mutations (2013)
Journal Article
Dick, E., Kalra, S., Anderson, D., George, V., Ritso, M., Laval, S. H., …Denning, C. (2013). Exon skipping and gene transfer restore dystrophin expression in human induced pluripotent stem cells-cardiomyocytes harboring DMD mutations. Stem Cells and Development, 22(20), https://doi.org/10.1089/scd.2013.0135

With an incidence of 1:3,500 to 5,000 in male children, Duchenne muscular dystrophy (DMD) is an X-linked disorder in which progressive muscle degeneration occurs and affected boys usually die in their twenties or thirties. Cardiac involvement occurs... Read More about Exon skipping and gene transfer restore dystrophin expression in human induced pluripotent stem cells-cardiomyocytes harboring DMD mutations.

Allele-specific RNA interference rescues the long-QT syndrome phenotype in human-induced pluripotency stem cell cardiomyocytes (2013)
Journal Article
Matsa, E., Dixon, J. E., Medway, C., Georgiou, O., Patel, M. J., Morgan, K., …Denning, C. (2014). Allele-specific RNA interference rescues the long-QT syndrome phenotype in human-induced pluripotency stem cell cardiomyocytes. European Heart Journal, 35(16), 1078-1087. https://doi.org/10.1093/eurheartj/eht067

Aims Long-QT syndromes (LQTS) are mostly autosomal-dominant congenital disorders associated with a 1:1000 mutation frequency, cardiac arrest, and sudden death. We sought to use cardiomyocytes derived from human-induced pluripotency stem cells (hiPSCs... Read More about Allele-specific RNA interference rescues the long-QT syndrome phenotype in human-induced pluripotency stem cell cardiomyocytes.

Current status of drug screening and disease modelling in human pluripotent stem cells (2013)
Journal Article
Rajamohan, D., Matsa, E., Kalra, S., Crutchley, J., Patel, A., George, V., & Denning, C. (2013). Current status of drug screening and disease modelling in human pluripotent stem cells. BioEssays, 35(3), https://doi.org/10.1002/bies.201200053

The emphasis in human pluripotent stem cell (hPSC) technologies has shifted from cell therapy to in vitro disease modelling and drug screening. This review examines why this shift has occurred, and how current technological limitations might be overc... Read More about Current status of drug screening and disease modelling in human pluripotent stem cells.